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Effects of myenteric denervation on extracellular matrix fibers and mast cell distribution in normal stomach and gastric lesions.

Estofolete CF, Botelho-Machado C, Taboga SR, Zucoloto S, Polli-Lopes AC, Gil CD - Cancer Cell Int. (2010)

Bottom Line: An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers.The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anatomy, School of Medicine - FAMERP, Avenida Brigadeiro Faria Lima 5416, CEP 15090-000, São José do Rio Preto, SP, Brazil. cristiane@famerp.br.

ABSTRACT

Background: In this study the effect of myenteric denervation induced by benzalconium chloride (BAC) on distribution of fibrillar components of extracellular matrix (ECM) and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Rats were divided in four experimental groups: non-denervated (I) and denervated stomach (II) without MNNG treatment; non-denervated (III) and denervated stomachs (IV) treated with MNNG. For histopathological, histochemical and stereological analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert's Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue/Safranin (AB-SAF).

Results: BAC denervation causes an increase in the frequency of reticular and elastic fibers in the denervated (group II) compared to the non-denervated stomachs (group I). The treatment of the animals with MNNG induced the development of adenocarcinomas in non-denervated and denervated stomachs (groups III and IV, respectively) with a notable increase in the relative volume of the stroma, the frequency of reticular fibers and the inflammatory infiltrate that was more intense in group IV. An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers. The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.

Conclusions: The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC. This suggests that the study of extracellular and intracellular components of tumor microenvironment contributes to understanding of tumor biology by action of myenteric denervation.

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Quantitative analysis of mast cells in the stomach. Data represent the mean ± S.E.M of sixty fields analyzed per animal as described in Material and Methods. Non-denervated (I) and denervated stomach (II) without lesions. Non-denervated (III) and denervated stomach (IV) with lesions. Non-malignant tumor (NT). Malignant tumor (MT). A) Total number of mast cells in the gastric fragment. *P < 0.05 vs. group I. # P < 0.01 vs. another experimental groups. B and C) Intact and degranulated mast cells in the mucosal, submucosal and muscular layers. * P < 0.05 vs. group I; #P < 0.05 vs. another experimental groups; δ P < 0.05 vs. group III - NT (intact mast cells); &P < 0.05 vs. group III - NT (degranulated mast cells). C) Intact and degranulated mast cells in the muscular layer. *P < 0.05 vs. group I; # P < 0.05 vs. group II.
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Figure 4: Quantitative analysis of mast cells in the stomach. Data represent the mean ± S.E.M of sixty fields analyzed per animal as described in Material and Methods. Non-denervated (I) and denervated stomach (II) without lesions. Non-denervated (III) and denervated stomach (IV) with lesions. Non-malignant tumor (NT). Malignant tumor (MT). A) Total number of mast cells in the gastric fragment. *P < 0.05 vs. group I. # P < 0.01 vs. another experimental groups. B and C) Intact and degranulated mast cells in the mucosal, submucosal and muscular layers. * P < 0.05 vs. group I; #P < 0.05 vs. another experimental groups; δ P < 0.05 vs. group III - NT (intact mast cells); &P < 0.05 vs. group III - NT (degranulated mast cells). C) Intact and degranulated mast cells in the muscular layer. *P < 0.05 vs. group I; # P < 0.05 vs. group II.

Mentions: Mast cells were analyzed and quantified in the mucosal, submucosal and muscular layers in all experimental groups. The analysis of the total number of cells per gastric fragment showed no significant difference between the non-denervated (I) and denervated (II) groups (Fig. 4A). However, in non-denervated or denervated groups treated with MNNG (III and IV, respectively), the development of benign or malignant lesions was associated with a significant increase of the number of mast cells in the gastric antrum compared with the non-denervated group (I). In the mucosal and submucosal layers, the significant increase of mast cells in the non-denervated or denervated groups treated with MNNG, (III and IV) was associated with an increase of intact and degranulated cells (Fig. 4B). In the muscle layer, this increase was mainly associated with the large number of intact mast cells (Fig. 4C).


Effects of myenteric denervation on extracellular matrix fibers and mast cell distribution in normal stomach and gastric lesions.

Estofolete CF, Botelho-Machado C, Taboga SR, Zucoloto S, Polli-Lopes AC, Gil CD - Cancer Cell Int. (2010)

Quantitative analysis of mast cells in the stomach. Data represent the mean ± S.E.M of sixty fields analyzed per animal as described in Material and Methods. Non-denervated (I) and denervated stomach (II) without lesions. Non-denervated (III) and denervated stomach (IV) with lesions. Non-malignant tumor (NT). Malignant tumor (MT). A) Total number of mast cells in the gastric fragment. *P < 0.05 vs. group I. # P < 0.01 vs. another experimental groups. B and C) Intact and degranulated mast cells in the mucosal, submucosal and muscular layers. * P < 0.05 vs. group I; #P < 0.05 vs. another experimental groups; δ P < 0.05 vs. group III - NT (intact mast cells); &P < 0.05 vs. group III - NT (degranulated mast cells). C) Intact and degranulated mast cells in the muscular layer. *P < 0.05 vs. group I; # P < 0.05 vs. group II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908609&req=5

Figure 4: Quantitative analysis of mast cells in the stomach. Data represent the mean ± S.E.M of sixty fields analyzed per animal as described in Material and Methods. Non-denervated (I) and denervated stomach (II) without lesions. Non-denervated (III) and denervated stomach (IV) with lesions. Non-malignant tumor (NT). Malignant tumor (MT). A) Total number of mast cells in the gastric fragment. *P < 0.05 vs. group I. # P < 0.01 vs. another experimental groups. B and C) Intact and degranulated mast cells in the mucosal, submucosal and muscular layers. * P < 0.05 vs. group I; #P < 0.05 vs. another experimental groups; δ P < 0.05 vs. group III - NT (intact mast cells); &P < 0.05 vs. group III - NT (degranulated mast cells). C) Intact and degranulated mast cells in the muscular layer. *P < 0.05 vs. group I; # P < 0.05 vs. group II.
Mentions: Mast cells were analyzed and quantified in the mucosal, submucosal and muscular layers in all experimental groups. The analysis of the total number of cells per gastric fragment showed no significant difference between the non-denervated (I) and denervated (II) groups (Fig. 4A). However, in non-denervated or denervated groups treated with MNNG (III and IV, respectively), the development of benign or malignant lesions was associated with a significant increase of the number of mast cells in the gastric antrum compared with the non-denervated group (I). In the mucosal and submucosal layers, the significant increase of mast cells in the non-denervated or denervated groups treated with MNNG, (III and IV) was associated with an increase of intact and degranulated cells (Fig. 4B). In the muscle layer, this increase was mainly associated with the large number of intact mast cells (Fig. 4C).

Bottom Line: An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers.The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anatomy, School of Medicine - FAMERP, Avenida Brigadeiro Faria Lima 5416, CEP 15090-000, São José do Rio Preto, SP, Brazil. cristiane@famerp.br.

ABSTRACT

Background: In this study the effect of myenteric denervation induced by benzalconium chloride (BAC) on distribution of fibrillar components of extracellular matrix (ECM) and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Rats were divided in four experimental groups: non-denervated (I) and denervated stomach (II) without MNNG treatment; non-denervated (III) and denervated stomachs (IV) treated with MNNG. For histopathological, histochemical and stereological analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert's Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue/Safranin (AB-SAF).

Results: BAC denervation causes an increase in the frequency of reticular and elastic fibers in the denervated (group II) compared to the non-denervated stomachs (group I). The treatment of the animals with MNNG induced the development of adenocarcinomas in non-denervated and denervated stomachs (groups III and IV, respectively) with a notable increase in the relative volume of the stroma, the frequency of reticular fibers and the inflammatory infiltrate that was more intense in group IV. An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers. The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.

Conclusions: The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC. This suggests that the study of extracellular and intracellular components of tumor microenvironment contributes to understanding of tumor biology by action of myenteric denervation.

No MeSH data available.


Related in: MedlinePlus