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A combined transcriptomics and lipidomics analysis of subcutaneous, epididymal and mesenteric adipose tissue reveals marked functional differences.

Caesar R, Manieri M, Kelder T, Boekschoten M, Evelo C, Müller M, Kooistra T, Cinti S, Kleemann R, Drevon CA - PLoS ONE (2010)

Bottom Line: EWAT was found to exhibit physiological zonation.The contents of linoleic acid and alpha-linolenic acid in EWAT were increased compared to other depots.We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and alpha-linolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway. Robert.Caesar@wlab.gu.se

ABSTRACT
Depot-dependent differences in adipose tissue physiology may reflect specialized functions and local interactions between adipocytes and surrounding tissues. We combined time-resolved microarray analyses of mesenteric- (MWAT), subcutaneous- (SWAT) and epididymal adipose tissue (EWAT) during high-fat feeding of male transgenic ApoE3Leiden mice with histology, targeted lipidomics and biochemical analyses of metabolic pathways to identify differentially regulated processes and site-specific functions. EWAT was found to exhibit physiological zonation. De novo lipogenesis in fat proximal to epididymis was stably low, whereas de novo lipogenesis distal to epididymis and at other locations was down-regulated in response to high-fat diet. The contents of linoleic acid and alpha-linolenic acid in EWAT were increased compared to other depots. Expression of the androgen receptor (Ar) was higher in EWAT than in MWAT and SWAT. We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and alpha-linolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis.

Show MeSH
Location of the proximal and distal section of the epididymal adipose tissue (EWAT).Adipose tissue at the distal end of the depot, limited by the upper dashed line, is denoted distal adipose tissue. Adipose tissue associated with the head and the tail of epididymis, indicated by the lower dashed line, is denoted proximal adipose tissue.
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pone-0011525-g001: Location of the proximal and distal section of the epididymal adipose tissue (EWAT).Adipose tissue at the distal end of the depot, limited by the upper dashed line, is denoted distal adipose tissue. Adipose tissue associated with the head and the tail of epididymis, indicated by the lower dashed line, is denoted proximal adipose tissue.

Mentions: Another study was performed for RT-PCR, lipidomics analyses and radioactive tracer experiments. Male ApoE3L mice were housed in groups of 2–3 animals and fed standard chow (4.2% fat (w/w; 0.7% saturated, 1.2% MUFA and 1.6% PUFA), 22.3% protein, 33.9% starch and 5.7% sugars) (Beekay feeds, B & K Universal Ltd., Nittedal, Norway) and water ad libitum. Mice were sacrificed at 12–14 weeks of age. The animals were fasted for 5 hours before being euthanized by cervical dislocation at 13.00. Mesenteric and inguinal subcutaneous adipose tissue (whole depots) and epididymal adipose tissue located within 10 mm from the epididymis (proximal) and within 10 mm from the distal end of the epididymal fat depot (distal) were harvested (Figure 1). Fat samples were snap-frozen immediately in liquid nitrogen, and stored at −80°C until analyses, except for some samples immediately used for ex vivo analysis of de novo lipogenesis and fatty acid accumulation in tissue explants.


A combined transcriptomics and lipidomics analysis of subcutaneous, epididymal and mesenteric adipose tissue reveals marked functional differences.

Caesar R, Manieri M, Kelder T, Boekschoten M, Evelo C, Müller M, Kooistra T, Cinti S, Kleemann R, Drevon CA - PLoS ONE (2010)

Location of the proximal and distal section of the epididymal adipose tissue (EWAT).Adipose tissue at the distal end of the depot, limited by the upper dashed line, is denoted distal adipose tissue. Adipose tissue associated with the head and the tail of epididymis, indicated by the lower dashed line, is denoted proximal adipose tissue.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2902507&req=5

pone-0011525-g001: Location of the proximal and distal section of the epididymal adipose tissue (EWAT).Adipose tissue at the distal end of the depot, limited by the upper dashed line, is denoted distal adipose tissue. Adipose tissue associated with the head and the tail of epididymis, indicated by the lower dashed line, is denoted proximal adipose tissue.
Mentions: Another study was performed for RT-PCR, lipidomics analyses and radioactive tracer experiments. Male ApoE3L mice were housed in groups of 2–3 animals and fed standard chow (4.2% fat (w/w; 0.7% saturated, 1.2% MUFA and 1.6% PUFA), 22.3% protein, 33.9% starch and 5.7% sugars) (Beekay feeds, B & K Universal Ltd., Nittedal, Norway) and water ad libitum. Mice were sacrificed at 12–14 weeks of age. The animals were fasted for 5 hours before being euthanized by cervical dislocation at 13.00. Mesenteric and inguinal subcutaneous adipose tissue (whole depots) and epididymal adipose tissue located within 10 mm from the epididymis (proximal) and within 10 mm from the distal end of the epididymal fat depot (distal) were harvested (Figure 1). Fat samples were snap-frozen immediately in liquid nitrogen, and stored at −80°C until analyses, except for some samples immediately used for ex vivo analysis of de novo lipogenesis and fatty acid accumulation in tissue explants.

Bottom Line: EWAT was found to exhibit physiological zonation.The contents of linoleic acid and alpha-linolenic acid in EWAT were increased compared to other depots.We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and alpha-linolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway. Robert.Caesar@wlab.gu.se

ABSTRACT
Depot-dependent differences in adipose tissue physiology may reflect specialized functions and local interactions between adipocytes and surrounding tissues. We combined time-resolved microarray analyses of mesenteric- (MWAT), subcutaneous- (SWAT) and epididymal adipose tissue (EWAT) during high-fat feeding of male transgenic ApoE3Leiden mice with histology, targeted lipidomics and biochemical analyses of metabolic pathways to identify differentially regulated processes and site-specific functions. EWAT was found to exhibit physiological zonation. De novo lipogenesis in fat proximal to epididymis was stably low, whereas de novo lipogenesis distal to epididymis and at other locations was down-regulated in response to high-fat diet. The contents of linoleic acid and alpha-linolenic acid in EWAT were increased compared to other depots. Expression of the androgen receptor (Ar) was higher in EWAT than in MWAT and SWAT. We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and alpha-linolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis.

Show MeSH