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Immunohistochemical analysis of CXCR4 expression in fibrohistiocytic tumors.

Toyozawa S, Yamamoto Y, Ishida Y, Kondo T, Nakamura Y, Furukawa F - Acta Histochem Cytochem (2010)

Bottom Line: These receptors promote tumor growth and metastasis in response to endogenous chemokines.Moreover, MFH had strong immunoreactivity for CXCR4, CCR6 and CCR7.These findings suggest that the assessment of CXCR4 immunoreactivity in fibrohistiocytic tumors is a useful tool for predicting tumor aggressiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Wakayama Medical University, Wakayama, Japan. seikotoy@wakayama-med.ac.jp

ABSTRACT
Functional chemokine receptors are expressed in many malignant tumors. These receptors promote tumor growth and metastasis in response to endogenous chemokines. We analyzed the expression of CXCR4, CCR6 and CCR7 in fibrohistiocytic tumors, including dermatofibrosarcoma protuberance (DFSP), malignant fibrous histiocytoma (MFH), dermatofibroma (DF) using immunohistochemistry. We also investigated the relationship between CXCR4 and CD34, the latter of which is an immunohistochemical marker for DFSP. We observed a higher expression of CXCR4 in DFSP and MFH as compared with DF. Interestingly, a significantly higher expression of CXCR4 was detected in relapsed DFSP than in non-relapsed DFSP, but no significant differences were detected between non-relapsed DFSP and DFSP with CD34 immunostaining. Moreover, MFH had strong immunoreactivity for CXCR4, CCR6 and CCR7. These findings suggest that the assessment of CXCR4 immunoreactivity in fibrohistiocytic tumors is a useful tool for predicting tumor aggressiveness.

No MeSH data available.


Related in: MedlinePlus

CCR6 immunoexpression in fibrohistiocytic tumors (×200). A. Diffuse CCR6 expression was observed in non-relapsed DFSP. B. Diffuse CCR6 expression was observed in relapsed DFSP. C. Diffuse CCR6 expression was observed in MFH. D. No expression of CCR6 was observed in DF.
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Figure 3: CCR6 immunoexpression in fibrohistiocytic tumors (×200). A. Diffuse CCR6 expression was observed in non-relapsed DFSP. B. Diffuse CCR6 expression was observed in relapsed DFSP. C. Diffuse CCR6 expression was observed in MFH. D. No expression of CCR6 was observed in DF.

Mentions: The immunohistochemical staining results for CCR6 are shown in Figure 3. As shown in Figure 4, the average scores for the CCR6 immunostaining were significantly higher in only MFH as compared with DF (p<0.01). However, no significant difference in immunostaining score was observed between non-relapsed DFSP and relapsed DFSP.


Immunohistochemical analysis of CXCR4 expression in fibrohistiocytic tumors.

Toyozawa S, Yamamoto Y, Ishida Y, Kondo T, Nakamura Y, Furukawa F - Acta Histochem Cytochem (2010)

CCR6 immunoexpression in fibrohistiocytic tumors (×200). A. Diffuse CCR6 expression was observed in non-relapsed DFSP. B. Diffuse CCR6 expression was observed in relapsed DFSP. C. Diffuse CCR6 expression was observed in MFH. D. No expression of CCR6 was observed in DF.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 3: CCR6 immunoexpression in fibrohistiocytic tumors (×200). A. Diffuse CCR6 expression was observed in non-relapsed DFSP. B. Diffuse CCR6 expression was observed in relapsed DFSP. C. Diffuse CCR6 expression was observed in MFH. D. No expression of CCR6 was observed in DF.
Mentions: The immunohistochemical staining results for CCR6 are shown in Figure 3. As shown in Figure 4, the average scores for the CCR6 immunostaining were significantly higher in only MFH as compared with DF (p<0.01). However, no significant difference in immunostaining score was observed between non-relapsed DFSP and relapsed DFSP.

Bottom Line: These receptors promote tumor growth and metastasis in response to endogenous chemokines.Moreover, MFH had strong immunoreactivity for CXCR4, CCR6 and CCR7.These findings suggest that the assessment of CXCR4 immunoreactivity in fibrohistiocytic tumors is a useful tool for predicting tumor aggressiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Wakayama Medical University, Wakayama, Japan. seikotoy@wakayama-med.ac.jp

ABSTRACT
Functional chemokine receptors are expressed in many malignant tumors. These receptors promote tumor growth and metastasis in response to endogenous chemokines. We analyzed the expression of CXCR4, CCR6 and CCR7 in fibrohistiocytic tumors, including dermatofibrosarcoma protuberance (DFSP), malignant fibrous histiocytoma (MFH), dermatofibroma (DF) using immunohistochemistry. We also investigated the relationship between CXCR4 and CD34, the latter of which is an immunohistochemical marker for DFSP. We observed a higher expression of CXCR4 in DFSP and MFH as compared with DF. Interestingly, a significantly higher expression of CXCR4 was detected in relapsed DFSP than in non-relapsed DFSP, but no significant differences were detected between non-relapsed DFSP and DFSP with CD34 immunostaining. Moreover, MFH had strong immunoreactivity for CXCR4, CCR6 and CCR7. These findings suggest that the assessment of CXCR4 immunoreactivity in fibrohistiocytic tumors is a useful tool for predicting tumor aggressiveness.

No MeSH data available.


Related in: MedlinePlus