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Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis.

Ishida W, Fukuda K, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukushima A - Mol. Vis. (2010)

Bottom Line: Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva.The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.It appears that conjunctival macrophages are APCs in the development of experimental AC.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Kochi Medical School, Nankoku-city, Japan.

ABSTRACT

Purpose: Antigen (Ag)-presenting cells (APCs) participate in the development of allergic conjunctivitis (AC). However, the conjunctival APCs that take up Ag in AC have not been identified. We sought to clarify the phenotypes of conjunctival APCs that take up, process, and present Ag to T cells during the development of experimental AC.

Methods: Splenocytes from naïve ovalbumin (OVA)-specific T cell receptor transgenic (DO11.10) mice were stimulated with either OVA, gold, or OVA-gold to evaluate stimulation-induced proliferation. Naïve DO11.10 mice were subconjunctivally injected with PBS, gold, or OVA-gold. Twenty-four hours later, conjunctivas were harvested for immunohistochemistry and electron microscopy to identify cells that engulfed OVA-gold.

Results: Stimulation of DO11.10 splenocytes with OVA-gold but not gold alone induced similar levels of proliferation as OVA alone. Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva. The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.

Conclusions: It appears that conjunctival macrophages are APCs in the development of experimental AC.

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Related in: MedlinePlus

Examination of cells that engulfed OVA-gold in the conjunctiva. Conjunctival samples were prepared as described in Figure 2. Cells that engulfed OVA-gold were examined histologically by light (A) and electron microscopy (B). Gold particles were detected in the cytoplasma of cells in the conjunctiva (A). C and R indicates conjunctiva and retina, respectively (A). Gold particles were present in cytoplasms of oval-shaped cells and spindle-like-shaped cells (B). Arrows indicate cells that engulfed gold particles.
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f3: Examination of cells that engulfed OVA-gold in the conjunctiva. Conjunctival samples were prepared as described in Figure 2. Cells that engulfed OVA-gold were examined histologically by light (A) and electron microscopy (B). Gold particles were detected in the cytoplasma of cells in the conjunctiva (A). C and R indicates conjunctiva and retina, respectively (A). Gold particles were present in cytoplasms of oval-shaped cells and spindle-like-shaped cells (B). Arrows indicate cells that engulfed gold particles.

Mentions: The above findings suggest that OVA-gold was engulfed by cells in the conjunctiva, and antigenic epitopes were presented to OVA-specific T cells. To examine this directly, conjunctivas were harvested 24 h after subconjunctival injection of OVA-gold. These conjunctivas were subjected to histological analysis by using either a light microscope or an electron microscope. Light microscopic analysis demonstrated that gold particles were detected in the cytoplasm of cells in the conjunctiva (Figure 3A). Gold particles were also detected in the extracelluar matrix spaces (data not shown). Electron microscopic analysis revealed that gold particles were present and had accumulated in the cytoplasm of oval-shaped cells and spindle-like-shaped cells (Figure 3B).


Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis.

Ishida W, Fukuda K, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukushima A - Mol. Vis. (2010)

Examination of cells that engulfed OVA-gold in the conjunctiva. Conjunctival samples were prepared as described in Figure 2. Cells that engulfed OVA-gold were examined histologically by light (A) and electron microscopy (B). Gold particles were detected in the cytoplasma of cells in the conjunctiva (A). C and R indicates conjunctiva and retina, respectively (A). Gold particles were present in cytoplasms of oval-shaped cells and spindle-like-shaped cells (B). Arrows indicate cells that engulfed gold particles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2904043&req=5

f3: Examination of cells that engulfed OVA-gold in the conjunctiva. Conjunctival samples were prepared as described in Figure 2. Cells that engulfed OVA-gold were examined histologically by light (A) and electron microscopy (B). Gold particles were detected in the cytoplasma of cells in the conjunctiva (A). C and R indicates conjunctiva and retina, respectively (A). Gold particles were present in cytoplasms of oval-shaped cells and spindle-like-shaped cells (B). Arrows indicate cells that engulfed gold particles.
Mentions: The above findings suggest that OVA-gold was engulfed by cells in the conjunctiva, and antigenic epitopes were presented to OVA-specific T cells. To examine this directly, conjunctivas were harvested 24 h after subconjunctival injection of OVA-gold. These conjunctivas were subjected to histological analysis by using either a light microscope or an electron microscope. Light microscopic analysis demonstrated that gold particles were detected in the cytoplasm of cells in the conjunctiva (Figure 3A). Gold particles were also detected in the extracelluar matrix spaces (data not shown). Electron microscopic analysis revealed that gold particles were present and had accumulated in the cytoplasm of oval-shaped cells and spindle-like-shaped cells (Figure 3B).

Bottom Line: Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva.The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.It appears that conjunctival macrophages are APCs in the development of experimental AC.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Kochi Medical School, Nankoku-city, Japan.

ABSTRACT

Purpose: Antigen (Ag)-presenting cells (APCs) participate in the development of allergic conjunctivitis (AC). However, the conjunctival APCs that take up Ag in AC have not been identified. We sought to clarify the phenotypes of conjunctival APCs that take up, process, and present Ag to T cells during the development of experimental AC.

Methods: Splenocytes from naïve ovalbumin (OVA)-specific T cell receptor transgenic (DO11.10) mice were stimulated with either OVA, gold, or OVA-gold to evaluate stimulation-induced proliferation. Naïve DO11.10 mice were subconjunctivally injected with PBS, gold, or OVA-gold. Twenty-four hours later, conjunctivas were harvested for immunohistochemistry and electron microscopy to identify cells that engulfed OVA-gold.

Results: Stimulation of DO11.10 splenocytes with OVA-gold but not gold alone induced similar levels of proliferation as OVA alone. Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva. The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.

Conclusions: It appears that conjunctival macrophages are APCs in the development of experimental AC.

Show MeSH
Related in: MedlinePlus