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Significant Growth Inhibition of Canine Mammary Carcinoma Xenografts following Treatment with Oncolytic Vaccinia Virus GLV-1h68.

Gentschev I, Ehrig K, Donat U, Hess M, Rudolph S, Chen N, Yu YA, Zhang Q, Bullerdiek J, Nolte I, Stritzker J, Szalay AA - J Oncol (2010)

Bottom Line: Therefore, there is an urgent need to identify novel agents for therapy of this disease.Finally, infection with GLV-1h68 led to strong inflammatory and oncolytic effects resulting in significant growth inhibition of the tumors.In summary, the data showed that the GLV-1h68 virus strain has promising potential for effective treatment of canine mammary carcinoma.

View Article: PubMed Central - PubMed

Affiliation: Genelux Corporation, San Diego Science Center, San Diego, CA 92109, USA.

ABSTRACT
Canine mammary carcinoma is a highly metastatic tumor that is poorly responsive to available treatment. Therefore, there is an urgent need to identify novel agents for therapy of this disease. Recently, we reported that the oncolytic vaccinia virus GLV-1h68 could be a useful tool for therapy of canine mammary adenoma in vivo. In this study we analyzed the therapeutic effect of GLV-1h68 against canine mammary carcinoma. Cell culture data demonstrated that GLV-1h68 efficiently infected and destroyed cells of the mammary carcinoma cell line MTH52c. Furthermore, after systemic administration, this attenuated vaccinia virus strain primarily replicated in canine tumor xenografts in nude mice. Finally, infection with GLV-1h68 led to strong inflammatory and oncolytic effects resulting in significant growth inhibition of the tumors. In summary, the data showed that the GLV-1h68 virus strain has promising potential for effective treatment of canine mammary carcinoma.

No MeSH data available.


Related in: MedlinePlus

Time-dependent effects of infection of MTH52c with GLV-1h68 at an MOI of 1.0. (BF) Transmitted light view of virus-infected MTH52c cells; (GFP) expression of GFP in infected cells detected by direct fluorescence; (PI) propidium iodide staining of dead cells; (Merged) colocalization of GFP with the dead cells is shown in the merged imaged. All pictures in this set were taken at the same magnification. Scale bars represent 0.1 mm.
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fig3: Time-dependent effects of infection of MTH52c with GLV-1h68 at an MOI of 1.0. (BF) Transmitted light view of virus-infected MTH52c cells; (GFP) expression of GFP in infected cells detected by direct fluorescence; (PI) propidium iodide staining of dead cells; (Merged) colocalization of GFP with the dead cells is shown in the merged imaged. All pictures in this set were taken at the same magnification. Scale bars represent 0.1 mm.

Mentions: Similar data were obtained by fluorescence microscopy (Figure 3). In these experimental settings we found that MTH52c cells infected with GLV-1h68 at MOI of 0.1 and 1.0 exhibited the strongest GFP expression at 72 and 96 hours, respectively, (supplementary Figure 1 and Figure 3). These data were also confirmed by flow cytometry (Figure 4) showing that the amount of infected cells increased over time and that those cells infected with vaccinia virus (detectable by GFP expression) were those cells that exhibited the major population of dead/dying cells (detectable by positive propidium iodide staining). In fluorescence microscopy, we used the same dye to demonstrate that most of the infected cells were dead/dying at 96h p.i. (Figure 3). These results indicate that GLV-1h68 was able to efficiently infect, replicate, and kill the MTH52c cells in cell culture.


Significant Growth Inhibition of Canine Mammary Carcinoma Xenografts following Treatment with Oncolytic Vaccinia Virus GLV-1h68.

Gentschev I, Ehrig K, Donat U, Hess M, Rudolph S, Chen N, Yu YA, Zhang Q, Bullerdiek J, Nolte I, Stritzker J, Szalay AA - J Oncol (2010)

Time-dependent effects of infection of MTH52c with GLV-1h68 at an MOI of 1.0. (BF) Transmitted light view of virus-infected MTH52c cells; (GFP) expression of GFP in infected cells detected by direct fluorescence; (PI) propidium iodide staining of dead cells; (Merged) colocalization of GFP with the dead cells is shown in the merged imaged. All pictures in this set were taken at the same magnification. Scale bars represent 0.1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2902752&req=5

fig3: Time-dependent effects of infection of MTH52c with GLV-1h68 at an MOI of 1.0. (BF) Transmitted light view of virus-infected MTH52c cells; (GFP) expression of GFP in infected cells detected by direct fluorescence; (PI) propidium iodide staining of dead cells; (Merged) colocalization of GFP with the dead cells is shown in the merged imaged. All pictures in this set were taken at the same magnification. Scale bars represent 0.1 mm.
Mentions: Similar data were obtained by fluorescence microscopy (Figure 3). In these experimental settings we found that MTH52c cells infected with GLV-1h68 at MOI of 0.1 and 1.0 exhibited the strongest GFP expression at 72 and 96 hours, respectively, (supplementary Figure 1 and Figure 3). These data were also confirmed by flow cytometry (Figure 4) showing that the amount of infected cells increased over time and that those cells infected with vaccinia virus (detectable by GFP expression) were those cells that exhibited the major population of dead/dying cells (detectable by positive propidium iodide staining). In fluorescence microscopy, we used the same dye to demonstrate that most of the infected cells were dead/dying at 96h p.i. (Figure 3). These results indicate that GLV-1h68 was able to efficiently infect, replicate, and kill the MTH52c cells in cell culture.

Bottom Line: Therefore, there is an urgent need to identify novel agents for therapy of this disease.Finally, infection with GLV-1h68 led to strong inflammatory and oncolytic effects resulting in significant growth inhibition of the tumors.In summary, the data showed that the GLV-1h68 virus strain has promising potential for effective treatment of canine mammary carcinoma.

View Article: PubMed Central - PubMed

Affiliation: Genelux Corporation, San Diego Science Center, San Diego, CA 92109, USA.

ABSTRACT
Canine mammary carcinoma is a highly metastatic tumor that is poorly responsive to available treatment. Therefore, there is an urgent need to identify novel agents for therapy of this disease. Recently, we reported that the oncolytic vaccinia virus GLV-1h68 could be a useful tool for therapy of canine mammary adenoma in vivo. In this study we analyzed the therapeutic effect of GLV-1h68 against canine mammary carcinoma. Cell culture data demonstrated that GLV-1h68 efficiently infected and destroyed cells of the mammary carcinoma cell line MTH52c. Furthermore, after systemic administration, this attenuated vaccinia virus strain primarily replicated in canine tumor xenografts in nude mice. Finally, infection with GLV-1h68 led to strong inflammatory and oncolytic effects resulting in significant growth inhibition of the tumors. In summary, the data showed that the GLV-1h68 virus strain has promising potential for effective treatment of canine mammary carcinoma.

No MeSH data available.


Related in: MedlinePlus