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Applications of electron spin resonance spectrometry for reactive oxygen species and reactive nitrogen species research.

Kohno M - J Clin Biochem Nutr (2010)

Bottom Line: The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals.This method is now widely used as one of the most powerful tools for free radical studies.In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

View Article: PubMed Central - PubMed

Affiliation: New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.

ABSTRACT
Electron spin resonance (ESR) spectroscopy has been widely applied in the research of biological free radicals for quantitative and qualitative analyses of reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals. This method is now widely used as one of the most powerful tools for free radical studies. In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

No MeSH data available.


Chemical reactions between Fe-bisMGD or Fe-bisDTCS, with NO: novel methods for the quantitative and qualitative assessment of NO at room temperature. (a) and (b) show the structures of MGD and DTCS, respectively, and (c) shows a representative ESR spectrum of MGD2-Fe-NO.
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Figure 6: Chemical reactions between Fe-bisMGD or Fe-bisDTCS, with NO: novel methods for the quantitative and qualitative assessment of NO at room temperature. (a) and (b) show the structures of MGD and DTCS, respectively, and (c) shows a representative ESR spectrum of MGD2-Fe-NO.

Mentions: More recently, another method, as summarized in Fig. 6, has been developed to measure NO using the reagents MGD [N-(dithiocarbamoyl)-N-methyl-D-glucamine, sodium salt] and DTCS [N-(dithiocarboxy)sarcosine, disodium salt, dehydrate], which possess an iron ion [44]. Unlike the NO-bound hemoglobin method, the MGD/DTCS method can be performed at room temperature and in an aqueous solution.


Applications of electron spin resonance spectrometry for reactive oxygen species and reactive nitrogen species research.

Kohno M - J Clin Biochem Nutr (2010)

Chemical reactions between Fe-bisMGD or Fe-bisDTCS, with NO: novel methods for the quantitative and qualitative assessment of NO at room temperature. (a) and (b) show the structures of MGD and DTCS, respectively, and (c) shows a representative ESR spectrum of MGD2-Fe-NO.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2901757&req=5

Figure 6: Chemical reactions between Fe-bisMGD or Fe-bisDTCS, with NO: novel methods for the quantitative and qualitative assessment of NO at room temperature. (a) and (b) show the structures of MGD and DTCS, respectively, and (c) shows a representative ESR spectrum of MGD2-Fe-NO.
Mentions: More recently, another method, as summarized in Fig. 6, has been developed to measure NO using the reagents MGD [N-(dithiocarbamoyl)-N-methyl-D-glucamine, sodium salt] and DTCS [N-(dithiocarboxy)sarcosine, disodium salt, dehydrate], which possess an iron ion [44]. Unlike the NO-bound hemoglobin method, the MGD/DTCS method can be performed at room temperature and in an aqueous solution.

Bottom Line: The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals.This method is now widely used as one of the most powerful tools for free radical studies.In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

View Article: PubMed Central - PubMed

Affiliation: New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.

ABSTRACT
Electron spin resonance (ESR) spectroscopy has been widely applied in the research of biological free radicals for quantitative and qualitative analyses of reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals. This method is now widely used as one of the most powerful tools for free radical studies. In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

No MeSH data available.