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Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis.

Huss S, Schmitz J, Goltz D, Fischer HP, Büttner R, Weiskirchen R - Fibrogenesis Tissue Repair (2010)

Bottom Line: The results were compared to a semi-quantitative scoring system.Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter.During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pathology, University of Bonn, Bonn, Germany. sebastian.huss@ukb.uni-bonn.de.

ABSTRACT

Background: Computer-based morphometry can minimize subjectivity in the assessment of liver fibrosis. An image processing program was developed with Delphi for the quantification of fibrosis in liver tissue samples stained with Sirius Red. Bile duct ligated and sham operated wild type C57BL/6 mice served as a model of time-dependent induction of liver fibrosis. Formation of fibrosis was determined with the developed software at day 0, 3, 7, 10, 14, 20, 30 and 60. The results were compared to a semi-quantitative scoring system.

Results: Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter. During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin. The results from our computer-based morphometric analysis were highly correlated with the results that were obtained in a standardized pathology semi-quantitative scoring system (R 2 = 0.89, n = 38).

Conclusions: Using our Delphi-based image analysing software, the morphometric assessment of fibrosis is as precise as semi-quantitative scoring by an experienced pathologist. This program can be a valuable tool in any kind of experimental or clinical setting for standardized quantitative assessment of fibrosis.

No MeSH data available.


Related in: MedlinePlus

Serum markers of cholestasis and hepatocellular injury during bile duct ligation. Biochemical markers are shown for (A) bilirubin, (B) total protein, (C) alanine aminotransferase and (D) aspartate transaminase.
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Figure 2: Serum markers of cholestasis and hepatocellular injury during bile duct ligation. Biochemical markers are shown for (A) bilirubin, (B) total protein, (C) alanine aminotransferase and (D) aspartate transaminase.

Mentions: Alanine aminotransferase (ALT) and aspartate transaminase (AST) increased rapidly after BDL, peaking at 7 respectively twenty days after the surgery. After the peaking, ALT and AST decreased steadily until day 30; serum levels remained almost unchanged after 60 days. Bilirubin levels steadily elevated and reached a plateau after 7 days. Total protein serum levels had a greater variability with a slight decrease after 7 days (Figure 2).


Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis.

Huss S, Schmitz J, Goltz D, Fischer HP, Büttner R, Weiskirchen R - Fibrogenesis Tissue Repair (2010)

Serum markers of cholestasis and hepatocellular injury during bile duct ligation. Biochemical markers are shown for (A) bilirubin, (B) total protein, (C) alanine aminotransferase and (D) aspartate transaminase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2903497&req=5

Figure 2: Serum markers of cholestasis and hepatocellular injury during bile duct ligation. Biochemical markers are shown for (A) bilirubin, (B) total protein, (C) alanine aminotransferase and (D) aspartate transaminase.
Mentions: Alanine aminotransferase (ALT) and aspartate transaminase (AST) increased rapidly after BDL, peaking at 7 respectively twenty days after the surgery. After the peaking, ALT and AST decreased steadily until day 30; serum levels remained almost unchanged after 60 days. Bilirubin levels steadily elevated and reached a plateau after 7 days. Total protein serum levels had a greater variability with a slight decrease after 7 days (Figure 2).

Bottom Line: The results were compared to a semi-quantitative scoring system.Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter.During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pathology, University of Bonn, Bonn, Germany. sebastian.huss@ukb.uni-bonn.de.

ABSTRACT

Background: Computer-based morphometry can minimize subjectivity in the assessment of liver fibrosis. An image processing program was developed with Delphi for the quantification of fibrosis in liver tissue samples stained with Sirius Red. Bile duct ligated and sham operated wild type C57BL/6 mice served as a model of time-dependent induction of liver fibrosis. Formation of fibrosis was determined with the developed software at day 0, 3, 7, 10, 14, 20, 30 and 60. The results were compared to a semi-quantitative scoring system.

Results: Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter. During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin. The results from our computer-based morphometric analysis were highly correlated with the results that were obtained in a standardized pathology semi-quantitative scoring system (R 2 = 0.89, n = 38).

Conclusions: Using our Delphi-based image analysing software, the morphometric assessment of fibrosis is as precise as semi-quantitative scoring by an experienced pathologist. This program can be a valuable tool in any kind of experimental or clinical setting for standardized quantitative assessment of fibrosis.

No MeSH data available.


Related in: MedlinePlus