Limits...
Increased seizure susceptibility and up-regulation of nNOS expression in hippocampus following recurrent early-life seizures in rats.

Kim DK - J. Korean Med. Sci. (2010)

Bottom Line: Changes in nNOS expression were determined by quantitative immunoblotting on day 50.Up-regulation of nNOS expression following early-life recurrent seizures was observed on day 50.In conclusion, these data suggested that recurrent early-life seizures had the long-term effects on seizure susceptibility late in life and up-regulatory nNOS expression on the hippocampus during brain development, and nNOS appeared to contribute to the persistent changes in seizure susceptibility, and epileptogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Dongguk University College of Medicine, Gyeongju, Korea. pedepi@hotmail.com

ABSTRACT
This study aimed to determine the long-term change of seizure susceptibility and the role of nNOS on brain development following recurrent early-life seizures in rats. Video-EEG recordings were conducted between postnatal days 50 and 60. Alterations in seizure susceptibility were assayed on day 22 or 50 using the flurothyl method. Changes in nNOS expression were determined by quantitative immunoblotting on day 50. On average, rats had 8.4+/-2.7 seizures during 10 daily 1 hr behavioral monitoring sessions. As adults (days 50-60), all rats displayed interictal spikes in the hippocampus and/or overlying cortex. Brief electrographic seizures were recorded in only one of five animals. Rats appeared to progress from a period of marked seizure susceptibility (day 22) to one of lessened seizure susceptibility (day 50). Up-regulation of nNOS expression following early-life recurrent seizures was observed on day 50. In conclusion, these data suggested that recurrent early-life seizures had the long-term effects on seizure susceptibility late in life and up-regulatory nNOS expression on the hippocampus during brain development, and nNOS appeared to contribute to the persistent changes in seizure susceptibility, and epileptogenesis.

Show MeSH

Related in: MedlinePlus

Expression of nNOS level in the hippocampal subfield, CA1, CA3, and dentate gyrus of the right hippocampus on day 45 following recurrent seizures on day 10. Distinctively, the significant up-regulation of nNOS is revealed in the CA1 and CA3 subfields of the hippocampus. However, there is statistically no change in nNOS expression level in the dentate gyrus.NC, normal control; SC, saline control; TNTX, tetanus toxin injected; DG, dentate gyrus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2877220&req=5

Figure 4: Expression of nNOS level in the hippocampal subfield, CA1, CA3, and dentate gyrus of the right hippocampus on day 45 following recurrent seizures on day 10. Distinctively, the significant up-regulation of nNOS is revealed in the CA1 and CA3 subfields of the hippocampus. However, there is statistically no change in nNOS expression level in the dentate gyrus.NC, normal control; SC, saline control; TNTX, tetanus toxin injected; DG, dentate gyrus.

Mentions: The results are shown in Fig. 4. On P50, hippocampal slices were microdissected into CA1 and, CA3 subfields and the dentate gyrus, and assayed separately. The results were revealed that area CA1 displayed a 300% increase in nNOS expression (TNTX: 78.7±3.77, saline control: 26.2±3.20), and CA3 displayed an 164% increase (TNTX: 79±3.80, saline control: 48.2±3.97). However, there was statistically no change in nNOS expression level in the dentate gyrus (TNTX: 57±3.01, saline control: 47±2.98).


Increased seizure susceptibility and up-regulation of nNOS expression in hippocampus following recurrent early-life seizures in rats.

Kim DK - J. Korean Med. Sci. (2010)

Expression of nNOS level in the hippocampal subfield, CA1, CA3, and dentate gyrus of the right hippocampus on day 45 following recurrent seizures on day 10. Distinctively, the significant up-regulation of nNOS is revealed in the CA1 and CA3 subfields of the hippocampus. However, there is statistically no change in nNOS expression level in the dentate gyrus.NC, normal control; SC, saline control; TNTX, tetanus toxin injected; DG, dentate gyrus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2877220&req=5

Figure 4: Expression of nNOS level in the hippocampal subfield, CA1, CA3, and dentate gyrus of the right hippocampus on day 45 following recurrent seizures on day 10. Distinctively, the significant up-regulation of nNOS is revealed in the CA1 and CA3 subfields of the hippocampus. However, there is statistically no change in nNOS expression level in the dentate gyrus.NC, normal control; SC, saline control; TNTX, tetanus toxin injected; DG, dentate gyrus.
Mentions: The results are shown in Fig. 4. On P50, hippocampal slices were microdissected into CA1 and, CA3 subfields and the dentate gyrus, and assayed separately. The results were revealed that area CA1 displayed a 300% increase in nNOS expression (TNTX: 78.7±3.77, saline control: 26.2±3.20), and CA3 displayed an 164% increase (TNTX: 79±3.80, saline control: 48.2±3.97). However, there was statistically no change in nNOS expression level in the dentate gyrus (TNTX: 57±3.01, saline control: 47±2.98).

Bottom Line: Changes in nNOS expression were determined by quantitative immunoblotting on day 50.Up-regulation of nNOS expression following early-life recurrent seizures was observed on day 50.In conclusion, these data suggested that recurrent early-life seizures had the long-term effects on seizure susceptibility late in life and up-regulatory nNOS expression on the hippocampus during brain development, and nNOS appeared to contribute to the persistent changes in seizure susceptibility, and epileptogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Dongguk University College of Medicine, Gyeongju, Korea. pedepi@hotmail.com

ABSTRACT
This study aimed to determine the long-term change of seizure susceptibility and the role of nNOS on brain development following recurrent early-life seizures in rats. Video-EEG recordings were conducted between postnatal days 50 and 60. Alterations in seizure susceptibility were assayed on day 22 or 50 using the flurothyl method. Changes in nNOS expression were determined by quantitative immunoblotting on day 50. On average, rats had 8.4+/-2.7 seizures during 10 daily 1 hr behavioral monitoring sessions. As adults (days 50-60), all rats displayed interictal spikes in the hippocampus and/or overlying cortex. Brief electrographic seizures were recorded in only one of five animals. Rats appeared to progress from a period of marked seizure susceptibility (day 22) to one of lessened seizure susceptibility (day 50). Up-regulation of nNOS expression following early-life recurrent seizures was observed on day 50. In conclusion, these data suggested that recurrent early-life seizures had the long-term effects on seizure susceptibility late in life and up-regulatory nNOS expression on the hippocampus during brain development, and nNOS appeared to contribute to the persistent changes in seizure susceptibility, and epileptogenesis.

Show MeSH
Related in: MedlinePlus