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In situ aquaculture methods for Dysidea avara (Demospongiae, Porifera) in the northwestern Mediterranean.

de Caralt S, Sánchez-Fontenla J, Uriz MJ, Wijffels RH - Mar Drugs (2010)

Bottom Line: In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes.Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates.While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

View Article: PubMed Central - PubMed

Affiliation: Centre d'Estudis Avançats de Blanes (CEAB-CSIC), Accés a la Cala St Francesc 14, Blanes, Spain. sonia@ceab.csic.es

ABSTRACT
Marine sponges produce secondary metabolites that can be used as a natural source for the design of new drugs and cosmetics. There is, however, a supply problem with these natural substances for research and eventual commercialisation of the products. In situ sponge aquaculture is nowadays one of the most reliable methods to supply pharmaceutical companies with sufficient quantities of the target compound. In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes. The soft consistency of this species makes the traditional culture method based on holding explants on ropes unsuitable. We have tested alternative culture methods for D. avara and optimized the underwater structures to hold the sponges to be used in aquaculture. Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates. Culture efficiency was evaluated by determination of sponge survival, growth rates, and bioactivity (as an indication of production of the target metabolite). While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

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Picture of a D. avara explant growing out of the cage after eight months of culture. The scale bar is 1 cm.
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f4-marinedrugs-08-01731: Picture of a D. avara explant growing out of the cage after eight months of culture. The scale bar is 1 cm.

Mentions: After six months of culture, there were no significant differences among the three culture methods (p > 0.05, Kruskal-Wallis ANOVA test); however the explants cultured on ropes presented the lowest mean growth rate (ca. 40%), while the explants cultured in cages or glued to the frame presented similarly high growth rates (166.75 ± 34.62% and 167.23 ± 42.7; mean ± standard error, respectively). The explants placed in cages attached to the plastic net quite fast and after some weeks some of them grew out of the cage (Figure 4).


In situ aquaculture methods for Dysidea avara (Demospongiae, Porifera) in the northwestern Mediterranean.

de Caralt S, Sánchez-Fontenla J, Uriz MJ, Wijffels RH - Mar Drugs (2010)

Picture of a D. avara explant growing out of the cage after eight months of culture. The scale bar is 1 cm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2901820&req=5

f4-marinedrugs-08-01731: Picture of a D. avara explant growing out of the cage after eight months of culture. The scale bar is 1 cm.
Mentions: After six months of culture, there were no significant differences among the three culture methods (p > 0.05, Kruskal-Wallis ANOVA test); however the explants cultured on ropes presented the lowest mean growth rate (ca. 40%), while the explants cultured in cages or glued to the frame presented similarly high growth rates (166.75 ± 34.62% and 167.23 ± 42.7; mean ± standard error, respectively). The explants placed in cages attached to the plastic net quite fast and after some weeks some of them grew out of the cage (Figure 4).

Bottom Line: In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes.Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates.While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

View Article: PubMed Central - PubMed

Affiliation: Centre d'Estudis Avançats de Blanes (CEAB-CSIC), Accés a la Cala St Francesc 14, Blanes, Spain. sonia@ceab.csic.es

ABSTRACT
Marine sponges produce secondary metabolites that can be used as a natural source for the design of new drugs and cosmetics. There is, however, a supply problem with these natural substances for research and eventual commercialisation of the products. In situ sponge aquaculture is nowadays one of the most reliable methods to supply pharmaceutical companies with sufficient quantities of the target compound. In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes. The soft consistency of this species makes the traditional culture method based on holding explants on ropes unsuitable. We have tested alternative culture methods for D. avara and optimized the underwater structures to hold the sponges to be used in aquaculture. Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates. Culture efficiency was evaluated by determination of sponge survival, growth rates, and bioactivity (as an indication of production of the target metabolite). While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

Show MeSH