Limits...
Arginase levels and their association with Th17-related cytokines, soluble adhesion molecules (sICAM-1 and sVCAM-1) and hemolysis markers among steady-state sickle cell anemia patients.

Vilas-Boas W, Cerqueira BA, Zanette AM, Reis MG, Barral-Netto M, Goncalves MS - Ann. Hematol. (2010)

Bottom Line: Moreover, arginase was significantly and positively associated with transforming growth factor-beta (TGF-beta; p = 0.008) among SCA patients. sICAM-1 was significantly and positively associated to reticulocytes (p = 0.014) and AST (p = 0.04). sVCAM-1 was likewise associated with lactate dehydrogenase (p = 0.03).These data suggest a new insight into arginase metabolism, as we show here a shift in arginine catabolism, where TGF-beta may induces the arginase pathway instead of the nitric oxide pathway and a possible involvement of the vascular activation and the serum arginase in chronic hemolysis among SCA patients.Additional studies should be carried out in order to investigate the mechanisms by which TGF-beta participates in the metabolism of arginase in SCA patients.

View Article: PubMed Central - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz/FIOCRUZ, Rua Waldemar Falcão 121. Brotas, Salvador, Bahia, Brazil.

ABSTRACT
Sickle cell anemia (SCA) is characterized by a marked endothelial dysfunction, owing to many factors. Arginine metabolism can be related to the inflammatory chronic state presented by patients, playing a key role in their clinical outcome and vascular endothelium. We investigated the serum arginase levels in 50 SCA patients (22 men and 28 women, mean age of 17 +/- 10.5 years) and 28 healthy controls. Serum arginase levels were associated with biochemical hemolysis markers and cytokines involved in Th17 response, as well as levels of soluble intercellular adhesion molecule-1 (sICAM-1) and vascular cell adhesion molecule-1 (sVCAM-1). Arginase concentrations were higher in SCA patients, compared with controls (p = 0.005), and were significantly and positively associated with total bilirubin (p = 0.004), indirect bilirubin (p = 0.04), and aspartate aminotransferase (AST; p = 0.039) in the SCA patient group. Moreover, arginase was significantly and positively associated with transforming growth factor-beta (TGF-beta; p = 0.008) among SCA patients. sICAM-1 was significantly and positively associated to reticulocytes (p = 0.014) and AST (p = 0.04). sVCAM-1 was likewise associated with lactate dehydrogenase (p = 0.03). These data suggest a new insight into arginase metabolism, as we show here a shift in arginine catabolism, where TGF-beta may induces the arginase pathway instead of the nitric oxide pathway and a possible involvement of the vascular activation and the serum arginase in chronic hemolysis among SCA patients. Additional studies should be carried out in order to investigate the mechanisms by which TGF-beta participates in the metabolism of arginase in SCA patients.

Show MeSH

Related in: MedlinePlus

Linear regression of arginase serum (ng/mL) and hemolysis markers in sickle cell anemia patients
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2908460&req=5

Fig2: Linear regression of arginase serum (ng/mL) and hemolysis markers in sickle cell anemia patients

Mentions: In this work, we investigated cytokines involved in Th17 response and arginase expression regulation (e.g., IL-23, IL-17, and TGF-beta), associating these with free arginase in SCA individuals. There was a positive and significant association between arginase and TGF-beta (p = 0.008, r = 0.588), as shown in Table 2. The linear regression of arginase levels was also positively and significantly associated with AST (p < 0.0001, r = 0.522), total bilirubin (p = 0.034, r = 0.301), and indirect bilirubin (p = 0.039, r = 0.293; Fig. 2).Table 2


Arginase levels and their association with Th17-related cytokines, soluble adhesion molecules (sICAM-1 and sVCAM-1) and hemolysis markers among steady-state sickle cell anemia patients.

Vilas-Boas W, Cerqueira BA, Zanette AM, Reis MG, Barral-Netto M, Goncalves MS - Ann. Hematol. (2010)

Linear regression of arginase serum (ng/mL) and hemolysis markers in sickle cell anemia patients
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2908460&req=5

Fig2: Linear regression of arginase serum (ng/mL) and hemolysis markers in sickle cell anemia patients
Mentions: In this work, we investigated cytokines involved in Th17 response and arginase expression regulation (e.g., IL-23, IL-17, and TGF-beta), associating these with free arginase in SCA individuals. There was a positive and significant association between arginase and TGF-beta (p = 0.008, r = 0.588), as shown in Table 2. The linear regression of arginase levels was also positively and significantly associated with AST (p < 0.0001, r = 0.522), total bilirubin (p = 0.034, r = 0.301), and indirect bilirubin (p = 0.039, r = 0.293; Fig. 2).Table 2

Bottom Line: Moreover, arginase was significantly and positively associated with transforming growth factor-beta (TGF-beta; p = 0.008) among SCA patients. sICAM-1 was significantly and positively associated to reticulocytes (p = 0.014) and AST (p = 0.04). sVCAM-1 was likewise associated with lactate dehydrogenase (p = 0.03).These data suggest a new insight into arginase metabolism, as we show here a shift in arginine catabolism, where TGF-beta may induces the arginase pathway instead of the nitric oxide pathway and a possible involvement of the vascular activation and the serum arginase in chronic hemolysis among SCA patients.Additional studies should be carried out in order to investigate the mechanisms by which TGF-beta participates in the metabolism of arginase in SCA patients.

View Article: PubMed Central - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz/FIOCRUZ, Rua Waldemar Falcão 121. Brotas, Salvador, Bahia, Brazil.

ABSTRACT
Sickle cell anemia (SCA) is characterized by a marked endothelial dysfunction, owing to many factors. Arginine metabolism can be related to the inflammatory chronic state presented by patients, playing a key role in their clinical outcome and vascular endothelium. We investigated the serum arginase levels in 50 SCA patients (22 men and 28 women, mean age of 17 +/- 10.5 years) and 28 healthy controls. Serum arginase levels were associated with biochemical hemolysis markers and cytokines involved in Th17 response, as well as levels of soluble intercellular adhesion molecule-1 (sICAM-1) and vascular cell adhesion molecule-1 (sVCAM-1). Arginase concentrations were higher in SCA patients, compared with controls (p = 0.005), and were significantly and positively associated with total bilirubin (p = 0.004), indirect bilirubin (p = 0.04), and aspartate aminotransferase (AST; p = 0.039) in the SCA patient group. Moreover, arginase was significantly and positively associated with transforming growth factor-beta (TGF-beta; p = 0.008) among SCA patients. sICAM-1 was significantly and positively associated to reticulocytes (p = 0.014) and AST (p = 0.04). sVCAM-1 was likewise associated with lactate dehydrogenase (p = 0.03). These data suggest a new insight into arginase metabolism, as we show here a shift in arginine catabolism, where TGF-beta may induces the arginase pathway instead of the nitric oxide pathway and a possible involvement of the vascular activation and the serum arginase in chronic hemolysis among SCA patients. Additional studies should be carried out in order to investigate the mechanisms by which TGF-beta participates in the metabolism of arginase in SCA patients.

Show MeSH
Related in: MedlinePlus