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Anti-inflammatory activity of chitooligosaccharides in vivo.

Fernandes JC, Spindola H, de Sousa V, Santos-Silva A, Pintado ME, Malcata FX, Carvalho JE - Mar Drugs (2010)

Bottom Line: This is a widely accepted animal model of acute inflammation to evaluate the anti-inflammatory effect of drugs.Our data suggest that COS possess anti-inflammatory activity, which is dependent on dose and, at higher doses, also on the molecular weight.A single dose of 500 mg/kg b.w. weight may be suitable to treat acute inflammation cases; however, further studies are needed to ascertain the effect upon longer inflammation periods as well as studies upon the bioavailability of these compounds.

View Article: PubMed Central - PubMed

Affiliation: CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, Porto, Portugal. jfernandes@email.com

ABSTRACT
All the reports to date on the anti-inflammatory activity of chitooligosaccharides (COS) are mostly based on in vitro methods. In this work, the anti-inflammatory activity of two COS mixtures is characterized in vivo (using balb/c mice), following the carrageenan-induced paw edema method. This is a widely accepted animal model of acute inflammation to evaluate the anti-inflammatory effect of drugs. Our data suggest that COS possess anti-inflammatory activity, which is dependent on dose and, at higher doses, also on the molecular weight. A single dose of 500 mg/kg b.w. weight may be suitable to treat acute inflammation cases; however, further studies are needed to ascertain the effect upon longer inflammation periods as well as studies upon the bioavailability of these compounds.

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Related in: MedlinePlus

Effect of 500 mg/kg b.w. of both COS, administered orally 60 min prior to injection of carrageenan, on mice paw edema volume (mL), along the time (Average ± S.E.M.).
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f2-marinedrugs-08-01763: Effect of 500 mg/kg b.w. of both COS, administered orally 60 min prior to injection of carrageenan, on mice paw edema volume (mL), along the time (Average ± S.E.M.).

Mentions: Overall every dose of COS3 and COS5 tested in this study showed significant reduction of paw edema at 2 h after carrageenan injection, suggesting that COS produces an anti-edematous effect during the second phase, similarly to INN. Therefore, our results suggest that the mechanism of the anti-inflammatory effect of COS may involve the inhibition of the cyclooxygenase pathway; as reported elsewhere [6], COS may exert their anti-inflammatory effect via down-regulation of transcriptional and translational expression levels of COX-2. At this stage, an endpoint was established in order to prevent animals from suffering a severe discomfort resulting from leg ulceration. However, the experiment was extended with the INN group (which showed to be more effective than Dexa as a positive control), and with the most promising COS concentration–500 mg/kg (Figure 2).


Anti-inflammatory activity of chitooligosaccharides in vivo.

Fernandes JC, Spindola H, de Sousa V, Santos-Silva A, Pintado ME, Malcata FX, Carvalho JE - Mar Drugs (2010)

Effect of 500 mg/kg b.w. of both COS, administered orally 60 min prior to injection of carrageenan, on mice paw edema volume (mL), along the time (Average ± S.E.M.).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2901823&req=5

f2-marinedrugs-08-01763: Effect of 500 mg/kg b.w. of both COS, administered orally 60 min prior to injection of carrageenan, on mice paw edema volume (mL), along the time (Average ± S.E.M.).
Mentions: Overall every dose of COS3 and COS5 tested in this study showed significant reduction of paw edema at 2 h after carrageenan injection, suggesting that COS produces an anti-edematous effect during the second phase, similarly to INN. Therefore, our results suggest that the mechanism of the anti-inflammatory effect of COS may involve the inhibition of the cyclooxygenase pathway; as reported elsewhere [6], COS may exert their anti-inflammatory effect via down-regulation of transcriptional and translational expression levels of COX-2. At this stage, an endpoint was established in order to prevent animals from suffering a severe discomfort resulting from leg ulceration. However, the experiment was extended with the INN group (which showed to be more effective than Dexa as a positive control), and with the most promising COS concentration–500 mg/kg (Figure 2).

Bottom Line: This is a widely accepted animal model of acute inflammation to evaluate the anti-inflammatory effect of drugs.Our data suggest that COS possess anti-inflammatory activity, which is dependent on dose and, at higher doses, also on the molecular weight.A single dose of 500 mg/kg b.w. weight may be suitable to treat acute inflammation cases; however, further studies are needed to ascertain the effect upon longer inflammation periods as well as studies upon the bioavailability of these compounds.

View Article: PubMed Central - PubMed

Affiliation: CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, Porto, Portugal. jfernandes@email.com

ABSTRACT
All the reports to date on the anti-inflammatory activity of chitooligosaccharides (COS) are mostly based on in vitro methods. In this work, the anti-inflammatory activity of two COS mixtures is characterized in vivo (using balb/c mice), following the carrageenan-induced paw edema method. This is a widely accepted animal model of acute inflammation to evaluate the anti-inflammatory effect of drugs. Our data suggest that COS possess anti-inflammatory activity, which is dependent on dose and, at higher doses, also on the molecular weight. A single dose of 500 mg/kg b.w. weight may be suitable to treat acute inflammation cases; however, further studies are needed to ascertain the effect upon longer inflammation periods as well as studies upon the bioavailability of these compounds.

Show MeSH
Related in: MedlinePlus