Limits...
Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis.

Ishida W, Fukuda K, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukushima A - Mol. Vis. (2010)

Bottom Line: Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva.The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.It appears that conjunctival macrophages are APCs in the development of experimental AC.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Kochi Medical School, Nankoku-city, Japan.

ABSTRACT

Purpose: Antigen (Ag)-presenting cells (APCs) participate in the development of allergic conjunctivitis (AC). However, the conjunctival APCs that take up Ag in AC have not been identified. We sought to clarify the phenotypes of conjunctival APCs that take up, process, and present Ag to T cells during the development of experimental AC.

Methods: Splenocytes from naïve ovalbumin (OVA)-specific T cell receptor transgenic (DO11.10) mice were stimulated with either OVA, gold, or OVA-gold to evaluate stimulation-induced proliferation. Naïve DO11.10 mice were subconjunctivally injected with PBS, gold, or OVA-gold. Twenty-four hours later, conjunctivas were harvested for immunohistochemistry and electron microscopy to identify cells that engulfed OVA-gold.

Results: Stimulation of DO11.10 splenocytes with OVA-gold but not gold alone induced similar levels of proliferation as OVA alone. Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva. The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.

Conclusions: It appears that conjunctival macrophages are APCs in the development of experimental AC.

Show MeSH

Related in: MedlinePlus

Effect of subconjunctival injection of OVA-gold in the conjunctiva. OVA-gold, gold or PBS (10 μl) was injected into the subconjunctival space of DO11.10 mice. Twenty-four hours later, conjunctivas were collected for immunohistochemical analyses. MBP-positive eosinophils and CD4-positive T cells were detected in the OVA-gold-injected group (A). Control samples were not stained with any primary antibodies. MBP-positive eosinophils were quantified in the OVA-gold and control groups (B). The double asterisk indicates a p<0.01 compared to PBS and the gold groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2904043&req=5

f2: Effect of subconjunctival injection of OVA-gold in the conjunctiva. OVA-gold, gold or PBS (10 μl) was injected into the subconjunctival space of DO11.10 mice. Twenty-four hours later, conjunctivas were collected for immunohistochemical analyses. MBP-positive eosinophils and CD4-positive T cells were detected in the OVA-gold-injected group (A). Control samples were not stained with any primary antibodies. MBP-positive eosinophils were quantified in the OVA-gold and control groups (B). The double asterisk indicates a p<0.01 compared to PBS and the gold groups.

Mentions: Next, we investigated whether OVA-gold has antigenicity in vivo. To this end, OVA-gold was injected into the subconjunctival space of DO11.10 mice. Subconjunctival injection of OVA-gold induced an influx of inflammatory cells in the conjunctiva and immunohistochemical analysis of conjunctival infiltrating cells revealed that MBP-positive eosinophils and CD4-positive T cells infiltrated into the conjunctiva of OVA-gold-injected mice (Figure 2A). Subconjunctival injection of gold alone or PBS did not induce apparent inflammation in the conjunctiva (data not shown). Counting of MBP-positive eosinophils revealed that significantly more eosinophils were detected in the conjunctiva of the OVA-gold-injected group compared to the gold alone- or PBS-injected groups (Figure 2B). These results indicate that OVA-gold has antigenicity in vivo as well as in vitro.


Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis.

Ishida W, Fukuda K, Kajisako M, Takahashi A, Sumi T, van Rooijen N, Fukushima A - Mol. Vis. (2010)

Effect of subconjunctival injection of OVA-gold in the conjunctiva. OVA-gold, gold or PBS (10 μl) was injected into the subconjunctival space of DO11.10 mice. Twenty-four hours later, conjunctivas were collected for immunohistochemical analyses. MBP-positive eosinophils and CD4-positive T cells were detected in the OVA-gold-injected group (A). Control samples were not stained with any primary antibodies. MBP-positive eosinophils were quantified in the OVA-gold and control groups (B). The double asterisk indicates a p<0.01 compared to PBS and the gold groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2904043&req=5

f2: Effect of subconjunctival injection of OVA-gold in the conjunctiva. OVA-gold, gold or PBS (10 μl) was injected into the subconjunctival space of DO11.10 mice. Twenty-four hours later, conjunctivas were collected for immunohistochemical analyses. MBP-positive eosinophils and CD4-positive T cells were detected in the OVA-gold-injected group (A). Control samples were not stained with any primary antibodies. MBP-positive eosinophils were quantified in the OVA-gold and control groups (B). The double asterisk indicates a p<0.01 compared to PBS and the gold groups.
Mentions: Next, we investigated whether OVA-gold has antigenicity in vivo. To this end, OVA-gold was injected into the subconjunctival space of DO11.10 mice. Subconjunctival injection of OVA-gold induced an influx of inflammatory cells in the conjunctiva and immunohistochemical analysis of conjunctival infiltrating cells revealed that MBP-positive eosinophils and CD4-positive T cells infiltrated into the conjunctiva of OVA-gold-injected mice (Figure 2A). Subconjunctival injection of gold alone or PBS did not induce apparent inflammation in the conjunctiva (data not shown). Counting of MBP-positive eosinophils revealed that significantly more eosinophils were detected in the conjunctiva of the OVA-gold-injected group compared to the gold alone- or PBS-injected groups (Figure 2B). These results indicate that OVA-gold has antigenicity in vivo as well as in vitro.

Bottom Line: Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva.The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.It appears that conjunctival macrophages are APCs in the development of experimental AC.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Kochi Medical School, Nankoku-city, Japan.

ABSTRACT

Purpose: Antigen (Ag)-presenting cells (APCs) participate in the development of allergic conjunctivitis (AC). However, the conjunctival APCs that take up Ag in AC have not been identified. We sought to clarify the phenotypes of conjunctival APCs that take up, process, and present Ag to T cells during the development of experimental AC.

Methods: Splenocytes from naïve ovalbumin (OVA)-specific T cell receptor transgenic (DO11.10) mice were stimulated with either OVA, gold, or OVA-gold to evaluate stimulation-induced proliferation. Naïve DO11.10 mice were subconjunctivally injected with PBS, gold, or OVA-gold. Twenty-four hours later, conjunctivas were harvested for immunohistochemistry and electron microscopy to identify cells that engulfed OVA-gold.

Results: Stimulation of DO11.10 splenocytes with OVA-gold but not gold alone induced similar levels of proliferation as OVA alone. Subconjunctival injection of OVA-gold, but not gold alone, induced infiltration of eosinophils and CD4-positive T cells into the conjunctiva. The cells that took up OVA-gold into their cytoplasma expressed Cluster of Differentiation (CD) 11b, CD68, major histocompatibility complex (MHC) class II.

Conclusions: It appears that conjunctival macrophages are APCs in the development of experimental AC.

Show MeSH
Related in: MedlinePlus