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Prevention of UV-induced skin damages by 11,14,17-eicosatrienoic acid in hairless mice in vivo.

Jin XJ, Kim EJ, Oh IK, Kim YK, Park CH, Chung JH - J. Korean Med. Sci. (2010)

Bottom Line: Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes.However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood.We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul Nationa University College of Medicine, Seoul, Korea.

ABSTRACT
Polyunsaturated fatty acids (PUFAs) are known to play important roles in various physiological and pathological processes. Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes. However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood. In this study, we investigated the cutaneous photoprotective effects of ETA in hairless mice in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA, or 1% ETA once a day for 3 successive days after one time UV irradiation (200 mJ/cm(2)) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function. In addition, ETA suppressed the expression of IL-1beta, COX-2, and MMP-13 induced by UV irradiation. Our results show that the topical application of ETA protects against UV-induced skin damage in hairless mice and suggest that ETA can be a potential agent for preventing and/or treating UV-induced inflammation and photoaging.

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Topical application of ETA increases stratum corneum hydration and decreases transepidermal water loss (TEWL) in control and UV-irradiated hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Stratum corneum hydration was dramatically decreased after UV irradiation. Stratum corneum hydration was increased by topical application of ETA both in normal and UV-irradiated hairless mice. (B) TEWL was dramatically increased after UV irradiation. Topical application of ETA decreased TEWL both in control and UV-irradiated hairless mice. Values are mean±SEM (n=8).*P<0.05; ‡P<0.001 vs. control (CON) vehicle group; ‡P<0.05 vs. UV-irradiated (UV) vehicle group.
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Figure 2: Topical application of ETA increases stratum corneum hydration and decreases transepidermal water loss (TEWL) in control and UV-irradiated hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Stratum corneum hydration was dramatically decreased after UV irradiation. Stratum corneum hydration was increased by topical application of ETA both in normal and UV-irradiated hairless mice. (B) TEWL was dramatically increased after UV irradiation. Topical application of ETA decreased TEWL both in control and UV-irradiated hairless mice. Values are mean±SEM (n=8).*P<0.05; ‡P<0.001 vs. control (CON) vehicle group; ‡P<0.05 vs. UV-irradiated (UV) vehicle group.

Mentions: We also anticipated whether ETA has other advantages on skin, such as skin barrier function. Stratum corneum hydration and TEWL reflect cutaneous barrier function directly. To study the effects of ETA on cutaneous barrier function, we measured stratum corneum hydration and TEWL in control and UV-irradiated mice, respectively. We confirmed that UV decreased stratum corneum hydration and increased TEWL significantly. Stratum corneum hydration (Fig. 2A) was increased whereas TEWL (Fig. 2B) was decreased by ETA, in a dose-dependent manner, in both control and UV-irradiated groups, respectively. These results suggest that topical treatment with ETA improves skin barrier function in hairless mice in vivo.


Prevention of UV-induced skin damages by 11,14,17-eicosatrienoic acid in hairless mice in vivo.

Jin XJ, Kim EJ, Oh IK, Kim YK, Park CH, Chung JH - J. Korean Med. Sci. (2010)

Topical application of ETA increases stratum corneum hydration and decreases transepidermal water loss (TEWL) in control and UV-irradiated hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Stratum corneum hydration was dramatically decreased after UV irradiation. Stratum corneum hydration was increased by topical application of ETA both in normal and UV-irradiated hairless mice. (B) TEWL was dramatically increased after UV irradiation. Topical application of ETA decreased TEWL both in control and UV-irradiated hairless mice. Values are mean±SEM (n=8).*P<0.05; ‡P<0.001 vs. control (CON) vehicle group; ‡P<0.05 vs. UV-irradiated (UV) vehicle group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2877234&req=5

Figure 2: Topical application of ETA increases stratum corneum hydration and decreases transepidermal water loss (TEWL) in control and UV-irradiated hairless mouse skin in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA or 1% ETA once a day for 3 successive days after one time irradiation of UV (200 mJ/cm2) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). (A) Stratum corneum hydration was dramatically decreased after UV irradiation. Stratum corneum hydration was increased by topical application of ETA both in normal and UV-irradiated hairless mice. (B) TEWL was dramatically increased after UV irradiation. Topical application of ETA decreased TEWL both in control and UV-irradiated hairless mice. Values are mean±SEM (n=8).*P<0.05; ‡P<0.001 vs. control (CON) vehicle group; ‡P<0.05 vs. UV-irradiated (UV) vehicle group.
Mentions: We also anticipated whether ETA has other advantages on skin, such as skin barrier function. Stratum corneum hydration and TEWL reflect cutaneous barrier function directly. To study the effects of ETA on cutaneous barrier function, we measured stratum corneum hydration and TEWL in control and UV-irradiated mice, respectively. We confirmed that UV decreased stratum corneum hydration and increased TEWL significantly. Stratum corneum hydration (Fig. 2A) was increased whereas TEWL (Fig. 2B) was decreased by ETA, in a dose-dependent manner, in both control and UV-irradiated groups, respectively. These results suggest that topical treatment with ETA improves skin barrier function in hairless mice in vivo.

Bottom Line: Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes.However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood.We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Seoul Nationa University College of Medicine, Seoul, Korea.

ABSTRACT
Polyunsaturated fatty acids (PUFAs) are known to play important roles in various physiological and pathological processes. Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes. However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood. In this study, we investigated the cutaneous photoprotective effects of ETA in hairless mice in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA, or 1% ETA once a day for 3 successive days after one time UV irradiation (200 mJ/cm(2)) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function. In addition, ETA suppressed the expression of IL-1beta, COX-2, and MMP-13 induced by UV irradiation. Our results show that the topical application of ETA protects against UV-induced skin damage in hairless mice and suggest that ETA can be a potential agent for preventing and/or treating UV-induced inflammation and photoaging.

Show MeSH
Related in: MedlinePlus