Limits...
Effect of dietary fats on oxidative-antioxidative status of blood in rats.

Walczewska A, Dziedzic B, Stepien T, Swiatek E, Nowak D - J Clin Biochem Nutr (2010)

Bottom Line: Whole blood chemiluminescence (CL) did not differ between three low-fat fed groups.The highest level of oxidize glutathione (GSSH), the lowest reduce glutathione (GSH)/GSSG ratio in erythrocytes, and the highest plasma activity to reduce ferric ions were observed in rats fed both diets contaning linoleic acid-rich sunflower oil compared to animals fed the corresponding energy from other fats. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of plasma was lower in high-lard and high-FO fed rats compared to the corresponding low-fat diets, and the lowest in low-FO fed rats among low-fat fed animals.We presume from our results that linoleic acid may have dual effect, prooxidative in blood cells but maintaining total antioxidant plasma ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz 92-215, Poland.

ABSTRACT
This study was performed to examine the effect of different fat sources, lard, sunflower oil (SO), and fish oil (FO) in high-fat and low-fat diet on reactive oxygen species generation by blood phagocytes, glutathione redox status in erythrocytes, and total plasma antioxidant ability in rats. Whole blood chemiluminescence (CL) did not differ between three low-fat fed groups. However, baseline and phorbol myristate acetate (PMA)-stimulated CL in blood of high-lard fed rats were lower than in low-lard and high-SO fed animals. Phagocyte-stimulated oxidative burst was higher in rats fed high-SO diet than in those fed low-SO and high-FO diets. The highest level of oxidize glutathione (GSSH), the lowest reduce glutathione (GSH)/GSSG ratio in erythrocytes, and the highest plasma activity to reduce ferric ions were observed in rats fed both diets contaning linoleic acid-rich sunflower oil compared to animals fed the corresponding energy from other fats. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of plasma was lower in high-lard and high-FO fed rats compared to the corresponding low-fat diets, and the lowest in low-FO fed rats among low-fat fed animals. We presume from our results that linoleic acid may have dual effect, prooxidative in blood cells but maintaining total antioxidant plasma ability.

No MeSH data available.


The effect of the low-fat (LF) and high-fat (HF) diets containing lard, sunflower oil, or fish oil on the concentrations of (A) glutathione (GSH), (B) oxidized glutathione (GSSG), and (C) GSH/GSSG ratio in rat erythrocytes. Values are expressed as mean ± SEM (n = 5–8). Significant differences are as follows: a p<0.01 vs other LF or HF diets; b p<0.01 vs low-lard diet; c p<0.01 vs high-lard diet.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2901759&req=5

Figure 2: The effect of the low-fat (LF) and high-fat (HF) diets containing lard, sunflower oil, or fish oil on the concentrations of (A) glutathione (GSH), (B) oxidized glutathione (GSSG), and (C) GSH/GSSG ratio in rat erythrocytes. Values are expressed as mean ± SEM (n = 5–8). Significant differences are as follows: a p<0.01 vs other LF or HF diets; b p<0.01 vs low-lard diet; c p<0.01 vs high-lard diet.

Mentions: There were no significant differences in the erythrocyte GSH and GSSH levels between rats fed low-fat and high-fat diet prepared with the same fats, however there were significant differences in their concentrations between animals fed various fats in the same energy quantity in the diet (Fig. 2). The lowest GSH and the highest GSSH concentrations were observed in RBC of rats fed both low and high SO diets (p<0.001 vs low and high FO; p<0.01 vs low and high lard). Consequently, the GSH/GSSG ratio was the lowest in the erythrocytes of animals fed low and high SO (Fig. 2C). Although the reduced GSH level in the erythrocytes of low-FO fed rats was significantly higher than of low-lard fed animals (p<0.01; Fig. 2A), but the antioxidant status measured as the GSH/GSSG ratio was significantly lower in these rats compared to low- lard fed animals (p<0.01; Fig. 2C). The GSH/GSSG ratio was significantly higher in low and high-lard fed groups compared to animals fed the same energy with SO and FO in the diet (Fig. 2C).


Effect of dietary fats on oxidative-antioxidative status of blood in rats.

Walczewska A, Dziedzic B, Stepien T, Swiatek E, Nowak D - J Clin Biochem Nutr (2010)

The effect of the low-fat (LF) and high-fat (HF) diets containing lard, sunflower oil, or fish oil on the concentrations of (A) glutathione (GSH), (B) oxidized glutathione (GSSG), and (C) GSH/GSSG ratio in rat erythrocytes. Values are expressed as mean ± SEM (n = 5–8). Significant differences are as follows: a p<0.01 vs other LF or HF diets; b p<0.01 vs low-lard diet; c p<0.01 vs high-lard diet.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2901759&req=5

Figure 2: The effect of the low-fat (LF) and high-fat (HF) diets containing lard, sunflower oil, or fish oil on the concentrations of (A) glutathione (GSH), (B) oxidized glutathione (GSSG), and (C) GSH/GSSG ratio in rat erythrocytes. Values are expressed as mean ± SEM (n = 5–8). Significant differences are as follows: a p<0.01 vs other LF or HF diets; b p<0.01 vs low-lard diet; c p<0.01 vs high-lard diet.
Mentions: There were no significant differences in the erythrocyte GSH and GSSH levels between rats fed low-fat and high-fat diet prepared with the same fats, however there were significant differences in their concentrations between animals fed various fats in the same energy quantity in the diet (Fig. 2). The lowest GSH and the highest GSSH concentrations were observed in RBC of rats fed both low and high SO diets (p<0.001 vs low and high FO; p<0.01 vs low and high lard). Consequently, the GSH/GSSG ratio was the lowest in the erythrocytes of animals fed low and high SO (Fig. 2C). Although the reduced GSH level in the erythrocytes of low-FO fed rats was significantly higher than of low-lard fed animals (p<0.01; Fig. 2A), but the antioxidant status measured as the GSH/GSSG ratio was significantly lower in these rats compared to low- lard fed animals (p<0.01; Fig. 2C). The GSH/GSSG ratio was significantly higher in low and high-lard fed groups compared to animals fed the same energy with SO and FO in the diet (Fig. 2C).

Bottom Line: Whole blood chemiluminescence (CL) did not differ between three low-fat fed groups.The highest level of oxidize glutathione (GSSH), the lowest reduce glutathione (GSH)/GSSG ratio in erythrocytes, and the highest plasma activity to reduce ferric ions were observed in rats fed both diets contaning linoleic acid-rich sunflower oil compared to animals fed the corresponding energy from other fats. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of plasma was lower in high-lard and high-FO fed rats compared to the corresponding low-fat diets, and the lowest in low-FO fed rats among low-fat fed animals.We presume from our results that linoleic acid may have dual effect, prooxidative in blood cells but maintaining total antioxidant plasma ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell-to-Cell Communication, Medical University of Lodz, Lodz 92-215, Poland.

ABSTRACT
This study was performed to examine the effect of different fat sources, lard, sunflower oil (SO), and fish oil (FO) in high-fat and low-fat diet on reactive oxygen species generation by blood phagocytes, glutathione redox status in erythrocytes, and total plasma antioxidant ability in rats. Whole blood chemiluminescence (CL) did not differ between three low-fat fed groups. However, baseline and phorbol myristate acetate (PMA)-stimulated CL in blood of high-lard fed rats were lower than in low-lard and high-SO fed animals. Phagocyte-stimulated oxidative burst was higher in rats fed high-SO diet than in those fed low-SO and high-FO diets. The highest level of oxidize glutathione (GSSH), the lowest reduce glutathione (GSH)/GSSG ratio in erythrocytes, and the highest plasma activity to reduce ferric ions were observed in rats fed both diets contaning linoleic acid-rich sunflower oil compared to animals fed the corresponding energy from other fats. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of plasma was lower in high-lard and high-FO fed rats compared to the corresponding low-fat diets, and the lowest in low-FO fed rats among low-fat fed animals. We presume from our results that linoleic acid may have dual effect, prooxidative in blood cells but maintaining total antioxidant plasma ability.

No MeSH data available.