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Further increase in the expression of activation markers on monocyte-derived dendritic cells in coronary artery disease patients with ectasia compared to patients with coronary artery disease alone.

Yildirim N, Tekin IO, Arasli M, Aydin M - Mediators Inflamm. (2010)

Bottom Line: CAD patients with CAE were detected to have significantly higher mean fluorescence intensities of CD11b, CD11c, CD54 , CD83, CD86 and MHC Class II molecules on mDCs in comparison to CAD patients without CAE and normal controls (P < .001 for all).A significant positive correlation was found between the number of vessels with CAE and the levels of CD11c, CD86, and MHC Class II molecules. mDCs display an increased cell surface concentration of activation molecules in CAD patients with CAE compared to patients with CAD alone.DC activation may play an important role for CAE development in patients with CAD.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey. nesligul2004@hotmail.com

ABSTRACT

Background: Coronary artery ectasia (CAE) is defined as localized or diffuse dilation of the coronary arteries. There are scarce data about the role of dendritic cells in CAE development. In this study we investigated the activation markers on the surface of monocyte-derived dendritic cells (mDCs) in coronary artery disease (CAD) patients with or without CAE.

Method: The study consisted of 6 patients who had obstructive CAD with CAE, 6 CAD patients without CAE and 6 subjects with angiographically normal coronary arteries. mDCs were cultivated from peripheral blood monocytes. Surface activation markers were detected by flow cytometry.

Results: CAD patients with CAE were detected to have significantly higher mean fluorescence intensities of CD11b, CD11c, CD54 , CD83, CD86 and MHC Class II molecules on mDCs in comparison to CAD patients without CAE and normal controls (P < .001 for all). A significant positive correlation was found between the number of vessels with CAE and the levels of CD11c, CD86, and MHC Class II molecules.

Conclusion: mDCs display an increased cell surface concentration of activation molecules in CAD patients with CAE compared to patients with CAD alone. DC activation may play an important role for CAE development in patients with CAD.

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Related in: MedlinePlus

The graph showing the relationship between the number of ectatic vessels and the expression level of CD86 molecule. r: Spearman's rho correlation coefficient. MFI: mean fluorescence intensity. MFI: mean fluorescence intensity.
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fig3: The graph showing the relationship between the number of ectatic vessels and the expression level of CD86 molecule. r: Spearman's rho correlation coefficient. MFI: mean fluorescence intensity. MFI: mean fluorescence intensity.

Mentions: CAD patients with CAE were detected to have significantly higher levels of certain activation markers such as CD11b (44.5 ± 5.0 versus 30.0 ± 3.8 and 20.9 ± 3.6), CD11c, (96.3 ± 10.9 versus 66.1 ± 6.4 and 50.4 ± 5.7) CD54 (45.6 ± 6.7 versus 31.1 ± 4.9 and 20.8 ± 3.2), CD83 (44.6 ± 6.1 versus 30.8 ± 2.4 and 25.6 ± 2.8), CD86 (50.7 ± 5.0 versus 39.2 ± 4.1 and 29.5 ± 4.1) and MHC Class II (112.4 ± 11.3 versus 73.1 ± 9.5 and 54.5 ± 4.5) molecules on the surface of mDCs in comparison to CAD patients without CAE and normal subjects with angiographically normal coronary arteries (Figure 1). MFI of CD14 on mDCs did not significantly differ among Group 1 (13.3 ± 3.1), Group 2 (12.9 ± 2.6), and Group 3 (14 ± 2.9) (P > .05). Furthermore we detected a significant positive correlation between the number of the vessels with CAE and the levels of CD11c (Figure 2), CD86 (Figure 3), and MHC Class II molecules (Figure 4).


Further increase in the expression of activation markers on monocyte-derived dendritic cells in coronary artery disease patients with ectasia compared to patients with coronary artery disease alone.

Yildirim N, Tekin IO, Arasli M, Aydin M - Mediators Inflamm. (2010)

The graph showing the relationship between the number of ectatic vessels and the expression level of CD86 molecule. r: Spearman's rho correlation coefficient. MFI: mean fluorescence intensity. MFI: mean fluorescence intensity.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2902079&req=5

fig3: The graph showing the relationship between the number of ectatic vessels and the expression level of CD86 molecule. r: Spearman's rho correlation coefficient. MFI: mean fluorescence intensity. MFI: mean fluorescence intensity.
Mentions: CAD patients with CAE were detected to have significantly higher levels of certain activation markers such as CD11b (44.5 ± 5.0 versus 30.0 ± 3.8 and 20.9 ± 3.6), CD11c, (96.3 ± 10.9 versus 66.1 ± 6.4 and 50.4 ± 5.7) CD54 (45.6 ± 6.7 versus 31.1 ± 4.9 and 20.8 ± 3.2), CD83 (44.6 ± 6.1 versus 30.8 ± 2.4 and 25.6 ± 2.8), CD86 (50.7 ± 5.0 versus 39.2 ± 4.1 and 29.5 ± 4.1) and MHC Class II (112.4 ± 11.3 versus 73.1 ± 9.5 and 54.5 ± 4.5) molecules on the surface of mDCs in comparison to CAD patients without CAE and normal subjects with angiographically normal coronary arteries (Figure 1). MFI of CD14 on mDCs did not significantly differ among Group 1 (13.3 ± 3.1), Group 2 (12.9 ± 2.6), and Group 3 (14 ± 2.9) (P > .05). Furthermore we detected a significant positive correlation between the number of the vessels with CAE and the levels of CD11c (Figure 2), CD86 (Figure 3), and MHC Class II molecules (Figure 4).

Bottom Line: CAD patients with CAE were detected to have significantly higher mean fluorescence intensities of CD11b, CD11c, CD54 , CD83, CD86 and MHC Class II molecules on mDCs in comparison to CAD patients without CAE and normal controls (P < .001 for all).A significant positive correlation was found between the number of vessels with CAE and the levels of CD11c, CD86, and MHC Class II molecules. mDCs display an increased cell surface concentration of activation molecules in CAD patients with CAE compared to patients with CAD alone.DC activation may play an important role for CAE development in patients with CAD.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey. nesligul2004@hotmail.com

ABSTRACT

Background: Coronary artery ectasia (CAE) is defined as localized or diffuse dilation of the coronary arteries. There are scarce data about the role of dendritic cells in CAE development. In this study we investigated the activation markers on the surface of monocyte-derived dendritic cells (mDCs) in coronary artery disease (CAD) patients with or without CAE.

Method: The study consisted of 6 patients who had obstructive CAD with CAE, 6 CAD patients without CAE and 6 subjects with angiographically normal coronary arteries. mDCs were cultivated from peripheral blood monocytes. Surface activation markers were detected by flow cytometry.

Results: CAD patients with CAE were detected to have significantly higher mean fluorescence intensities of CD11b, CD11c, CD54 , CD83, CD86 and MHC Class II molecules on mDCs in comparison to CAD patients without CAE and normal controls (P < .001 for all). A significant positive correlation was found between the number of vessels with CAE and the levels of CD11c, CD86, and MHC Class II molecules.

Conclusion: mDCs display an increased cell surface concentration of activation molecules in CAD patients with CAE compared to patients with CAD alone. DC activation may play an important role for CAE development in patients with CAD.

Show MeSH
Related in: MedlinePlus