Limits...
Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis.

Huss S, Schmitz J, Goltz D, Fischer HP, Büttner R, Weiskirchen R - Fibrogenesis Tissue Repair (2010)

Bottom Line: The results were compared to a semi-quantitative scoring system.Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter.During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pathology, University of Bonn, Bonn, Germany. sebastian.huss@ukb.uni-bonn.de.

ABSTRACT

Background: Computer-based morphometry can minimize subjectivity in the assessment of liver fibrosis. An image processing program was developed with Delphi for the quantification of fibrosis in liver tissue samples stained with Sirius Red. Bile duct ligated and sham operated wild type C57BL/6 mice served as a model of time-dependent induction of liver fibrosis. Formation of fibrosis was determined with the developed software at day 0, 3, 7, 10, 14, 20, 30 and 60. The results were compared to a semi-quantitative scoring system.

Results: Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter. During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin. The results from our computer-based morphometric analysis were highly correlated with the results that were obtained in a standardized pathology semi-quantitative scoring system (R 2 = 0.89, n = 38).

Conclusions: Using our Delphi-based image analysing software, the morphometric assessment of fibrosis is as precise as semi-quantitative scoring by an experienced pathologist. This program can be a valuable tool in any kind of experimental or clinical setting for standardized quantitative assessment of fibrosis.

No MeSH data available.


Related in: MedlinePlus

Study design and experimental setting. Bile duct ligation was performed on day 0. Analysis was performed on day 3 and 7 after the procedure to measure short time effects. Time-points after 10, 14 and 20 days were chosen to display intermediate and 30 and 60 days to analyse long-term effects.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2903497&req=5

Figure 1: Study design and experimental setting. Bile duct ligation was performed on day 0. Analysis was performed on day 3 and 7 after the procedure to measure short time effects. Time-points after 10, 14 and 20 days were chosen to display intermediate and 30 and 60 days to analyse long-term effects.

Mentions: We have performed BDL in 40 animals and analysed ongoing hepatic fibrogenesis in respective animals at fixed time points (Figure 1). All sham-operated animals survived but two of 40 mice in the BDL group became moribund and were killed before the planned end point. The activity of sham-operated animals was nearly normal 24 h after the lapratomy while the animals subjected to BDL showed reduced activity during the first 72 h but regained normal activity thereafter. Jaundiced skin was already apparent in all animals 24 h after BDL.


Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis.

Huss S, Schmitz J, Goltz D, Fischer HP, Büttner R, Weiskirchen R - Fibrogenesis Tissue Repair (2010)

Study design and experimental setting. Bile duct ligation was performed on day 0. Analysis was performed on day 3 and 7 after the procedure to measure short time effects. Time-points after 10, 14 and 20 days were chosen to display intermediate and 30 and 60 days to analyse long-term effects.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2903497&req=5

Figure 1: Study design and experimental setting. Bile duct ligation was performed on day 0. Analysis was performed on day 3 and 7 after the procedure to measure short time effects. Time-points after 10, 14 and 20 days were chosen to display intermediate and 30 and 60 days to analyse long-term effects.
Mentions: We have performed BDL in 40 animals and analysed ongoing hepatic fibrogenesis in respective animals at fixed time points (Figure 1). All sham-operated animals survived but two of 40 mice in the BDL group became moribund and were killed before the planned end point. The activity of sham-operated animals was nearly normal 24 h after the lapratomy while the animals subjected to BDL showed reduced activity during the first 72 h but regained normal activity thereafter. Jaundiced skin was already apparent in all animals 24 h after BDL.

Bottom Line: The results were compared to a semi-quantitative scoring system.Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter.During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pathology, University of Bonn, Bonn, Germany. sebastian.huss@ukb.uni-bonn.de.

ABSTRACT

Background: Computer-based morphometry can minimize subjectivity in the assessment of liver fibrosis. An image processing program was developed with Delphi for the quantification of fibrosis in liver tissue samples stained with Sirius Red. Bile duct ligated and sham operated wild type C57BL/6 mice served as a model of time-dependent induction of liver fibrosis. Formation of fibrosis was determined with the developed software at day 0, 3, 7, 10, 14, 20, 30 and 60. The results were compared to a semi-quantitative scoring system.

Results: Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter. During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin. The results from our computer-based morphometric analysis were highly correlated with the results that were obtained in a standardized pathology semi-quantitative scoring system (R 2 = 0.89, n = 38).

Conclusions: Using our Delphi-based image analysing software, the morphometric assessment of fibrosis is as precise as semi-quantitative scoring by an experienced pathologist. This program can be a valuable tool in any kind of experimental or clinical setting for standardized quantitative assessment of fibrosis.

No MeSH data available.


Related in: MedlinePlus