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New automated and high-throughput quantitative analysis of urinary ketones by multifiber exchange-solid phase microextraction coupled to fast gas chromatography/negative chemical-electron ionization/mass spectrometry.

Pacenti M, Dugheri S, Traldi P, Degli Esposti F, Perchiazzi N, Franchi E, Calamante M, Kikic I, Alessi P, Bonacchi A, Salvadori E, Arcangeli G, Cupelli V - J Autom Methods Manag Chem (2010)

Bottom Line: The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones.The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01-5.32%).The detection limits for the seven ketones were less than 0.004 mg/L.

View Article: PubMed Central - PubMed

Affiliation: Occupational Health Division, Department of Public Health, Viale Morgagni 48, University of Florence, 50100 Florence, Italy.

ABSTRACT
The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01-5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.

No MeSH data available.


Related in: MedlinePlus

XYZ autosampler equipped with 3-position trays MultiFiber Exchange and SPME Fast Fit Assembled.
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fig1: XYZ autosampler equipped with 3-position trays MultiFiber Exchange and SPME Fast Fit Assembled.

Mentions: New fully automation of the procedure was achieved using a Gerstel MPS2 autosampler equipped with 3-position trays MFX (SRA Instruments, Milan, Italy) and Fast Fit Assemblies (FFAs)-SPME fibers (Supelco, Sigma-Aldrich, Milan, Italy) patented by Chromline (Prato, Italy). FFA is the device that allows to use fibers for fully automated processes. It is formed by adaptors that make the fiber much more robust and identifiable by its barcode. Thanks to FFA it is possible to change SPME fibers in automatic mode by MFX (Figure 1). In this option the fibers are transported between the 3-position tray, the vials, containing urine or derivatization agents, and the injector by new SPME holder equipped with a plunger/magnetic system. At the end of the analysis desorbed fiber is moved back to the tray and the cycle is repeated. Moreover, the autosampler parameters allowed to complete the analysis cycle by conditioning and sealing in the Teflon septum inserted into the tray. The automatically change of SPME fibers and processing using XYZ autosampler is indicated as flowchart in Figure 2.


New automated and high-throughput quantitative analysis of urinary ketones by multifiber exchange-solid phase microextraction coupled to fast gas chromatography/negative chemical-electron ionization/mass spectrometry.

Pacenti M, Dugheri S, Traldi P, Degli Esposti F, Perchiazzi N, Franchi E, Calamante M, Kikic I, Alessi P, Bonacchi A, Salvadori E, Arcangeli G, Cupelli V - J Autom Methods Manag Chem (2010)

XYZ autosampler equipped with 3-position trays MultiFiber Exchange and SPME Fast Fit Assembled.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2902044&req=5

fig1: XYZ autosampler equipped with 3-position trays MultiFiber Exchange and SPME Fast Fit Assembled.
Mentions: New fully automation of the procedure was achieved using a Gerstel MPS2 autosampler equipped with 3-position trays MFX (SRA Instruments, Milan, Italy) and Fast Fit Assemblies (FFAs)-SPME fibers (Supelco, Sigma-Aldrich, Milan, Italy) patented by Chromline (Prato, Italy). FFA is the device that allows to use fibers for fully automated processes. It is formed by adaptors that make the fiber much more robust and identifiable by its barcode. Thanks to FFA it is possible to change SPME fibers in automatic mode by MFX (Figure 1). In this option the fibers are transported between the 3-position tray, the vials, containing urine or derivatization agents, and the injector by new SPME holder equipped with a plunger/magnetic system. At the end of the analysis desorbed fiber is moved back to the tray and the cycle is repeated. Moreover, the autosampler parameters allowed to complete the analysis cycle by conditioning and sealing in the Teflon septum inserted into the tray. The automatically change of SPME fibers and processing using XYZ autosampler is indicated as flowchart in Figure 2.

Bottom Line: The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones.The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01-5.32%).The detection limits for the seven ketones were less than 0.004 mg/L.

View Article: PubMed Central - PubMed

Affiliation: Occupational Health Division, Department of Public Health, Viale Morgagni 48, University of Florence, 50100 Florence, Italy.

ABSTRACT
The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01-5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.

No MeSH data available.


Related in: MedlinePlus