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Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium.

Posadas SJ, Caz V, Caballero I, Cendejas E, Quilez I, Largo C, Elvira M, De Miguel E - BMC Gastroenterol (2010)

Bottom Line: Curiosly, the mannoprotein effect was dose dependent.Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear.These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Surgery Department, La Paz Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain.

ABSTRACT

Background: Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria.This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium.

Methods: Adult Wistar male rats were divided into three groups: control, and mannoprotein E1 at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E1. Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry.

Results: Ininfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent.

Conclusions: Mannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

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Related in: MedlinePlus

Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in colon samples. Figure 3A and 3B illustrate in a bar diagram that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein (n = 9 control group, n = 8 mannoprotein group). Figure 3C shows IL-1β mRNA expression in both groups represented as a bar diagram. No statistical differences were observed between both groups. Finally figure 3D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p < 0.05 compared with control.
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Figure 3: Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in colon samples. Figure 3A and 3B illustrate in a bar diagram that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein (n = 9 control group, n = 8 mannoprotein group). Figure 3C shows IL-1β mRNA expression in both groups represented as a bar diagram. No statistical differences were observed between both groups. Finally figure 3D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p < 0.05 compared with control.

Mentions: Proinflammatory cytokine mRNA expression and TLR5 levels were similar in colon to levels in jejunum with the controls showing higher IL-6 levels than the mannopprotein-treated animals (p < 0.001; figure 3A). TNF-alpha and IL-1beta levels were higher in the control group than the mannoprotein-treated group (p < 0.001) in both analyses (figure 3B and 3C). TLR5 gene transcription levels also showed statistical differences between the two groups. p < 0.001; figure 3D.


Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium.

Posadas SJ, Caz V, Caballero I, Cendejas E, Quilez I, Largo C, Elvira M, De Miguel E - BMC Gastroenterol (2010)

Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in colon samples. Figure 3A and 3B illustrate in a bar diagram that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein (n = 9 control group, n = 8 mannoprotein group). Figure 3C shows IL-1β mRNA expression in both groups represented as a bar diagram. No statistical differences were observed between both groups. Finally figure 3D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p < 0.05 compared with control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2903505&req=5

Figure 3: Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in colon samples. Figure 3A and 3B illustrate in a bar diagram that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein (n = 9 control group, n = 8 mannoprotein group). Figure 3C shows IL-1β mRNA expression in both groups represented as a bar diagram. No statistical differences were observed between both groups. Finally figure 3D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p < 0.05 compared with control.
Mentions: Proinflammatory cytokine mRNA expression and TLR5 levels were similar in colon to levels in jejunum with the controls showing higher IL-6 levels than the mannopprotein-treated animals (p < 0.001; figure 3A). TNF-alpha and IL-1beta levels were higher in the control group than the mannoprotein-treated group (p < 0.001) in both analyses (figure 3B and 3C). TLR5 gene transcription levels also showed statistical differences between the two groups. p < 0.001; figure 3D.

Bottom Line: Curiosly, the mannoprotein effect was dose dependent.Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear.These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Surgery Department, La Paz Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain.

ABSTRACT

Background: Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria.This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium.

Methods: Adult Wistar male rats were divided into three groups: control, and mannoprotein E1 at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E1. Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry.

Results: Ininfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent.

Conclusions: Mannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

Show MeSH
Related in: MedlinePlus