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Drosophila melanogaster as a model host for the Burkholderia cepacia complex.

Castonguay-Vanier J, Vial L, Tremblay J, Déziel E - PLoS ONE (2010)

Bottom Line: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis.While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations.Moreover, CI results indicate that this method is more sensitive than mortality tests.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Scientifique-Institut Armand Frappier, Laval, Canada.

ABSTRACT

Background: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis. In order to investigate the virulence of the Bcc, several alternative infection models have been developed. To this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. Moreover, its relevance as a suitable alternative to mammalian hosts has been confirmed with vertebrate organisms.

Methodology/principal findings: The aim of this study was to establish Drosophila melanogaster as a surrogate host for species from the Bcc. While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations. Results obtained with the fruit fly model are comparable with results obtained using mammalian infection models. Furthermore, validity of the Drosophila infection model was confirmed with B. cenocepacia K56-2 mutants known to be less virulent in murine hosts or in other alternative models. Competitive index (CI) analyses were also performed using the fruit fly as host. Results of CI experiments agree with those obtained with mammalian models.

Conclusions/significance: We conclude that Drosophila is a useful alternative infection model for Bcc and that fly pricking assays and competition indices are two complementary methods for virulence testing. Moreover, CI results indicate that this method is more sensitive than mortality tests.

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Relative bacterial load kinetic of fruit flies infected with various Bcc species.At the indicated time points, bacterial load was quantified from living fruit flies as described in Materials and Methods. A: B. cenocepacia LMG18830, B: B. cenocepacia K56-2, C: B. cepacia LMG18821.
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pone-0011467-g004: Relative bacterial load kinetic of fruit flies infected with various Bcc species.At the indicated time points, bacterial load was quantified from living fruit flies as described in Materials and Methods. A: B. cenocepacia LMG18830, B: B. cenocepacia K56-2, C: B. cepacia LMG18821.

Mentions: To verify the presence of bacteria in pricked flies throughout the infections, bacterial survival in vivo was measured for three Bcc strains. Figure 4 demonstrates that every strain tested was capable of colonizing the fly and able to replicate inside the host, although with different rates.


Drosophila melanogaster as a model host for the Burkholderia cepacia complex.

Castonguay-Vanier J, Vial L, Tremblay J, Déziel E - PLoS ONE (2010)

Relative bacterial load kinetic of fruit flies infected with various Bcc species.At the indicated time points, bacterial load was quantified from living fruit flies as described in Materials and Methods. A: B. cenocepacia LMG18830, B: B. cenocepacia K56-2, C: B. cepacia LMG18821.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2902503&req=5

pone-0011467-g004: Relative bacterial load kinetic of fruit flies infected with various Bcc species.At the indicated time points, bacterial load was quantified from living fruit flies as described in Materials and Methods. A: B. cenocepacia LMG18830, B: B. cenocepacia K56-2, C: B. cepacia LMG18821.
Mentions: To verify the presence of bacteria in pricked flies throughout the infections, bacterial survival in vivo was measured for three Bcc strains. Figure 4 demonstrates that every strain tested was capable of colonizing the fly and able to replicate inside the host, although with different rates.

Bottom Line: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis.While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations.Moreover, CI results indicate that this method is more sensitive than mortality tests.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Scientifique-Institut Armand Frappier, Laval, Canada.

ABSTRACT

Background: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis. In order to investigate the virulence of the Bcc, several alternative infection models have been developed. To this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. Moreover, its relevance as a suitable alternative to mammalian hosts has been confirmed with vertebrate organisms.

Methodology/principal findings: The aim of this study was to establish Drosophila melanogaster as a surrogate host for species from the Bcc. While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations. Results obtained with the fruit fly model are comparable with results obtained using mammalian infection models. Furthermore, validity of the Drosophila infection model was confirmed with B. cenocepacia K56-2 mutants known to be less virulent in murine hosts or in other alternative models. Competitive index (CI) analyses were also performed using the fruit fly as host. Results of CI experiments agree with those obtained with mammalian models.

Conclusions/significance: We conclude that Drosophila is a useful alternative infection model for Bcc and that fly pricking assays and competition indices are two complementary methods for virulence testing. Moreover, CI results indicate that this method is more sensitive than mortality tests.

Show MeSH
Related in: MedlinePlus