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Analysis of pronuclear zygote configurations in 459 clinical pregnancies obtained with assisted reproductive technique procedures.

Nicoli A, Capodanno F, Moscato L, Rondini I, Villani MT, Tuzio A, La Sala GB - Reprod. Biol. Endocrinol. (2010)

Bottom Line: Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.Related to maternal age we found no significant differences in P1 and in P2 configuration.Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality.

View Article: PubMed Central - HTML - PubMed

Affiliation: Sterility Centre P. Bertocchi, Department of Obstetrics and Gynecology, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. nicoli.alessia@asmn.re.it

ABSTRACT

Background: Embryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability.

Methods: To correlate the zygote-score with the maternal age and the outcome of pregnancy, we analyzed the pronuclear and nucleolar morphology, the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, Italy. We derived odds ratios (OR) and Corenfield's 95% confidence intervals (CI). Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.

Results: We observed a significant increase of "A" pronuclear morphology configuration in 38-41 years old patients in comparison to that lower than or equal to 32 years old and a significant decrease of "B" configuration in 38-41 years old patients in comparison to that lower than or equal to 32 and in comparison to that of 33-37 years old. Related to maternal age we found no significant differences in P1 and in P2 configuration. We found no correlation between zygote-score, embryo cleavage and embryo quality.

Conclusions: Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality. The evaluation of embryo quality based on morphological parameters is probably more predictive than zygote-score.

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Different configurations used for pronuclear morphology, nucleolar morphology and polar body alignement assessment. Pronuclear morphology is classified as A-E, nucleolar morphology is classified as 1-4 and polar body alignement is classified as α, β or γ (9).
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Figure 1: Different configurations used for pronuclear morphology, nucleolar morphology and polar body alignement assessment. Pronuclear morphology is classified as A-E, nucleolar morphology is classified as 1-4 and polar body alignement is classified as α, β or γ (9).

Mentions: For pronuclear morphology score five different configurations regarding pronuclear morphology (A-E), four configurations regarding nucleolar morphology (1, 2, 3, 4) and three configurations regarding polar body alignment (α, β, γ) were adopted as reported by Gianaroli and coworkers [9] (Figure 1).


Analysis of pronuclear zygote configurations in 459 clinical pregnancies obtained with assisted reproductive technique procedures.

Nicoli A, Capodanno F, Moscato L, Rondini I, Villani MT, Tuzio A, La Sala GB - Reprod. Biol. Endocrinol. (2010)

Different configurations used for pronuclear morphology, nucleolar morphology and polar body alignement assessment. Pronuclear morphology is classified as A-E, nucleolar morphology is classified as 1-4 and polar body alignement is classified as α, β or γ (9).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2902489&req=5

Figure 1: Different configurations used for pronuclear morphology, nucleolar morphology and polar body alignement assessment. Pronuclear morphology is classified as A-E, nucleolar morphology is classified as 1-4 and polar body alignement is classified as α, β or γ (9).
Mentions: For pronuclear morphology score five different configurations regarding pronuclear morphology (A-E), four configurations regarding nucleolar morphology (1, 2, 3, 4) and three configurations regarding polar body alignment (α, β, γ) were adopted as reported by Gianaroli and coworkers [9] (Figure 1).

Bottom Line: Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.Related to maternal age we found no significant differences in P1 and in P2 configuration.Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality.

View Article: PubMed Central - HTML - PubMed

Affiliation: Sterility Centre P. Bertocchi, Department of Obstetrics and Gynecology, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. nicoli.alessia@asmn.re.it

ABSTRACT

Background: Embryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability.

Methods: To correlate the zygote-score with the maternal age and the outcome of pregnancy, we analyzed the pronuclear and nucleolar morphology, the polar body alignment and the zygote configuration in 459 clinical pregnancies obtained by IVF and ICSI in our public clinic in Reggio Emilia, Italy. We derived odds ratios (OR) and Corenfield's 95% confidence intervals (CI). Continuous variables were compared with Student's t-test; P lower than .05 was considered statistically significant.

Results: We observed a significant increase of "A" pronuclear morphology configuration in 38-41 years old patients in comparison to that lower than or equal to 32 years old and a significant decrease of "B" configuration in 38-41 years old patients in comparison to that lower than or equal to 32 and in comparison to that of 33-37 years old. Related to maternal age we found no significant differences in P1 and in P2 configuration. We found no correlation between zygote-score, embryo cleavage and embryo quality.

Conclusions: Our results confirm the limited clinical significance of zygote-score suggesting that it can not be associated with maternal age, embryo cleavage and embryo quality. The evaluation of embryo quality based on morphological parameters is probably more predictive than zygote-score.

Show MeSH