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Applications of electron spin resonance spectrometry for reactive oxygen species and reactive nitrogen species research.

Kohno M - J Clin Biochem Nutr (2010)

Bottom Line: The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals.This method is now widely used as one of the most powerful tools for free radical studies.In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

View Article: PubMed Central - PubMed

Affiliation: New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.

ABSTRACT
Electron spin resonance (ESR) spectroscopy has been widely applied in the research of biological free radicals for quantitative and qualitative analyses of reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals. This method is now widely used as one of the most powerful tools for free radical studies. In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

No MeSH data available.


Chemical reaction between the stable free radical carboxyl-PTIO and NO, which is used for the quantitative and qualitative assessment of NO.
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Related In: Results  -  Collection


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Figure 5: Chemical reaction between the stable free radical carboxyl-PTIO and NO, which is used for the quantitative and qualitative assessment of NO.

Mentions: The use of ESR spectrometry to measure NO was first reported in 1993 [6]. As shown in Fig. 5, a stable free radical, carboxyl-PTIO reacts with NO, and carboxyl-PTI is formed. The Griess, electrode and chemiluminescence methods, for example, are the most widely used conventional methods [38–42]. The PTIO method offers higher selectivity and higher sensitivity compared with these conventional methods.


Applications of electron spin resonance spectrometry for reactive oxygen species and reactive nitrogen species research.

Kohno M - J Clin Biochem Nutr (2010)

Chemical reaction between the stable free radical carboxyl-PTIO and NO, which is used for the quantitative and qualitative assessment of NO.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2901757&req=5

Figure 5: Chemical reaction between the stable free radical carboxyl-PTIO and NO, which is used for the quantitative and qualitative assessment of NO.
Mentions: The use of ESR spectrometry to measure NO was first reported in 1993 [6]. As shown in Fig. 5, a stable free radical, carboxyl-PTIO reacts with NO, and carboxyl-PTI is formed. The Griess, electrode and chemiluminescence methods, for example, are the most widely used conventional methods [38–42]. The PTIO method offers higher selectivity and higher sensitivity compared with these conventional methods.

Bottom Line: The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals.This method is now widely used as one of the most powerful tools for free radical studies.In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

View Article: PubMed Central - PubMed

Affiliation: New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.

ABSTRACT
Electron spin resonance (ESR) spectroscopy has been widely applied in the research of biological free radicals for quantitative and qualitative analyses of reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals. This method is now widely used as one of the most powerful tools for free radical studies. In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

No MeSH data available.