Limits...
Effects of myenteric denervation on extracellular matrix fibers and mast cell distribution in normal stomach and gastric lesions.

Estofolete CF, Botelho-Machado C, Taboga SR, Zucoloto S, Polli-Lopes AC, Gil CD - Cancer Cell Int. (2010)

Bottom Line: An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers.The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anatomy, School of Medicine - FAMERP, Avenida Brigadeiro Faria Lima 5416, CEP 15090-000, São José do Rio Preto, SP, Brazil. cristiane@famerp.br.

ABSTRACT

Background: In this study the effect of myenteric denervation induced by benzalconium chloride (BAC) on distribution of fibrillar components of extracellular matrix (ECM) and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Rats were divided in four experimental groups: non-denervated (I) and denervated stomach (II) without MNNG treatment; non-denervated (III) and denervated stomachs (IV) treated with MNNG. For histopathological, histochemical and stereological analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert's Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue/Safranin (AB-SAF).

Results: BAC denervation causes an increase in the frequency of reticular and elastic fibers in the denervated (group II) compared to the non-denervated stomachs (group I). The treatment of the animals with MNNG induced the development of adenocarcinomas in non-denervated and denervated stomachs (groups III and IV, respectively) with a notable increase in the relative volume of the stroma, the frequency of reticular fibers and the inflammatory infiltrate that was more intense in group IV. An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers. The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.

Conclusions: The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC. This suggests that the study of extracellular and intracellular components of tumor microenvironment contributes to understanding of tumor biology by action of myenteric denervation.

No MeSH data available.


Related in: MedlinePlus

Histological analysis of rat stomachs. A, B, E and F) Pyloric gastric glands (g) surrounded by stroma with collagen fibers (s) observed in the mucosa layer of the groups I and II. Epithelium (ep). Gastric pits (f). Muscularis mucosa (m). C, D, G and H) Pyloric gastric glands (g) constituted by tumoral cells occupied the largest area of the gastric mucosa of the groups III and IV. An accumulation of PMN (arrows) was observed within the glands. Hematoxylin-Eosin (A-D); Picrosirius-Hematoxylin (E-H). Bars: 20 μm. I-L) Tissue compartments (epithelium, stroma, others) volumes of the gastric pyloric mucosa. Values are expressed as mean ± SEM of percentage of relative volume. Groups without lesions (I and II) presented a high relative volume of epithelium (p < 0.05) compared to groups with lesions (III and IV). The presence of adenocarcinoma in the groups III and IV resulted in a significant increase (p < 0.05) of stroma compared to groups I and II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2908609&req=5

Figure 1: Histological analysis of rat stomachs. A, B, E and F) Pyloric gastric glands (g) surrounded by stroma with collagen fibers (s) observed in the mucosa layer of the groups I and II. Epithelium (ep). Gastric pits (f). Muscularis mucosa (m). C, D, G and H) Pyloric gastric glands (g) constituted by tumoral cells occupied the largest area of the gastric mucosa of the groups III and IV. An accumulation of PMN (arrows) was observed within the glands. Hematoxylin-Eosin (A-D); Picrosirius-Hematoxylin (E-H). Bars: 20 μm. I-L) Tissue compartments (epithelium, stroma, others) volumes of the gastric pyloric mucosa. Values are expressed as mean ± SEM of percentage of relative volume. Groups without lesions (I and II) presented a high relative volume of epithelium (p < 0.05) compared to groups with lesions (III and IV). The presence of adenocarcinoma in the groups III and IV resulted in a significant increase (p < 0.05) of stroma compared to groups I and II.

Mentions: Initially, pyloric fragments of non-denervated and denervated stomachs by benzalkonium chloride (BAC) (groups I and II, respectively) were studied. Analysis of fragments of group II showed that myenteric denervation didn't change the quantity and distribution of the meshwork collagen fibers that surround the pyloric glands compared to group I (Fig. 1A and 1B). This result was confirmed by Picrosirius-Hematoxylin stain (Fig. 1E and 1F) and quantitative analysis of the relative volume of the epithelium and stroma of the gastric mucosa from both groups (Fig. 1I and 1J).


Effects of myenteric denervation on extracellular matrix fibers and mast cell distribution in normal stomach and gastric lesions.

Estofolete CF, Botelho-Machado C, Taboga SR, Zucoloto S, Polli-Lopes AC, Gil CD - Cancer Cell Int. (2010)

Histological analysis of rat stomachs. A, B, E and F) Pyloric gastric glands (g) surrounded by stroma with collagen fibers (s) observed in the mucosa layer of the groups I and II. Epithelium (ep). Gastric pits (f). Muscularis mucosa (m). C, D, G and H) Pyloric gastric glands (g) constituted by tumoral cells occupied the largest area of the gastric mucosa of the groups III and IV. An accumulation of PMN (arrows) was observed within the glands. Hematoxylin-Eosin (A-D); Picrosirius-Hematoxylin (E-H). Bars: 20 μm. I-L) Tissue compartments (epithelium, stroma, others) volumes of the gastric pyloric mucosa. Values are expressed as mean ± SEM of percentage of relative volume. Groups without lesions (I and II) presented a high relative volume of epithelium (p < 0.05) compared to groups with lesions (III and IV). The presence of adenocarcinoma in the groups III and IV resulted in a significant increase (p < 0.05) of stroma compared to groups I and II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908609&req=5

Figure 1: Histological analysis of rat stomachs. A, B, E and F) Pyloric gastric glands (g) surrounded by stroma with collagen fibers (s) observed in the mucosa layer of the groups I and II. Epithelium (ep). Gastric pits (f). Muscularis mucosa (m). C, D, G and H) Pyloric gastric glands (g) constituted by tumoral cells occupied the largest area of the gastric mucosa of the groups III and IV. An accumulation of PMN (arrows) was observed within the glands. Hematoxylin-Eosin (A-D); Picrosirius-Hematoxylin (E-H). Bars: 20 μm. I-L) Tissue compartments (epithelium, stroma, others) volumes of the gastric pyloric mucosa. Values are expressed as mean ± SEM of percentage of relative volume. Groups without lesions (I and II) presented a high relative volume of epithelium (p < 0.05) compared to groups with lesions (III and IV). The presence of adenocarcinoma in the groups III and IV resulted in a significant increase (p < 0.05) of stroma compared to groups I and II.
Mentions: Initially, pyloric fragments of non-denervated and denervated stomachs by benzalkonium chloride (BAC) (groups I and II, respectively) were studied. Analysis of fragments of group II showed that myenteric denervation didn't change the quantity and distribution of the meshwork collagen fibers that surround the pyloric glands compared to group I (Fig. 1A and 1B). This result was confirmed by Picrosirius-Hematoxylin stain (Fig. 1E and 1F) and quantitative analysis of the relative volume of the epithelium and stroma of the gastric mucosa from both groups (Fig. 1I and 1J).

Bottom Line: An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers.The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anatomy, School of Medicine - FAMERP, Avenida Brigadeiro Faria Lima 5416, CEP 15090-000, São José do Rio Preto, SP, Brazil. cristiane@famerp.br.

ABSTRACT

Background: In this study the effect of myenteric denervation induced by benzalconium chloride (BAC) on distribution of fibrillar components of extracellular matrix (ECM) and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Rats were divided in four experimental groups: non-denervated (I) and denervated stomach (II) without MNNG treatment; non-denervated (III) and denervated stomachs (IV) treated with MNNG. For histopathological, histochemical and stereological analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert's Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue/Safranin (AB-SAF).

Results: BAC denervation causes an increase in the frequency of reticular and elastic fibers in the denervated (group II) compared to the non-denervated stomachs (group I). The treatment of the animals with MNNG induced the development of adenocarcinomas in non-denervated and denervated stomachs (groups III and IV, respectively) with a notable increase in the relative volume of the stroma, the frequency of reticular fibers and the inflammatory infiltrate that was more intense in group IV. An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated (group IV) compared to the non-denervated stomachs (group III) that showed degradation of these fibers. The development of lesions (groups III and IV) was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.

Conclusions: The results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC. This suggests that the study of extracellular and intracellular components of tumor microenvironment contributes to understanding of tumor biology by action of myenteric denervation.

No MeSH data available.


Related in: MedlinePlus