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Prevention of UV-induced skin damages by 11,14,17-eicosatrienoic acid in hairless mice in vivo.

Jin XJ, Kim EJ, Oh IK, Kim YK, Park CH, Chung JH - J. Korean Med. Sci. (2010)

Bottom Line: Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes.However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood.We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul Nationa University College of Medicine, Seoul, Korea.

ABSTRACT
Polyunsaturated fatty acids (PUFAs) are known to play important roles in various physiological and pathological processes. Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes. However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood. In this study, we investigated the cutaneous photoprotective effects of ETA in hairless mice in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA, or 1% ETA once a day for 3 successive days after one time UV irradiation (200 mJ/cm(2)) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function. In addition, ETA suppressed the expression of IL-1beta, COX-2, and MMP-13 induced by UV irradiation. Our results show that the topical application of ETA protects against UV-induced skin damage in hairless mice and suggest that ETA can be a potential agent for preventing and/or treating UV-induced inflammation and photoaging.

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Topical application of ETA prevents UV-induced interleukin-1beta (IL-1β) and cyclooxygenase-2 (COX-2) expressions in hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Expression of IL-1β mRNA was determined by quantitative real-time RT-PCR. (B) Expression of IL-1β protein was analyzed by ELISA. (C) Expression of COX-2 mRNA was detected by quantitative real-time RT-PCR. (D) Expression of COX-2 protein was detected by Western blot analysis. Results are expressed as fold change. Values are mean±SEM (n=8).*P<0.01, †P<0.001 vs. control (CON) vehicle group; ‡P<0.05, §P<0.01 ∥P<0.001 vs. UV-irradiated (UV) vehicle group.
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Figure 3: Topical application of ETA prevents UV-induced interleukin-1beta (IL-1β) and cyclooxygenase-2 (COX-2) expressions in hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Expression of IL-1β mRNA was determined by quantitative real-time RT-PCR. (B) Expression of IL-1β protein was analyzed by ELISA. (C) Expression of COX-2 mRNA was detected by quantitative real-time RT-PCR. (D) Expression of COX-2 protein was detected by Western blot analysis. Results are expressed as fold change. Values are mean±SEM (n=8).*P<0.01, †P<0.001 vs. control (CON) vehicle group; ‡P<0.05, §P<0.01 ∥P<0.001 vs. UV-irradiated (UV) vehicle group.

Mentions: To understand the molecular mechanisms of the ETA-mediated inhibition of skin thickening and inflammation in UV-irradiated hairless mice, we investigated the effects of ETA on IL-1β and COX-2 that play important roles in inflammation. We examined whether ETA could affect UV-induced IL-1β expression. The mRNA and protein levels of IL-1β were detected by quantitative real-time PCR (Fig. 3A) and ELISA (Fig. 3B) respectively. Both mRNA and protein levels of IL-1β were increased dramatically by UV and the UV-induced IL-1β mRNA and protein levels were significantly decreased by ETA, in a dose-dependent manner (Fig. 3A, B).


Prevention of UV-induced skin damages by 11,14,17-eicosatrienoic acid in hairless mice in vivo.

Jin XJ, Kim EJ, Oh IK, Kim YK, Park CH, Chung JH - J. Korean Med. Sci. (2010)

Topical application of ETA prevents UV-induced interleukin-1beta (IL-1β) and cyclooxygenase-2 (COX-2) expressions in hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Expression of IL-1β mRNA was determined by quantitative real-time RT-PCR. (B) Expression of IL-1β protein was analyzed by ELISA. (C) Expression of COX-2 mRNA was detected by quantitative real-time RT-PCR. (D) Expression of COX-2 protein was detected by Western blot analysis. Results are expressed as fold change. Values are mean±SEM (n=8).*P<0.01, †P<0.001 vs. control (CON) vehicle group; ‡P<0.05, §P<0.01 ∥P<0.001 vs. UV-irradiated (UV) vehicle group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 3: Topical application of ETA prevents UV-induced interleukin-1beta (IL-1β) and cyclooxygenase-2 (COX-2) expressions in hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Expression of IL-1β mRNA was determined by quantitative real-time RT-PCR. (B) Expression of IL-1β protein was analyzed by ELISA. (C) Expression of COX-2 mRNA was detected by quantitative real-time RT-PCR. (D) Expression of COX-2 protein was detected by Western blot analysis. Results are expressed as fold change. Values are mean±SEM (n=8).*P<0.01, †P<0.001 vs. control (CON) vehicle group; ‡P<0.05, §P<0.01 ∥P<0.001 vs. UV-irradiated (UV) vehicle group.
Mentions: To understand the molecular mechanisms of the ETA-mediated inhibition of skin thickening and inflammation in UV-irradiated hairless mice, we investigated the effects of ETA on IL-1β and COX-2 that play important roles in inflammation. We examined whether ETA could affect UV-induced IL-1β expression. The mRNA and protein levels of IL-1β were detected by quantitative real-time PCR (Fig. 3A) and ELISA (Fig. 3B) respectively. Both mRNA and protein levels of IL-1β were increased dramatically by UV and the UV-induced IL-1β mRNA and protein levels were significantly decreased by ETA, in a dose-dependent manner (Fig. 3A, B).

Bottom Line: Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes.However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood.We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul Nationa University College of Medicine, Seoul, Korea.

ABSTRACT
Polyunsaturated fatty acids (PUFAs) are known to play important roles in various physiological and pathological processes. Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes. However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood. In this study, we investigated the cutaneous photoprotective effects of ETA in hairless mice in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA, or 1% ETA once a day for 3 successive days after one time UV irradiation (200 mJ/cm(2)) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function. In addition, ETA suppressed the expression of IL-1beta, COX-2, and MMP-13 induced by UV irradiation. Our results show that the topical application of ETA protects against UV-induced skin damage in hairless mice and suggest that ETA can be a potential agent for preventing and/or treating UV-induced inflammation and photoaging.

Show MeSH
Related in: MedlinePlus