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In situ aquaculture methods for Dysidea avara (Demospongiae, Porifera) in the northwestern Mediterranean.

de Caralt S, Sánchez-Fontenla J, Uriz MJ, Wijffels RH - Mar Drugs (2010)

Bottom Line: In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes.Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates.While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

View Article: PubMed Central - PubMed

Affiliation: Centre d'Estudis Avançats de Blanes (CEAB-CSIC), Accés a la Cala St Francesc 14, Blanes, Spain. sonia@ceab.csic.es

ABSTRACT
Marine sponges produce secondary metabolites that can be used as a natural source for the design of new drugs and cosmetics. There is, however, a supply problem with these natural substances for research and eventual commercialisation of the products. In situ sponge aquaculture is nowadays one of the most reliable methods to supply pharmaceutical companies with sufficient quantities of the target compound. In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes. The soft consistency of this species makes the traditional culture method based on holding explants on ropes unsuitable. We have tested alternative culture methods for D. avara and optimized the underwater structures to hold the sponges to be used in aquaculture. Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates. Culture efficiency was evaluated by determination of sponge survival, growth rates, and bioactivity (as an indication of production of the target metabolite). While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

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Underwater picture of the three culture methods assayed: (A) D. avara explant cultured by the rope method (B) cage containing a D. avara explant (C) D. avara explant glued to the metallic frame with the two tie raps holding it. The scale bar is 1 cm.
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f1-marinedrugs-08-01731: Underwater picture of the three culture methods assayed: (A) D. avara explant cultured by the rope method (B) cage containing a D. avara explant (C) D. avara explant glued to the metallic frame with the two tie raps holding it. The scale bar is 1 cm.

Mentions: The rope method used in this study is similar to that already used by other authors [27,33], where a rope was inserted in a large needle and carefully passed through the sponge tissue. The ropes with the explants where placed horizontally, anchored to the rigid frames (Figure 1A). In the second method we placed each explant inside a 6 × 6 × 5 cm cage. The cages were made of a rigid 1 cm mesh size, plastic net to ensure seawater flow through the cages (Figure 1B). The third method consisted of gluing the explants to a horizontal steel frame with a non-toxic, two components resin (IVEGOR) (Figure 1C). Cages, frames and ropes were all placed at a distance of 40 cm from the sea bottom.


In situ aquaculture methods for Dysidea avara (Demospongiae, Porifera) in the northwestern Mediterranean.

de Caralt S, Sánchez-Fontenla J, Uriz MJ, Wijffels RH - Mar Drugs (2010)

Underwater picture of the three culture methods assayed: (A) D. avara explant cultured by the rope method (B) cage containing a D. avara explant (C) D. avara explant glued to the metallic frame with the two tie raps holding it. The scale bar is 1 cm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2901820&req=5

f1-marinedrugs-08-01731: Underwater picture of the three culture methods assayed: (A) D. avara explant cultured by the rope method (B) cage containing a D. avara explant (C) D. avara explant glued to the metallic frame with the two tie raps holding it. The scale bar is 1 cm.
Mentions: The rope method used in this study is similar to that already used by other authors [27,33], where a rope was inserted in a large needle and carefully passed through the sponge tissue. The ropes with the explants where placed horizontally, anchored to the rigid frames (Figure 1A). In the second method we placed each explant inside a 6 × 6 × 5 cm cage. The cages were made of a rigid 1 cm mesh size, plastic net to ensure seawater flow through the cages (Figure 1B). The third method consisted of gluing the explants to a horizontal steel frame with a non-toxic, two components resin (IVEGOR) (Figure 1C). Cages, frames and ropes were all placed at a distance of 40 cm from the sea bottom.

Bottom Line: In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes.Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates.While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

View Article: PubMed Central - PubMed

Affiliation: Centre d'Estudis Avançats de Blanes (CEAB-CSIC), Accés a la Cala St Francesc 14, Blanes, Spain. sonia@ceab.csic.es

ABSTRACT
Marine sponges produce secondary metabolites that can be used as a natural source for the design of new drugs and cosmetics. There is, however, a supply problem with these natural substances for research and eventual commercialisation of the products. In situ sponge aquaculture is nowadays one of the most reliable methods to supply pharmaceutical companies with sufficient quantities of the target compound. In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes. The soft consistency of this species makes the traditional culture method based on holding explants on ropes unsuitable. We have tested alternative culture methods for D. avara and optimized the underwater structures to hold the sponges to be used in aquaculture. Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates. Culture efficiency was evaluated by determination of sponge survival, growth rates, and bioactivity (as an indication of production of the target metabolite). While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

Show MeSH