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Enhancement of gastric ulcer healing and angiogenesis by cochinchina Momordica seed extract in rats.

Kang JM, Kim N, Kim B, Kim JH, Lee BY, Park JH, Lee MK, Lee HS, Kim JS, Jung HC, Song IS - J. Korean Med. Sci. (2010)

Bottom Line: The treatment with SK-MS10 for 7 and 14 days significantly accelerated ulcer healing and increased the expression of mRNA (at day 7) as well as VEGF protein (at day 14) compared to the vehicle-treated rats.The MVD for factor VIII was also higher in the SK-MS10 treatment group compared to the vehicle-treated rats; however, these differences were not statistically significant.These results suggest that SK-MS10 treatment accelerates the healing of gastric ulcers via upregulation of VEGF and angiogenesis in an acetic acid rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT
Cochinchina momordica seed is the dried ripe seed of Momordica cochinchinensis, a perennial vine. The antiulcer effect of an extract from cochinchina momordica seeds (SK-MS10) was evaluated in a rat model of acetic acid-induced gastric ulcers. Gastric ulcers were produced by subserosal injection of acetic acid. SK-MS10 (200 mg/kg) or vehicle was administered orally once per day for 14 days after the acetic acid injection. The stomach was removed and the ulcer size measured at day 7 and 14 of the treatment. Expression of vascular endothelial growth factor (VEGF) was assessed by real-time RT-PCR and Western blot analysis. In addition, the microvasculature density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry. The treatment with SK-MS10 for 7 and 14 days significantly accelerated ulcer healing and increased the expression of mRNA (at day 7) as well as VEGF protein (at day 14) compared to the vehicle-treated rats. The MVD for factor VIII was also higher in the SK-MS10 treatment group compared to the vehicle-treated rats; however, these differences were not statistically significant. These results suggest that SK-MS10 treatment accelerates the healing of gastric ulcers via upregulation of VEGF and angiogenesis in an acetic acid rat model.

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Effects of SK-MS10 on the expression of VEGF. (A) The relative mRNA expression of VEGF. (B) Western blot analysis for VEGF. Results are mean±SE in 6 animals per group. *P value <0.05 when compared with the vehicle treated group.
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Figure 3: Effects of SK-MS10 on the expression of VEGF. (A) The relative mRNA expression of VEGF. (B) Western blot analysis for VEGF. Results are mean±SE in 6 animals per group. *P value <0.05 when compared with the vehicle treated group.

Mentions: The mRNA expression of VEGF after 7 days of SK-MS10 treatment was significantly higher than in the vehicle treated group (0.7 vs. 5.4 for VEGF/β-actin, respectively Fig. 3A). However, the mRNA expression of VEGF after 14 days of SK-MS10 treatment was not significantly different from the vehicle treated group (Fig. 3A). On Western blot analysis, the expression of VEGF proteins 14 days after SK-MS10 treatment was significantly higher than in the vehicle group (2.7 vs. 6.0 for VEGF/β-actin, respectively Fig. 3B). However, the protein expression of VEGF 7 days after SK-MS10 treatment was not significantly different from the vehicle treated group (Fig. 3B).


Enhancement of gastric ulcer healing and angiogenesis by cochinchina Momordica seed extract in rats.

Kang JM, Kim N, Kim B, Kim JH, Lee BY, Park JH, Lee MK, Lee HS, Kim JS, Jung HC, Song IS - J. Korean Med. Sci. (2010)

Effects of SK-MS10 on the expression of VEGF. (A) The relative mRNA expression of VEGF. (B) Western blot analysis for VEGF. Results are mean±SE in 6 animals per group. *P value <0.05 when compared with the vehicle treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2877244&req=5

Figure 3: Effects of SK-MS10 on the expression of VEGF. (A) The relative mRNA expression of VEGF. (B) Western blot analysis for VEGF. Results are mean±SE in 6 animals per group. *P value <0.05 when compared with the vehicle treated group.
Mentions: The mRNA expression of VEGF after 7 days of SK-MS10 treatment was significantly higher than in the vehicle treated group (0.7 vs. 5.4 for VEGF/β-actin, respectively Fig. 3A). However, the mRNA expression of VEGF after 14 days of SK-MS10 treatment was not significantly different from the vehicle treated group (Fig. 3A). On Western blot analysis, the expression of VEGF proteins 14 days after SK-MS10 treatment was significantly higher than in the vehicle group (2.7 vs. 6.0 for VEGF/β-actin, respectively Fig. 3B). However, the protein expression of VEGF 7 days after SK-MS10 treatment was not significantly different from the vehicle treated group (Fig. 3B).

Bottom Line: The treatment with SK-MS10 for 7 and 14 days significantly accelerated ulcer healing and increased the expression of mRNA (at day 7) as well as VEGF protein (at day 14) compared to the vehicle-treated rats.The MVD for factor VIII was also higher in the SK-MS10 treatment group compared to the vehicle-treated rats; however, these differences were not statistically significant.These results suggest that SK-MS10 treatment accelerates the healing of gastric ulcers via upregulation of VEGF and angiogenesis in an acetic acid rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT
Cochinchina momordica seed is the dried ripe seed of Momordica cochinchinensis, a perennial vine. The antiulcer effect of an extract from cochinchina momordica seeds (SK-MS10) was evaluated in a rat model of acetic acid-induced gastric ulcers. Gastric ulcers were produced by subserosal injection of acetic acid. SK-MS10 (200 mg/kg) or vehicle was administered orally once per day for 14 days after the acetic acid injection. The stomach was removed and the ulcer size measured at day 7 and 14 of the treatment. Expression of vascular endothelial growth factor (VEGF) was assessed by real-time RT-PCR and Western blot analysis. In addition, the microvasculature density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry. The treatment with SK-MS10 for 7 and 14 days significantly accelerated ulcer healing and increased the expression of mRNA (at day 7) as well as VEGF protein (at day 14) compared to the vehicle-treated rats. The MVD for factor VIII was also higher in the SK-MS10 treatment group compared to the vehicle-treated rats; however, these differences were not statistically significant. These results suggest that SK-MS10 treatment accelerates the healing of gastric ulcers via upregulation of VEGF and angiogenesis in an acetic acid rat model.

Show MeSH
Related in: MedlinePlus