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Immunohistochemical examination on the distribution of cells expressed lymphatic endothelial marker podoplanin and LYVE-1 in the mouse tongue tissue.

Noda Y, Amano I, Hata M, Kojima H, Sawa Y - Acta Histochem Cytochem (2010)

Bottom Line: In the mouse tongue, podoplanin is expressed in nerve sheaths, lingual gland myoepithelial cells, and lymphatic vessels.The immunostaining of factor-VIII on lymphatic vessels was masked by the immunostaining to LYVE-1 or podoplanin because lymphatic vessels express factor-VIII to a far lesser extent than blood vessels.Therefore, except for the salivary glands, the combination of podoplanin and α-SMA, or factor-VIII is useful to identify lymphatic vessels and blood vessels with smooth muscle, or blood capillaries.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Growth & Development, Fukuoka Dental College, 2-15-1 Tamura, Sawara-Ku Fukuoka 814-0193, Japan.

ABSTRACT
The clinical study for lingual disease requires the detailed investigation of the lingual lymphatic network and lymphatic marker-positive cells. Recently, it has been reported that several tissue cells and leukocytes express lymphatic markers, LYVE-1 and podoplanin. This study was aimed to clarify the lingual distribution of cells expressing LYVE-1 and podoplanin. In the mouse tongue, podoplanin is expressed in nerve sheaths, lingual gland myoepithelial cells, and lymphatic vessels. LYVE-1 is expressed in the macrophage marker Mac-1-positive cells as well as lymphatic vessels, while factor-VIII was detected in only blood endothelial cells. α-SMA was detected in vascular smooth muscle and myoepithelial cells. Therefore, identification of lymphatic vessels in lingual glands, the combination of LYVE-1 and factor-VIII, or LYVE-1 and Mac-1 is useful because myoepithelial cells express podoplanin and α-SMA. The immunostaining of factor-VIII on lymphatic vessels was masked by the immunostaining to LYVE-1 or podoplanin because lymphatic vessels express factor-VIII to a far lesser extent than blood vessels. Therefore, except for the salivary glands, the combination of podoplanin and α-SMA, or factor-VIII is useful to identify lymphatic vessels and blood vessels with smooth muscle, or blood capillaries.

No MeSH data available.


Related in: MedlinePlus

Immunostaining for podoplanin and LYVE-1 of mouse apex linguae sections. H-E staining shows the intrinsic lingual muscle and nerve sheaths in the tunica muscularis. Nerve sheaths are stained with only anti-podoplanin antibody in green (arrows), single cells with only anti-LYVE-1 antibody in red (arrowheads), and lymphatic vessels with both anti-podoplanin and anti-LYVE-1 antibodies in yellow (asterisk). Bar=100 µm.
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Figure 1: Immunostaining for podoplanin and LYVE-1 of mouse apex linguae sections. H-E staining shows the intrinsic lingual muscle and nerve sheaths in the tunica muscularis. Nerve sheaths are stained with only anti-podoplanin antibody in green (arrows), single cells with only anti-LYVE-1 antibody in red (arrowheads), and lymphatic vessels with both anti-podoplanin and anti-LYVE-1 antibodies in yellow (asterisk). Bar=100 µm.

Mentions: In the double immunostaining for podoplanin and LYVE-1 of the tunica musclaris of the mouse apex linguae, there were nerve sheaths stained with only anti-podoplanin antibody, single cells with only anti-LYVE-1 antibody, and lymphatic vessels with both anti-podoplanin and anti-LYVE-1 antibodies (Fig. 1). In the double immunostaining for LYVE-1 and Mac-1 of the lamina propria mucosae of the mouse apex linguae, there were lymphatic vessels stained with only anti-LYVE-1 antibody, macrophages with only anti-Mac-1 antibody, and macrophages with both anti-LYVE-1 and anti-Mac-1 antibodies (Fig. 2). In the double immunostaining for podoplanin and α-SMA of the lamina propria mucosae of the mouse radix linguae, lymphatic vessels and blood vessels were stained with only anti-podoplanin antibody and anti-α-SMA antibody, respectively (Fig. 3). It was also observed that in the lingual gland, which is a mixed minor salivary gland composed of mucous and serous acini, the myoepithelial cells surrounding acinar cells were all stained with both anti-podoplanin and anti-α-SMA antibodies (Fig. 3). In the double immunostaining for LYVE-1 and factor-VIII of the mouse sublingual gland which is a mixed major salivary gland mainly composed of mucous acini, lymphatic vessels and blood vessels were stained with only anti-LYVE-1 antibody and anti-factor-VIII antibody, respectively, and there were no myoepithelial, duct and acinar cells, expressing LYVE-1 or factor-VIII (Fig. 4). In the double immunostaining for podoplanin and α-SMA of the tunica muscularis of the mouse radix linguae, lymphatic vessels and blood vessels were stained with only anti-podoplanin antibody and anti-α-SMA antibody, respectively, and there were nerve sheaths stained with only anti-podoplanin antibody (Fig. 5). In the double immunostaining for LYVE-1 and factor-VIII of the lamina propria mucosae of the mouse apex linguae, lymphatic vessels and blood vessels were stained with only anti-podoplanin antibody and factor-VIII antibody, respectively (Fig. 6).


Immunohistochemical examination on the distribution of cells expressed lymphatic endothelial marker podoplanin and LYVE-1 in the mouse tongue tissue.

Noda Y, Amano I, Hata M, Kojima H, Sawa Y - Acta Histochem Cytochem (2010)

Immunostaining for podoplanin and LYVE-1 of mouse apex linguae sections. H-E staining shows the intrinsic lingual muscle and nerve sheaths in the tunica muscularis. Nerve sheaths are stained with only anti-podoplanin antibody in green (arrows), single cells with only anti-LYVE-1 antibody in red (arrowheads), and lymphatic vessels with both anti-podoplanin and anti-LYVE-1 antibodies in yellow (asterisk). Bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875857&req=5

Figure 1: Immunostaining for podoplanin and LYVE-1 of mouse apex linguae sections. H-E staining shows the intrinsic lingual muscle and nerve sheaths in the tunica muscularis. Nerve sheaths are stained with only anti-podoplanin antibody in green (arrows), single cells with only anti-LYVE-1 antibody in red (arrowheads), and lymphatic vessels with both anti-podoplanin and anti-LYVE-1 antibodies in yellow (asterisk). Bar=100 µm.
Mentions: In the double immunostaining for podoplanin and LYVE-1 of the tunica musclaris of the mouse apex linguae, there were nerve sheaths stained with only anti-podoplanin antibody, single cells with only anti-LYVE-1 antibody, and lymphatic vessels with both anti-podoplanin and anti-LYVE-1 antibodies (Fig. 1). In the double immunostaining for LYVE-1 and Mac-1 of the lamina propria mucosae of the mouse apex linguae, there were lymphatic vessels stained with only anti-LYVE-1 antibody, macrophages with only anti-Mac-1 antibody, and macrophages with both anti-LYVE-1 and anti-Mac-1 antibodies (Fig. 2). In the double immunostaining for podoplanin and α-SMA of the lamina propria mucosae of the mouse radix linguae, lymphatic vessels and blood vessels were stained with only anti-podoplanin antibody and anti-α-SMA antibody, respectively (Fig. 3). It was also observed that in the lingual gland, which is a mixed minor salivary gland composed of mucous and serous acini, the myoepithelial cells surrounding acinar cells were all stained with both anti-podoplanin and anti-α-SMA antibodies (Fig. 3). In the double immunostaining for LYVE-1 and factor-VIII of the mouse sublingual gland which is a mixed major salivary gland mainly composed of mucous acini, lymphatic vessels and blood vessels were stained with only anti-LYVE-1 antibody and anti-factor-VIII antibody, respectively, and there were no myoepithelial, duct and acinar cells, expressing LYVE-1 or factor-VIII (Fig. 4). In the double immunostaining for podoplanin and α-SMA of the tunica muscularis of the mouse radix linguae, lymphatic vessels and blood vessels were stained with only anti-podoplanin antibody and anti-α-SMA antibody, respectively, and there were nerve sheaths stained with only anti-podoplanin antibody (Fig. 5). In the double immunostaining for LYVE-1 and factor-VIII of the lamina propria mucosae of the mouse apex linguae, lymphatic vessels and blood vessels were stained with only anti-podoplanin antibody and factor-VIII antibody, respectively (Fig. 6).

Bottom Line: In the mouse tongue, podoplanin is expressed in nerve sheaths, lingual gland myoepithelial cells, and lymphatic vessels.The immunostaining of factor-VIII on lymphatic vessels was masked by the immunostaining to LYVE-1 or podoplanin because lymphatic vessels express factor-VIII to a far lesser extent than blood vessels.Therefore, except for the salivary glands, the combination of podoplanin and α-SMA, or factor-VIII is useful to identify lymphatic vessels and blood vessels with smooth muscle, or blood capillaries.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Growth & Development, Fukuoka Dental College, 2-15-1 Tamura, Sawara-Ku Fukuoka 814-0193, Japan.

ABSTRACT
The clinical study for lingual disease requires the detailed investigation of the lingual lymphatic network and lymphatic marker-positive cells. Recently, it has been reported that several tissue cells and leukocytes express lymphatic markers, LYVE-1 and podoplanin. This study was aimed to clarify the lingual distribution of cells expressing LYVE-1 and podoplanin. In the mouse tongue, podoplanin is expressed in nerve sheaths, lingual gland myoepithelial cells, and lymphatic vessels. LYVE-1 is expressed in the macrophage marker Mac-1-positive cells as well as lymphatic vessels, while factor-VIII was detected in only blood endothelial cells. α-SMA was detected in vascular smooth muscle and myoepithelial cells. Therefore, identification of lymphatic vessels in lingual glands, the combination of LYVE-1 and factor-VIII, or LYVE-1 and Mac-1 is useful because myoepithelial cells express podoplanin and α-SMA. The immunostaining of factor-VIII on lymphatic vessels was masked by the immunostaining to LYVE-1 or podoplanin because lymphatic vessels express factor-VIII to a far lesser extent than blood vessels. Therefore, except for the salivary glands, the combination of podoplanin and α-SMA, or factor-VIII is useful to identify lymphatic vessels and blood vessels with smooth muscle, or blood capillaries.

No MeSH data available.


Related in: MedlinePlus