Limits...
Clonal status and clinicopathological observation of cervical minimal deviation adenocarcinoma.

Gong L, Zhang WD, Liu XY, Han XJ, Yao L, Zhu SJ, Lan M, Li YH, Zhang W - Diagn Pathol (2010)

Bottom Line: The results demonstrated that the glands were positive for CEA, Ki-67, and p53 and negative for estrogen receptor (ER), progesterone receptor (PR), and high-risk human papilloma virus (HPV) DNA.Thus, our findings indicate that MDA is a true neoplasm but is not associated with high-risk HPV.Diagnosis of MDA depends mainly on its clinical manifestations, the pathological feature that MDA glands are located deeper than the lower level of normal endocervical glands, and immunostaining.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tangdu Hospital, the Fourth Military Medical University, Shaanxi Xi'an 710038, China. lyhzhw@fmmu.edu.cn

ABSTRACT

Background: Minimal deviation adenocarcinoma (MDA) of the uterine cervix is defined as an extremely well differentiated variant of cervical adenocarcinoma, with well-formed glands that resemble benign glands but show distinct nuclear anaplasia or evidence of stromal invasion. Thus, MDA is difficult to differentiate from other cervical hyperplastic lesions. Monoclonality is a major characteristic of most tumors, whereas normal tissue and reactive hyperplasia are polyclonal.

Methods: The clinicopathological features and clonality of MDA were investigated using laser microdissection and a clonality assay based on the polymorphism of androgen receptor (AR) and X-chromosomal inactivation mosaicism in female somatic tissues.

Results: The results demonstrated that the glands were positive for CEA, Ki-67, and p53 and negative for estrogen receptor (ER), progesterone receptor (PR), and high-risk human papilloma virus (HPV) DNA. The index of proliferation for Ki-67 was more than 50%. However, the stromal cells were positive for ER, PR, vimentin, and SM-actin. The clonal assay showed that MDA was monoclonal. Thus, our findings indicate that MDA is a true neoplasm but is not associated with high-risk HPV.

Conclusions: Diagnosis of MDA depends mainly on its clinical manifestations, the pathological feature that MDA glands are located deeper than the lower level of normal endocervical glands, and immunostaining.

Show MeSH

Related in: MedlinePlus

A distorted gland before laser microdissection (1A); after laser microdissection (1B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2877003&req=5

Figure 1: A distorted gland before laser microdissection (1A); after laser microdissection (1B).

Mentions: Eight 10-μm tissue sections (1.0 × 1.0 cm2), obtained from representative paraffin blocks, were placed on a UV-absorbing membrane and underwent laser microdissection by LMD6000 (Leica Microsystems Ltd., Wetzlar, Germany). After HE-staining, the slides were mounted on a microstat, and the distorted glands were then dissected by UV laser using the motorized optical-beam scanning mode (Figure 1). The dissected (with the attached specimen) was dropped by gravity into the cap of 0.5-mL microcentrifuge tube filled with 40 μL lysate buffer and 10 μL proteinase K. For each dissected lesion, stromal cells of approximately the same area were isolated and analyzed as a control. The microcentrifuge tubes were then placed in a water bath (48°C) to digest the tissues. After digestion for 12 to 20 h, genomic DNA was extracted and examined by 2% agarose gel electrophoresis, then stored at -20°C.


Clonal status and clinicopathological observation of cervical minimal deviation adenocarcinoma.

Gong L, Zhang WD, Liu XY, Han XJ, Yao L, Zhu SJ, Lan M, Li YH, Zhang W - Diagn Pathol (2010)

A distorted gland before laser microdissection (1A); after laser microdissection (1B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2877003&req=5

Figure 1: A distorted gland before laser microdissection (1A); after laser microdissection (1B).
Mentions: Eight 10-μm tissue sections (1.0 × 1.0 cm2), obtained from representative paraffin blocks, were placed on a UV-absorbing membrane and underwent laser microdissection by LMD6000 (Leica Microsystems Ltd., Wetzlar, Germany). After HE-staining, the slides were mounted on a microstat, and the distorted glands were then dissected by UV laser using the motorized optical-beam scanning mode (Figure 1). The dissected (with the attached specimen) was dropped by gravity into the cap of 0.5-mL microcentrifuge tube filled with 40 μL lysate buffer and 10 μL proteinase K. For each dissected lesion, stromal cells of approximately the same area were isolated and analyzed as a control. The microcentrifuge tubes were then placed in a water bath (48°C) to digest the tissues. After digestion for 12 to 20 h, genomic DNA was extracted and examined by 2% agarose gel electrophoresis, then stored at -20°C.

Bottom Line: The results demonstrated that the glands were positive for CEA, Ki-67, and p53 and negative for estrogen receptor (ER), progesterone receptor (PR), and high-risk human papilloma virus (HPV) DNA.Thus, our findings indicate that MDA is a true neoplasm but is not associated with high-risk HPV.Diagnosis of MDA depends mainly on its clinical manifestations, the pathological feature that MDA glands are located deeper than the lower level of normal endocervical glands, and immunostaining.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tangdu Hospital, the Fourth Military Medical University, Shaanxi Xi'an 710038, China. lyhzhw@fmmu.edu.cn

ABSTRACT

Background: Minimal deviation adenocarcinoma (MDA) of the uterine cervix is defined as an extremely well differentiated variant of cervical adenocarcinoma, with well-formed glands that resemble benign glands but show distinct nuclear anaplasia or evidence of stromal invasion. Thus, MDA is difficult to differentiate from other cervical hyperplastic lesions. Monoclonality is a major characteristic of most tumors, whereas normal tissue and reactive hyperplasia are polyclonal.

Methods: The clinicopathological features and clonality of MDA were investigated using laser microdissection and a clonality assay based on the polymorphism of androgen receptor (AR) and X-chromosomal inactivation mosaicism in female somatic tissues.

Results: The results demonstrated that the glands were positive for CEA, Ki-67, and p53 and negative for estrogen receptor (ER), progesterone receptor (PR), and high-risk human papilloma virus (HPV) DNA. The index of proliferation for Ki-67 was more than 50%. However, the stromal cells were positive for ER, PR, vimentin, and SM-actin. The clonal assay showed that MDA was monoclonal. Thus, our findings indicate that MDA is a true neoplasm but is not associated with high-risk HPV.

Conclusions: Diagnosis of MDA depends mainly on its clinical manifestations, the pathological feature that MDA glands are located deeper than the lower level of normal endocervical glands, and immunostaining.

Show MeSH
Related in: MedlinePlus