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Applications of electron spin resonance spectrometry for reactive oxygen species and reactive nitrogen species research.

Kohno M - J Clin Biochem Nutr (2010)

Bottom Line: The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals.This method is now widely used as one of the most powerful tools for free radical studies.In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

View Article: PubMed Central - PubMed

Affiliation: New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.

ABSTRACT
Electron spin resonance (ESR) spectroscopy has been widely applied in the research of biological free radicals for quantitative and qualitative analyses of reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals. This method is now widely used as one of the most powerful tools for free radical studies. In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

No MeSH data available.


Xanthine oxidase and NADPH oxidase: representative enzymes that contribute to the production of O2•− in biological systems.
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Figure 3: Xanthine oxidase and NADPH oxidase: representative enzymes that contribute to the production of O2•− in biological systems.

Mentions: ESR spin-trapping methods have also been applied to measure SOD and SOD-like activities. The typical enzyme reactions for the generation of O2•− are shown in Fig. 3, and both reaction systems have been used to evaluate the superoxide scavenging activity of many natural products [13, 18–29]. These methods also can be applied for kinetic analyses [30, 31]. For example, the DMPO spin-trapping method has been used to determine the enzyme reaction velocity of xanthine oxidizing enzyme (XOD) and an NADPH oxidizing enzyme [18, 32].


Applications of electron spin resonance spectrometry for reactive oxygen species and reactive nitrogen species research.

Kohno M - J Clin Biochem Nutr (2010)

Xanthine oxidase and NADPH oxidase: representative enzymes that contribute to the production of O2•− in biological systems.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2901757&req=5

Figure 3: Xanthine oxidase and NADPH oxidase: representative enzymes that contribute to the production of O2•− in biological systems.
Mentions: ESR spin-trapping methods have also been applied to measure SOD and SOD-like activities. The typical enzyme reactions for the generation of O2•− are shown in Fig. 3, and both reaction systems have been used to evaluate the superoxide scavenging activity of many natural products [13, 18–29]. These methods also can be applied for kinetic analyses [30, 31]. For example, the DMPO spin-trapping method has been used to determine the enzyme reaction velocity of xanthine oxidizing enzyme (XOD) and an NADPH oxidizing enzyme [18, 32].

Bottom Line: The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals.This method is now widely used as one of the most powerful tools for free radical studies.In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

View Article: PubMed Central - PubMed

Affiliation: New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.

ABSTRACT
Electron spin resonance (ESR) spectroscopy has been widely applied in the research of biological free radicals for quantitative and qualitative analyses of reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ESR spin-trapping method was developed in the early 1970s and enabled the analysis of short-lived free radicals. This method is now widely used as one of the most powerful tools for free radical studies. In this report, some of the studies that applied ESR for the measurement of ROS and RNS during oxidative stress are discussed.

No MeSH data available.