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Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft.

Puricelli E, Corsetti A, Ponzoni D, Martins GL, Leite MG, Santos LA - Head Face Med (2010)

Bottom Line: The histological analyses showed the following results.Moderate interruption on the ostectomized cortical bone.Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone.

View Article: PubMed Central - HTML - PubMed

Affiliation: Oral and Maxillofacial Surgery Unit, Hospital de Clinicas de P.A., School of Dentistry, UFRGS, Porto Alegre, RS, Brazil. epuricelli@uol.com.br

ABSTRACT

Background: In this study, the biocompatibility, stability and osteotransductivity of a new cement based on alpha-tricalcium phosphate (alpha-TCP) were investigated in a bone repair model using a rat model.

Methods: The potential of alpha-TCP on bone repair was compared to autogenous bone grafting, and unfilled cavities were used as negative control. Surgical cavities were prepared and designated as test (T), implanted with alpha-TCP blocks; negative control (C - ), unfilled; and positive control (C + ), implanted with autogenous bone graft. Results were analyzed on postoperative days three, seven, 14, 21 and 60.

Results: The histological analyses showed the following results. Postoperative day three: presence of inflammatory infiltrate, erythrocytes and proliferating fibroblasts in T, C - and C + samples. Day seven: extensive bone neoformation in groups T and C + , and beginning of alpha-TCP resorption by phagocytic cells. Days 14 and 21: osteoblastic activity in the three types of cavities. Day 60: In all samples, neoformed bone similar to surrounding bone. Moderate interruption on the ostectomized cortical bone.

Conclusions: Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone. Comparison of C - with T and C + samples showed that repair is faster in implanted cavities; on day 60, control groups presented almost complete bone repair. Alpha-TCP cement presents biocompatibility and osteotransductivity, besides stability, but 60 days after surgery the cavities were not closed.

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Histological aspects of samples collected on postoperative day 14. A, T cavity, Alpha-tricalcium phosphate (alpha-TCP) block implanted in the bone cavity. The material shows irregularities on its surface and interior, evidencing bone neoformation. (Magnification 40×). B, C - cavity, OR In the graft-free cavity, leveling of trabecular bone prevents the closure of the bone wound. (Magnification 40×). C, C + cavity, The grafted segments drift apart, but keep parallel orientation. Extensive fibrosis is observed between the bone grafts. (Magnification 40×).
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Figure 6: Histological aspects of samples collected on postoperative day 14. A, T cavity, Alpha-tricalcium phosphate (alpha-TCP) block implanted in the bone cavity. The material shows irregularities on its surface and interior, evidencing bone neoformation. (Magnification 40×). B, C - cavity, OR In the graft-free cavity, leveling of trabecular bone prevents the closure of the bone wound. (Magnification 40×). C, C + cavity, The grafted segments drift apart, but keep parallel orientation. Extensive fibrosis is observed between the bone grafts. (Magnification 40×).

Mentions: Representative results observed on the different postoperative days are presented in Figures 3, 4, 5, 6, 7 and 8 (days 3, 7, 14, 21 and 60, respectively). Within each group, histological characteristics were described for each of the surgical cavities. To evaluate the process of bone healing, sections were analyzed for the amount of neoformed bone, presence of an inflammatory infiltrate, and reaction against a foreign body.


Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft.

Puricelli E, Corsetti A, Ponzoni D, Martins GL, Leite MG, Santos LA - Head Face Med (2010)

Histological aspects of samples collected on postoperative day 14. A, T cavity, Alpha-tricalcium phosphate (alpha-TCP) block implanted in the bone cavity. The material shows irregularities on its surface and interior, evidencing bone neoformation. (Magnification 40×). B, C - cavity, OR In the graft-free cavity, leveling of trabecular bone prevents the closure of the bone wound. (Magnification 40×). C, C + cavity, The grafted segments drift apart, but keep parallel orientation. Extensive fibrosis is observed between the bone grafts. (Magnification 40×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2902431&req=5

Figure 6: Histological aspects of samples collected on postoperative day 14. A, T cavity, Alpha-tricalcium phosphate (alpha-TCP) block implanted in the bone cavity. The material shows irregularities on its surface and interior, evidencing bone neoformation. (Magnification 40×). B, C - cavity, OR In the graft-free cavity, leveling of trabecular bone prevents the closure of the bone wound. (Magnification 40×). C, C + cavity, The grafted segments drift apart, but keep parallel orientation. Extensive fibrosis is observed between the bone grafts. (Magnification 40×).
Mentions: Representative results observed on the different postoperative days are presented in Figures 3, 4, 5, 6, 7 and 8 (days 3, 7, 14, 21 and 60, respectively). Within each group, histological characteristics were described for each of the surgical cavities. To evaluate the process of bone healing, sections were analyzed for the amount of neoformed bone, presence of an inflammatory infiltrate, and reaction against a foreign body.

Bottom Line: The histological analyses showed the following results.Moderate interruption on the ostectomized cortical bone.Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone.

View Article: PubMed Central - HTML - PubMed

Affiliation: Oral and Maxillofacial Surgery Unit, Hospital de Clinicas de P.A., School of Dentistry, UFRGS, Porto Alegre, RS, Brazil. epuricelli@uol.com.br

ABSTRACT

Background: In this study, the biocompatibility, stability and osteotransductivity of a new cement based on alpha-tricalcium phosphate (alpha-TCP) were investigated in a bone repair model using a rat model.

Methods: The potential of alpha-TCP on bone repair was compared to autogenous bone grafting, and unfilled cavities were used as negative control. Surgical cavities were prepared and designated as test (T), implanted with alpha-TCP blocks; negative control (C - ), unfilled; and positive control (C + ), implanted with autogenous bone graft. Results were analyzed on postoperative days three, seven, 14, 21 and 60.

Results: The histological analyses showed the following results. Postoperative day three: presence of inflammatory infiltrate, erythrocytes and proliferating fibroblasts in T, C - and C + samples. Day seven: extensive bone neoformation in groups T and C + , and beginning of alpha-TCP resorption by phagocytic cells. Days 14 and 21: osteoblastic activity in the three types of cavities. Day 60: In all samples, neoformed bone similar to surrounding bone. Moderate interruption on the ostectomized cortical bone.

Conclusions: Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone. Comparison of C - with T and C + samples showed that repair is faster in implanted cavities; on day 60, control groups presented almost complete bone repair. Alpha-TCP cement presents biocompatibility and osteotransductivity, besides stability, but 60 days after surgery the cavities were not closed.

Show MeSH
Related in: MedlinePlus