Limits...
Analysis of pronuclear zygote configurations in 459 clinical pregnancies obtained with assisted reproductive technique procedures.

Nicoli A, Capodanno F, Moscato L, Rondini I, Villani MT, Tuzio A, La Sala GB - Reprod. Biol. Endocrinol. (2010)

Bottom Line: Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.Related to maternal age we found no significant differences in P1 and in P2 configuration.Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality.

View Article: PubMed Central - HTML - PubMed

Affiliation: Sterility Centre P. Bertocchi, Department of Obstetrics and Gynecology, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. nicoli.alessia@asmn.re.it

ABSTRACT

Background: Embryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability.

Methods: To correlate the zygote-score with the maternal age and the outcome of pregnancy, we analyzed the pronuclear and nucleolar morphology, the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, Italy. We derived odds ratios (OR) and Corenfield's 95% confidence intervals (CI). Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.

Results: We observed a significant increase of "A" pronuclear morphology configuration in 38-41 years old patients in comparison to that lower than or equal to 32 years old and a significant decrease of "B" configuration in 38-41 years old patients in comparison to that lower than or equal to 32 and in comparison to that of 33-37 years old. Related to maternal age we found no significant differences in P1 and in P2 configuration. We found no correlation between zygote-score, embryo cleavage and embryo quality.

Conclusions: Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality. The evaluation of embryo quality based on morphological parameters is probably more predictive than zygote-score.

Show MeSH
P1 and P2 distributions in ongoing pregnancies/deliveries group. Pattern 1 (P1) and Pattern 2 (P2) distributions in ongoing pregnancies/deliveries groups. We classified any zygote as P1 or P2 on the basis of 2PN zygote score: we considered P1 all zygotes classified as A1α, A2β and A3β, and P2 all the other zygotes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2902489&req=5

Figure 3: P1 and P2 distributions in ongoing pregnancies/deliveries group. Pattern 1 (P1) and Pattern 2 (P2) distributions in ongoing pregnancies/deliveries groups. We classified any zygote as P1 or P2 on the basis of 2PN zygote score: we considered P1 all zygotes classified as A1α, A2β and A3β, and P2 all the other zygotes.

Mentions: Moreover, we found no statistically significant differences correlating P1 (A1α, A2β and A3β) and P2 (pool of all the other configurations) configurations with embryo cleavage and embryo quality in clinical pregnancies (Table 5). We observed a similar trend in ongoing pregnancies/deliveries (data not showed). Finally, we found no statistically significant differences in P1 and P2 configurations related to maternal age in clinical pregnancies. Similarly, we found the same result in ongoing pregnancies/deliveries (Figure 3).


Analysis of pronuclear zygote configurations in 459 clinical pregnancies obtained with assisted reproductive technique procedures.

Nicoli A, Capodanno F, Moscato L, Rondini I, Villani MT, Tuzio A, La Sala GB - Reprod. Biol. Endocrinol. (2010)

P1 and P2 distributions in ongoing pregnancies/deliveries group. Pattern 1 (P1) and Pattern 2 (P2) distributions in ongoing pregnancies/deliveries groups. We classified any zygote as P1 or P2 on the basis of 2PN zygote score: we considered P1 all zygotes classified as A1α, A2β and A3β, and P2 all the other zygotes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2902489&req=5

Figure 3: P1 and P2 distributions in ongoing pregnancies/deliveries group. Pattern 1 (P1) and Pattern 2 (P2) distributions in ongoing pregnancies/deliveries groups. We classified any zygote as P1 or P2 on the basis of 2PN zygote score: we considered P1 all zygotes classified as A1α, A2β and A3β, and P2 all the other zygotes.
Mentions: Moreover, we found no statistically significant differences correlating P1 (A1α, A2β and A3β) and P2 (pool of all the other configurations) configurations with embryo cleavage and embryo quality in clinical pregnancies (Table 5). We observed a similar trend in ongoing pregnancies/deliveries (data not showed). Finally, we found no statistically significant differences in P1 and P2 configurations related to maternal age in clinical pregnancies. Similarly, we found the same result in ongoing pregnancies/deliveries (Figure 3).

Bottom Line: Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.Related to maternal age we found no significant differences in P1 and in P2 configuration.Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality.

View Article: PubMed Central - HTML - PubMed

Affiliation: Sterility Centre P. Bertocchi, Department of Obstetrics and Gynecology, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. nicoli.alessia@asmn.re.it

ABSTRACT

Background: Embryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability.

Methods: To correlate the zygote-score with the maternal age and the outcome of pregnancy, we analyzed the pronuclear and nucleolar morphology, the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, Italy. We derived odds ratios (OR) and Corenfield's 95% confidence intervals (CI). Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.

Results: We observed a significant increase of "A" pronuclear morphology configuration in 38-41 years old patients in comparison to that lower than or equal to 32 years old and a significant decrease of "B" configuration in 38-41 years old patients in comparison to that lower than or equal to 32 and in comparison to that of 33-37 years old. Related to maternal age we found no significant differences in P1 and in P2 configuration. We found no correlation between zygote-score, embryo cleavage and embryo quality.

Conclusions: Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality. The evaluation of embryo quality based on morphological parameters is probably more predictive than zygote-score.

Show MeSH