Limits...
The relationship between the presence of chromosomal instability and prognosis of squamous cell carcinoma of the lung: fluorescence in situ hybridization analysis of paraffin-embedded tissue from 47 Korean patients.

Yoo JW, Seo KW, Jang SJ, Oh YM, Shim TS, Kim WS, Lee DS, Lee SD, Choi CM - J. Korean Med. Sci. (2010)

Bottom Line: Survival rates were compared in terms of age, T factor, N factor, CIN, and smoking status.In multivariate analysis, the presence of CIN was a predictive factor for survival.CIN-positive based on FISH can be prognostic factor of lung SCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary and Critical Care Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.

ABSTRACT
To evaluate the prognostic importance of chromosomal instability (CIN) in squamous cell carcinoma (SCC) of the lung, the relationship between CIN detected by fluorescence in situ hybridization (FISH) and survival in SCC patients was examined. Forty-seven surgical specimens of lung SCC were analyzed. To identify tumors with CIN, p16 and multi-target DNA FISH assays for c-myc, chromosome 6, EGFR, and chromosome 5 (LAVysion, Vysis) were performed on nuclei extracted from paraffin-embedded tumor tissues. Survival rates were compared in terms of age, T factor, N factor, CIN, and smoking status. A sample was defined as CIN-positive if at least four of the five chromosomes were positive. Among the 47 specimens, 9 (19%) were CIN-positive. The overall survival rate was 66%. Overall survival rates were estimated as 33.3% for CIN-positive patients and 76.7% for CIN-negative patients (Hazard ratio 3.47; 95% Confidence interval, 1.25-9.67; P=0.017). In multivariate analysis, the presence of CIN was a predictive factor for survival. CIN-positive based on FISH can be prognostic factor of lung SCC.

Show MeSH

Related in: MedlinePlus

Overall survival curve according to CIN status (P=0.013).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2877246&req=5

Figure 2: Overall survival curve according to CIN status (P=0.013).

Mentions: The median age of both CIN-positive group and CIN-negative group was 59 yr. The level of lymph node involvement, tumor stage, and smoking history did not differ significantly between the groups (Table 1). The frequencies of chromosomal aneusomy in SCC specimens are summarized in Table 2. Nine cases met our criteria for CIN positive. As of November 2008, 16 patients had died. The mean survival duration was 52±8 months (95% Confidence interval (CI), 37-67) for CIN-positive group and 71±3 months (95% CI, 65-78). The overall survival rate was 66%. Overall survival rates were estimated as 33.3% for CIN-positive patients and 76.7% for CIN-negative patients (Hazard ratio (HR) 3.47; 95% CI, 1.25-9.67; P=0.017). Multivariate analysis using Cox proportional hazards models was used to compare survival between CIN-positive and CIN-negative patients. In relation to CIN-negative patients and after adjusting for sex, pathologic nodal staging, tumor staging, age, and smoking history, the presence of CIN was associated with survival (HR, 3.47; 95% CI, 1.25-9.67; P=0.017) (Table 3). The Kaplan-Meier survival curves constructed according to CIN status after resection of lung SCC are shown in Fig. 2 (P=0.013).


The relationship between the presence of chromosomal instability and prognosis of squamous cell carcinoma of the lung: fluorescence in situ hybridization analysis of paraffin-embedded tissue from 47 Korean patients.

Yoo JW, Seo KW, Jang SJ, Oh YM, Shim TS, Kim WS, Lee DS, Lee SD, Choi CM - J. Korean Med. Sci. (2010)

Overall survival curve according to CIN status (P=0.013).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2877246&req=5

Figure 2: Overall survival curve according to CIN status (P=0.013).
Mentions: The median age of both CIN-positive group and CIN-negative group was 59 yr. The level of lymph node involvement, tumor stage, and smoking history did not differ significantly between the groups (Table 1). The frequencies of chromosomal aneusomy in SCC specimens are summarized in Table 2. Nine cases met our criteria for CIN positive. As of November 2008, 16 patients had died. The mean survival duration was 52±8 months (95% Confidence interval (CI), 37-67) for CIN-positive group and 71±3 months (95% CI, 65-78). The overall survival rate was 66%. Overall survival rates were estimated as 33.3% for CIN-positive patients and 76.7% for CIN-negative patients (Hazard ratio (HR) 3.47; 95% CI, 1.25-9.67; P=0.017). Multivariate analysis using Cox proportional hazards models was used to compare survival between CIN-positive and CIN-negative patients. In relation to CIN-negative patients and after adjusting for sex, pathologic nodal staging, tumor staging, age, and smoking history, the presence of CIN was associated with survival (HR, 3.47; 95% CI, 1.25-9.67; P=0.017) (Table 3). The Kaplan-Meier survival curves constructed according to CIN status after resection of lung SCC are shown in Fig. 2 (P=0.013).

Bottom Line: Survival rates were compared in terms of age, T factor, N factor, CIN, and smoking status.In multivariate analysis, the presence of CIN was a predictive factor for survival.CIN-positive based on FISH can be prognostic factor of lung SCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary and Critical Care Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.

ABSTRACT
To evaluate the prognostic importance of chromosomal instability (CIN) in squamous cell carcinoma (SCC) of the lung, the relationship between CIN detected by fluorescence in situ hybridization (FISH) and survival in SCC patients was examined. Forty-seven surgical specimens of lung SCC were analyzed. To identify tumors with CIN, p16 and multi-target DNA FISH assays for c-myc, chromosome 6, EGFR, and chromosome 5 (LAVysion, Vysis) were performed on nuclei extracted from paraffin-embedded tumor tissues. Survival rates were compared in terms of age, T factor, N factor, CIN, and smoking status. A sample was defined as CIN-positive if at least four of the five chromosomes were positive. Among the 47 specimens, 9 (19%) were CIN-positive. The overall survival rate was 66%. Overall survival rates were estimated as 33.3% for CIN-positive patients and 76.7% for CIN-negative patients (Hazard ratio 3.47; 95% Confidence interval, 1.25-9.67; P=0.017). In multivariate analysis, the presence of CIN was a predictive factor for survival. CIN-positive based on FISH can be prognostic factor of lung SCC.

Show MeSH
Related in: MedlinePlus