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Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium.

Posadas SJ, Caz V, Caballero I, Cendejas E, Quilez I, Largo C, Elvira M, De Miguel E - BMC Gastroenterol (2010)

Bottom Line: Curiosly, the mannoprotein effect was dose dependent.Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear.These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Surgery Department, La Paz Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain.

ABSTRACT

Background: Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria.This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium.

Methods: Adult Wistar male rats were divided into three groups: control, and mannoprotein E1 at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E1. Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry.

Results: Ininfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent.

Conclusions: Mannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

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Related in: MedlinePlus

Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in jejune samples. Figure 1A and 1B illustrate in a bar diagrams that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein. Figure 1C shows IL-1β mRNA expression in both groups represented as bars diagram (n = 9 control group, n = 8 mannoprotein group). No statistical differences were observed between both groups. Finally, figure 1D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p > 0.05 compared with control.
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Figure 1: Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in jejune samples. Figure 1A and 1B illustrate in a bar diagrams that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein. Figure 1C shows IL-1β mRNA expression in both groups represented as bars diagram (n = 9 control group, n = 8 mannoprotein group). No statistical differences were observed between both groups. Finally, figure 1D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p > 0.05 compared with control.

Mentions: Figures 1A,B,C and 1D illustrate the results for IL-6, TNF-alpha, IL-1beta and TLR5 levels respectively as a ratio of mRNA expression compared with the expression of the S26 gene, which was employed as a housekeeping gene. IL-6 levels were higher in the control group than in the mannoprotein-treated animals (p = 0.001; figure 1A). Levels of TNF-alpha and IL-1beta were also higher in the control group than the mannoprotein treated animal group (p = 0.001) in both analyses. Levels of TLR5 mRNA expression also differed significantly between groups (p < 0.001, figure 1D).


Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium.

Posadas SJ, Caz V, Caballero I, Cendejas E, Quilez I, Largo C, Elvira M, De Miguel E - BMC Gastroenterol (2010)

Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in jejune samples. Figure 1A and 1B illustrate in a bar diagrams that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein. Figure 1C shows IL-1β mRNA expression in both groups represented as bars diagram (n = 9 control group, n = 8 mannoprotein group). No statistical differences were observed between both groups. Finally, figure 1D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p > 0.05 compared with control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2903505&req=5

Figure 1: Quantification of IL-6, TNF-α, IL-1β and TLR5 mRNA in jejune samples. Figure 1A and 1B illustrate in a bar diagrams that IL-6 and TNF-α mRNA expression increased markedly in control group compared with mannoprotein. Figure 1C shows IL-1β mRNA expression in both groups represented as bars diagram (n = 9 control group, n = 8 mannoprotein group). No statistical differences were observed between both groups. Finally, figure 1D shows the results for TLR5 gene expression in both groups with augmented levels in the control group compared with mannoprotein. Error bars represent the standard deviations. * Significant at p > 0.05 compared with control.
Mentions: Figures 1A,B,C and 1D illustrate the results for IL-6, TNF-alpha, IL-1beta and TLR5 levels respectively as a ratio of mRNA expression compared with the expression of the S26 gene, which was employed as a housekeeping gene. IL-6 levels were higher in the control group than in the mannoprotein-treated animals (p = 0.001; figure 1A). Levels of TNF-alpha and IL-1beta were also higher in the control group than the mannoprotein treated animal group (p = 0.001) in both analyses. Levels of TLR5 mRNA expression also differed significantly between groups (p < 0.001, figure 1D).

Bottom Line: Curiosly, the mannoprotein effect was dose dependent.Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear.These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Surgery Department, La Paz Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain.

ABSTRACT

Background: Mannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria.This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium.

Methods: Adult Wistar male rats were divided into three groups: control, and mannoprotein E1 at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E1. Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry.

Results: Ininfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent.

Conclusions: Mannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

Show MeSH
Related in: MedlinePlus