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Drosophila melanogaster as a model host for the Burkholderia cepacia complex.

Castonguay-Vanier J, Vial L, Tremblay J, Déziel E - PLoS ONE (2010)

Bottom Line: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis.While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations.Moreover, CI results indicate that this method is more sensitive than mortality tests.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Scientifique-Institut Armand Frappier, Laval, Canada.

ABSTRACT

Background: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis. In order to investigate the virulence of the Bcc, several alternative infection models have been developed. To this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. Moreover, its relevance as a suitable alternative to mammalian hosts has been confirmed with vertebrate organisms.

Methodology/principal findings: The aim of this study was to establish Drosophila melanogaster as a surrogate host for species from the Bcc. While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations. Results obtained with the fruit fly model are comparable with results obtained using mammalian infection models. Furthermore, validity of the Drosophila infection model was confirmed with B. cenocepacia K56-2 mutants known to be less virulent in murine hosts or in other alternative models. Competitive index (CI) analyses were also performed using the fruit fly as host. Results of CI experiments agree with those obtained with mammalian models.

Conclusions/significance: We conclude that Drosophila is a useful alternative infection model for Bcc and that fly pricking assays and competition indices are two complementary methods for virulence testing. Moreover, CI results indicate that this method is more sensitive than mortality tests.

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Related in: MedlinePlus

Survival curves for D. melanogaster infected with Bcc strains.Pricking assays were performed with a minimum of 30 flies for each strain. A: B. cepacia LMG1222, B: B. cepacia LMG18821, C: B. multivorans LMG16660, D: B. stabilis LMG18870, E: B. vietnamiensis LMG22486, F: B. vietnamiensis LMG18835, G: B. dolosa LMG21819, H: B. dolosa LMG21443, I: B. ambifaria AU0212, J: B. ambifaria CEP0996, K: B. pyrrocinia LMG21824, L: B. ubonensis LMG20358.
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pone-0011467-g003: Survival curves for D. melanogaster infected with Bcc strains.Pricking assays were performed with a minimum of 30 flies for each strain. A: B. cepacia LMG1222, B: B. cepacia LMG18821, C: B. multivorans LMG16660, D: B. stabilis LMG18870, E: B. vietnamiensis LMG22486, F: B. vietnamiensis LMG18835, G: B. dolosa LMG21819, H: B. dolosa LMG21443, I: B. ambifaria AU0212, J: B. ambifaria CEP0996, K: B. pyrrocinia LMG21824, L: B. ubonensis LMG20358.

Mentions: A number of other Bcc genomovars were then tested and found to display large differences in virulence toward D. melanogaster (Figure 3). B. cepacia strains were from different sources (one was first isolated from onions while the other is a clinical strain) and yet, both are among the most virulent strains in the fly pricking model (Figure 3A and B). They kill 100% of the flies within 3 days. These results are similar to what has been obtained with other hosts, murine or alternative models alike. These data obtained with B. cepacia illustrate also the environmental isolates appear particularly virulent in alternative infection models, and sometimes more virulent than the clinical isolates as also observed with the C. elegans model [19].


Drosophila melanogaster as a model host for the Burkholderia cepacia complex.

Castonguay-Vanier J, Vial L, Tremblay J, Déziel E - PLoS ONE (2010)

Survival curves for D. melanogaster infected with Bcc strains.Pricking assays were performed with a minimum of 30 flies for each strain. A: B. cepacia LMG1222, B: B. cepacia LMG18821, C: B. multivorans LMG16660, D: B. stabilis LMG18870, E: B. vietnamiensis LMG22486, F: B. vietnamiensis LMG18835, G: B. dolosa LMG21819, H: B. dolosa LMG21443, I: B. ambifaria AU0212, J: B. ambifaria CEP0996, K: B. pyrrocinia LMG21824, L: B. ubonensis LMG20358.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2902503&req=5

pone-0011467-g003: Survival curves for D. melanogaster infected with Bcc strains.Pricking assays were performed with a minimum of 30 flies for each strain. A: B. cepacia LMG1222, B: B. cepacia LMG18821, C: B. multivorans LMG16660, D: B. stabilis LMG18870, E: B. vietnamiensis LMG22486, F: B. vietnamiensis LMG18835, G: B. dolosa LMG21819, H: B. dolosa LMG21443, I: B. ambifaria AU0212, J: B. ambifaria CEP0996, K: B. pyrrocinia LMG21824, L: B. ubonensis LMG20358.
Mentions: A number of other Bcc genomovars were then tested and found to display large differences in virulence toward D. melanogaster (Figure 3). B. cepacia strains were from different sources (one was first isolated from onions while the other is a clinical strain) and yet, both are among the most virulent strains in the fly pricking model (Figure 3A and B). They kill 100% of the flies within 3 days. These results are similar to what has been obtained with other hosts, murine or alternative models alike. These data obtained with B. cepacia illustrate also the environmental isolates appear particularly virulent in alternative infection models, and sometimes more virulent than the clinical isolates as also observed with the C. elegans model [19].

Bottom Line: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis.While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations.Moreover, CI results indicate that this method is more sensitive than mortality tests.

View Article: PubMed Central - PubMed

Affiliation: Institut National de la Recherche Scientifique-Institut Armand Frappier, Laval, Canada.

ABSTRACT

Background: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis. In order to investigate the virulence of the Bcc, several alternative infection models have been developed. To this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. Moreover, its relevance as a suitable alternative to mammalian hosts has been confirmed with vertebrate organisms.

Methodology/principal findings: The aim of this study was to establish Drosophila melanogaster as a surrogate host for species from the Bcc. While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations. Results obtained with the fruit fly model are comparable with results obtained using mammalian infection models. Furthermore, validity of the Drosophila infection model was confirmed with B. cenocepacia K56-2 mutants known to be less virulent in murine hosts or in other alternative models. Competitive index (CI) analyses were also performed using the fruit fly as host. Results of CI experiments agree with those obtained with mammalian models.

Conclusions/significance: We conclude that Drosophila is a useful alternative infection model for Bcc and that fly pricking assays and competition indices are two complementary methods for virulence testing. Moreover, CI results indicate that this method is more sensitive than mortality tests.

Show MeSH
Related in: MedlinePlus