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Seven kinds of intermediate filament networks in the cytoplasm of polarized cells: structure and function.

Iwatsuki H, Suda M - Acta Histochem Cytochem (2010)

Bottom Line: However, little information exists on the structure of the IF networks performing these functions.We have clarified the existence of seven kinds of IF networks in the cytoplasm of diverse polarized cells: an apex network just under the terminal web, a peripheral network lying just beneath the cell membrane, a granule-associated network surrounding a mass of secretory granules, a Golgi-associated network surrounding the Golgi apparatus, a radial network locating from the perinuclear region to the specific area of the cell membrane, a juxtanuclear network surrounding the nucleus, and an entire cytoplasmic network.In this review, we describe these seven kinds of IF networks and discuss their biological roles.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Kawasaki Medical School, Matsushima 577, Kurashiki 701-0192, Japan. iwatsuki@med.kawasaki-m.ac.jp

ABSTRACT
Intermediate filaments (IFs) are involved in many important physiological functions, such as the distribution of organelles, signal transduction, cell polarity and gene regulation. However, little information exists on the structure of the IF networks performing these functions. We have clarified the existence of seven kinds of IF networks in the cytoplasm of diverse polarized cells: an apex network just under the terminal web, a peripheral network lying just beneath the cell membrane, a granule-associated network surrounding a mass of secretory granules, a Golgi-associated network surrounding the Golgi apparatus, a radial network locating from the perinuclear region to the specific area of the cell membrane, a juxtanuclear network surrounding the nucleus, and an entire cytoplasmic network. In this review, we describe these seven kinds of IF networks and discuss their biological roles.

No MeSH data available.


Golgi-associated network in absorptive cells of the rabbit duodenum. A: Double immunofluorescence staining of keratin 8 (K8: red) and 14 (K14: green). Both keratins are co-localized as specific ring structures (arrows) in the supranuclear region of the cell. Bar=5 µm. B: Immunoelectron microscopical staining of keratin 14. Keratin 14 is localized around the Golgi apparatus (arrows). Bar=0.5 µm.
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Figure 7: Golgi-associated network in absorptive cells of the rabbit duodenum. A: Double immunofluorescence staining of keratin 8 (K8: red) and 14 (K14: green). Both keratins are co-localized as specific ring structures (arrows) in the supranuclear region of the cell. Bar=5 µm. B: Immunoelectron microscopical staining of keratin 14. Keratin 14 is localized around the Golgi apparatus (arrows). Bar=0.5 µm.

Mentions: As shown in Figure 7A, the Golgi-associated network is observed as a specific ring structure in the supranuclear region. This specific ring structure was confirmed to be a Golgi-associated filament network surrounding the Golgi apparatus by an immunoelectron microscopical study (Fig. 7B). The existence of this network was also confirmed ultrastructurally [66]. Dense bundles of IFs are observed around the Golgi apparatus. The existence of this network is recognized in the various kinds of cells of the rabbit large salivary glands, stomach, small and large intestines, pancreas, trachea, and spinal ganglion [63, 64, 66, 106].


Seven kinds of intermediate filament networks in the cytoplasm of polarized cells: structure and function.

Iwatsuki H, Suda M - Acta Histochem Cytochem (2010)

Golgi-associated network in absorptive cells of the rabbit duodenum. A: Double immunofluorescence staining of keratin 8 (K8: red) and 14 (K14: green). Both keratins are co-localized as specific ring structures (arrows) in the supranuclear region of the cell. Bar=5 µm. B: Immunoelectron microscopical staining of keratin 14. Keratin 14 is localized around the Golgi apparatus (arrows). Bar=0.5 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 7: Golgi-associated network in absorptive cells of the rabbit duodenum. A: Double immunofluorescence staining of keratin 8 (K8: red) and 14 (K14: green). Both keratins are co-localized as specific ring structures (arrows) in the supranuclear region of the cell. Bar=5 µm. B: Immunoelectron microscopical staining of keratin 14. Keratin 14 is localized around the Golgi apparatus (arrows). Bar=0.5 µm.
Mentions: As shown in Figure 7A, the Golgi-associated network is observed as a specific ring structure in the supranuclear region. This specific ring structure was confirmed to be a Golgi-associated filament network surrounding the Golgi apparatus by an immunoelectron microscopical study (Fig. 7B). The existence of this network was also confirmed ultrastructurally [66]. Dense bundles of IFs are observed around the Golgi apparatus. The existence of this network is recognized in the various kinds of cells of the rabbit large salivary glands, stomach, small and large intestines, pancreas, trachea, and spinal ganglion [63, 64, 66, 106].

Bottom Line: However, little information exists on the structure of the IF networks performing these functions.We have clarified the existence of seven kinds of IF networks in the cytoplasm of diverse polarized cells: an apex network just under the terminal web, a peripheral network lying just beneath the cell membrane, a granule-associated network surrounding a mass of secretory granules, a Golgi-associated network surrounding the Golgi apparatus, a radial network locating from the perinuclear region to the specific area of the cell membrane, a juxtanuclear network surrounding the nucleus, and an entire cytoplasmic network.In this review, we describe these seven kinds of IF networks and discuss their biological roles.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Kawasaki Medical School, Matsushima 577, Kurashiki 701-0192, Japan. iwatsuki@med.kawasaki-m.ac.jp

ABSTRACT
Intermediate filaments (IFs) are involved in many important physiological functions, such as the distribution of organelles, signal transduction, cell polarity and gene regulation. However, little information exists on the structure of the IF networks performing these functions. We have clarified the existence of seven kinds of IF networks in the cytoplasm of diverse polarized cells: an apex network just under the terminal web, a peripheral network lying just beneath the cell membrane, a granule-associated network surrounding a mass of secretory granules, a Golgi-associated network surrounding the Golgi apparatus, a radial network locating from the perinuclear region to the specific area of the cell membrane, a juxtanuclear network surrounding the nucleus, and an entire cytoplasmic network. In this review, we describe these seven kinds of IF networks and discuss their biological roles.

No MeSH data available.