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Biological effects of rinsing morsellised bone grafts before and after impaction.

Hannink G, Piek E, Hendriks JM, Van der Kraan PM, Schreurs BW, Buma P - Int Orthop (2008)

Bottom Line: The fluid was analysed for the presence of TGF-betas and BMPs, and the osteoinductive capacity was tested on hMSCs.Abundant TGF-beta was present in the fluid.Osteogenic differentiation of hMSCs was inhibited by the fluid.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.

ABSTRACT
Rinsing bone grafts before or both before and after impaction might have different effects on the incorporation of the graft. Rinsing again after impaction might negatively influence bone induction if growth factors released by impaction are washed away. We studied if transforming growth factor-betas (TGF-betas) and bone morphogenetic proteins (BMPs) are released from the mineralised matrix by impaction and if these released growth factors induce osteogenic differentiation in human mesenchymal stem cells (hMSCs). Rinsed morsellised bone allografts were impacted in a cylinder and the escaping fluid was collected. The fluid was analysed for the presence of TGF-betas and BMPs, and the osteoinductive capacity was tested on hMSCs. Abundant TGF-beta was present in the fluid. No BMPs could be detected. Osteogenic differentiation of hMSCs was inhibited by the fluid. Results from our study leave us only able to speculate whether rinsing grafts again after impaction has a beneficial effect on the incorporation process or not.

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Related in: MedlinePlus

Level of transforming growth factor-β1 and -β2 (TGF-β1 and TGF-β2) measured in the “impaction fluid.” Both total and already active growth factor levels are shown. Data are mean±standard deviation (SD) of experiments performed in triplo
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Fig1: Level of transforming growth factor-β1 and -β2 (TGF-β1 and TGF-β2) measured in the “impaction fluid.” Both total and already active growth factor levels are shown. Data are mean±standard deviation (SD) of experiments performed in triplo

Mentions: Analysis of the “impaction fluid” revealed that TGF-β was abundant. Both TGF-β1 and TGF-β2 were present in the “impaction fluid” in physiologically significant amounts. The total levels of TGF-β1 and TGF-β2 present after activation were 5.39 (±1.12) ng/g bone and 1.20 (±0.12) ng/g bone, respectively. Before the samples were activated, an already active amount of 0.43 (±0.05) ng TGF-β1/g bone and 0.13 (±0.11) ng TGF-β2/g bone was measured (Fig. 1). No BMPs could be detected using the BMP-selective reporter bioassay. Luciferase expression did not exceed the detection limit of 1.6 ng BMP-2/ml.Fig. 1


Biological effects of rinsing morsellised bone grafts before and after impaction.

Hannink G, Piek E, Hendriks JM, Van der Kraan PM, Schreurs BW, Buma P - Int Orthop (2008)

Level of transforming growth factor-β1 and -β2 (TGF-β1 and TGF-β2) measured in the “impaction fluid.” Both total and already active growth factor levels are shown. Data are mean±standard deviation (SD) of experiments performed in triplo
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2903101&req=5

Fig1: Level of transforming growth factor-β1 and -β2 (TGF-β1 and TGF-β2) measured in the “impaction fluid.” Both total and already active growth factor levels are shown. Data are mean±standard deviation (SD) of experiments performed in triplo
Mentions: Analysis of the “impaction fluid” revealed that TGF-β was abundant. Both TGF-β1 and TGF-β2 were present in the “impaction fluid” in physiologically significant amounts. The total levels of TGF-β1 and TGF-β2 present after activation were 5.39 (±1.12) ng/g bone and 1.20 (±0.12) ng/g bone, respectively. Before the samples were activated, an already active amount of 0.43 (±0.05) ng TGF-β1/g bone and 0.13 (±0.11) ng TGF-β2/g bone was measured (Fig. 1). No BMPs could be detected using the BMP-selective reporter bioassay. Luciferase expression did not exceed the detection limit of 1.6 ng BMP-2/ml.Fig. 1

Bottom Line: The fluid was analysed for the presence of TGF-betas and BMPs, and the osteoinductive capacity was tested on hMSCs.Abundant TGF-beta was present in the fluid.Osteogenic differentiation of hMSCs was inhibited by the fluid.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.

ABSTRACT
Rinsing bone grafts before or both before and after impaction might have different effects on the incorporation of the graft. Rinsing again after impaction might negatively influence bone induction if growth factors released by impaction are washed away. We studied if transforming growth factor-betas (TGF-betas) and bone morphogenetic proteins (BMPs) are released from the mineralised matrix by impaction and if these released growth factors induce osteogenic differentiation in human mesenchymal stem cells (hMSCs). Rinsed morsellised bone allografts were impacted in a cylinder and the escaping fluid was collected. The fluid was analysed for the presence of TGF-betas and BMPs, and the osteoinductive capacity was tested on hMSCs. Abundant TGF-beta was present in the fluid. No BMPs could be detected. Osteogenic differentiation of hMSCs was inhibited by the fluid. Results from our study leave us only able to speculate whether rinsing grafts again after impaction has a beneficial effect on the incorporation process or not.

Show MeSH
Related in: MedlinePlus