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Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report.

Zhang K, Shang Y, Jin Y, Wang G, Zheng H, He J, Lu Z, Liu X - Virol. J. (2010)

Bottom Line: The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification.The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene.The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008.

View Article: PubMed Central - HTML - PubMed

Affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping No,1, Yanchangpu, Lanzhou, Gansu, 730046, China.

ABSTRACT

Background: Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants.

Case presentation: In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene.

Conclusion: The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China.

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Phylogenetic analysis based on deduced amino acid sequence of complete B2L gene. The phylogenetic tree was constructed by the neighbor-joining algorithm using MEGA 4.0, and bootstrap analysis was performed with 1000 trials. All sequences were collected from GenBank. The red spot indicates CHINA ORFV, isolated in this study.
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Figure 3: Phylogenetic analysis based on deduced amino acid sequence of complete B2L gene. The phylogenetic tree was constructed by the neighbor-joining algorithm using MEGA 4.0, and bootstrap analysis was performed with 1000 trials. All sequences were collected from GenBank. The red spot indicates CHINA ORFV, isolated in this study.

Mentions: Using nucleotide sequences of the complete B2L gene stored in GenBank (Table 1), the phylogenetic relationships were explored using the neighbor-joining method and bootstrap analysis (Fig. 3). The results demonstrated that the CHINA/Goat/2009 isolate obtained from this outbreak was closest to the Nantou (DQ934351) isolate obtained from the Tai wan province of China in 2006 and the Hoping (EU935106) isolate, which originated from South Korea in 2008 (Fig. 3). The percent identities and diversities of the deduced amino acid sequence of the B2L gene among the different strains of ORFV were calculated (Table 2) using the MegAlign function of DNASTAR software. The sequence analysis revealed high nucleotide and amino acid identity among the isolates from different countries; they shared 95.3%-99.7% sequence identity at the amino acid level. This is consistent with the fact that the central region of Parapoxviruses is generally conserved.


Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report.

Zhang K, Shang Y, Jin Y, Wang G, Zheng H, He J, Lu Z, Liu X - Virol. J. (2010)

Phylogenetic analysis based on deduced amino acid sequence of complete B2L gene. The phylogenetic tree was constructed by the neighbor-joining algorithm using MEGA 4.0, and bootstrap analysis was performed with 1000 trials. All sequences were collected from GenBank. The red spot indicates CHINA ORFV, isolated in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2877020&req=5

Figure 3: Phylogenetic analysis based on deduced amino acid sequence of complete B2L gene. The phylogenetic tree was constructed by the neighbor-joining algorithm using MEGA 4.0, and bootstrap analysis was performed with 1000 trials. All sequences were collected from GenBank. The red spot indicates CHINA ORFV, isolated in this study.
Mentions: Using nucleotide sequences of the complete B2L gene stored in GenBank (Table 1), the phylogenetic relationships were explored using the neighbor-joining method and bootstrap analysis (Fig. 3). The results demonstrated that the CHINA/Goat/2009 isolate obtained from this outbreak was closest to the Nantou (DQ934351) isolate obtained from the Tai wan province of China in 2006 and the Hoping (EU935106) isolate, which originated from South Korea in 2008 (Fig. 3). The percent identities and diversities of the deduced amino acid sequence of the B2L gene among the different strains of ORFV were calculated (Table 2) using the MegAlign function of DNASTAR software. The sequence analysis revealed high nucleotide and amino acid identity among the isolates from different countries; they shared 95.3%-99.7% sequence identity at the amino acid level. This is consistent with the fact that the central region of Parapoxviruses is generally conserved.

Bottom Line: The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification.The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene.The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008.

View Article: PubMed Central - HTML - PubMed

Affiliation: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping No,1, Yanchangpu, Lanzhou, Gansu, 730046, China.

ABSTRACT

Background: Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants.

Case presentation: In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene.

Conclusion: The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China.

Show MeSH
Related in: MedlinePlus