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The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex.

Lee M, Choi B, Kwon HJ, Shim JA, Park KS, Lee ES, Sohn S - J Inflamm (Lond) (2010)

Bottom Line: In this study, we evaluated the level of Qa-2, a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G, to determine if it was associated with various symptoms of BD-like mice.The silencing of Qa-2 by intravenous injection of siRNA (500 nmol/mouse, 4 times at 3-day intervals) specifically reduced the Qa-2 levels and worsened the BD-like symptoms.Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Cell Biology, Ajou University Institute for Medical Sciences, Suwon, Korea. sohnsh@ajou.ac.kr.

ABSTRACT

Background: It has been suggested that the HLA-G molecule is a genetic risk factor for Behcet's disease (BD). In this study, we evaluated the level of Qa-2, a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G, to determine if it was associated with various symptoms of BD-like mice. In addition, we investigated siRNA (small interfering RNA) treatment to determine if it inhibited Qa-2 expression, thereby changing the symptoms of mice.

Methods: RNA interference (RNAi) and vector transfection were employed to manipulate gene expression in vivo in mice. siRNA (small interfering RNA) or Qa-2 expression vector was applied to inhibit or up-regulate Qa-2 expression, respectively.

Results: The Qa-2 levels in granulocytes were lower in BD-like mice than in normal controls. The silencing of Qa-2 by intravenous injection of siRNA (500 nmol/mouse, 4 times at 3-day intervals) specifically reduced the Qa-2 levels and worsened the BD-like symptoms.

Conclusions: Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice.

No MeSH data available.


Related in: MedlinePlus

The frequency of NK cells in BD and BDN mice. The frequency of NK cells was analyzed in BD and BDN mice using flow cytometry.
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Figure 9: The frequency of NK cells in BD and BDN mice. The frequency of NK cells was analyzed in BD and BDN mice using flow cytometry.

Mentions: To confirm the relationship between HLA-G and the NK cells, the frequency of NK cells was observed in BD and BDN mice using flow cytometry. As shown in Figure 9, the frequency of NK cells in splenocytes was 13.8 ± 2.2% in BD mice (n = 9) when compared to BDN mice (5.4 ± 0.3%) (n = 5, p < 0.001) and normal mice (8.9 ± 1.1%) (n = 7, p < 0.001). The frequency of NK cells in BD mice was higher than BDN. These findings indicate that down-regulation of HLA-G may influence the higher frequency of NK cells in BD mice.


The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex.

Lee M, Choi B, Kwon HJ, Shim JA, Park KS, Lee ES, Sohn S - J Inflamm (Lond) (2010)

The frequency of NK cells in BD and BDN mice. The frequency of NK cells was analyzed in BD and BDN mice using flow cytometry.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2902457&req=5

Figure 9: The frequency of NK cells in BD and BDN mice. The frequency of NK cells was analyzed in BD and BDN mice using flow cytometry.
Mentions: To confirm the relationship between HLA-G and the NK cells, the frequency of NK cells was observed in BD and BDN mice using flow cytometry. As shown in Figure 9, the frequency of NK cells in splenocytes was 13.8 ± 2.2% in BD mice (n = 9) when compared to BDN mice (5.4 ± 0.3%) (n = 5, p < 0.001) and normal mice (8.9 ± 1.1%) (n = 7, p < 0.001). The frequency of NK cells in BD mice was higher than BDN. These findings indicate that down-regulation of HLA-G may influence the higher frequency of NK cells in BD mice.

Bottom Line: In this study, we evaluated the level of Qa-2, a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G, to determine if it was associated with various symptoms of BD-like mice.The silencing of Qa-2 by intravenous injection of siRNA (500 nmol/mouse, 4 times at 3-day intervals) specifically reduced the Qa-2 levels and worsened the BD-like symptoms.Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Cell Biology, Ajou University Institute for Medical Sciences, Suwon, Korea. sohnsh@ajou.ac.kr.

ABSTRACT

Background: It has been suggested that the HLA-G molecule is a genetic risk factor for Behcet's disease (BD). In this study, we evaluated the level of Qa-2, a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G, to determine if it was associated with various symptoms of BD-like mice. In addition, we investigated siRNA (small interfering RNA) treatment to determine if it inhibited Qa-2 expression, thereby changing the symptoms of mice.

Methods: RNA interference (RNAi) and vector transfection were employed to manipulate gene expression in vivo in mice. siRNA (small interfering RNA) or Qa-2 expression vector was applied to inhibit or up-regulate Qa-2 expression, respectively.

Results: The Qa-2 levels in granulocytes were lower in BD-like mice than in normal controls. The silencing of Qa-2 by intravenous injection of siRNA (500 nmol/mouse, 4 times at 3-day intervals) specifically reduced the Qa-2 levels and worsened the BD-like symptoms.

Conclusions: Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice.

No MeSH data available.


Related in: MedlinePlus