Limits...
Cholinergic influence on memory stages: A study on scopolamine amnesic mice.

Agrawal R, Tyagi E, Saxena G, Nath C - Indian J Pharmacol (2009)

Bottom Line: The study was planned to determine cholinergic influence on different stages of memory - acquisition, consolidation and recall in scopolamine-induced amnesia (memory impairment) in mice.Results show that acquisition and consolidation are more susceptible to the scopolamine effects than recall.Thus, it may be concluded that cholinergic influence is more on acquisition and consolidation as compared to recall.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Central Drug Research Institute, Lucknow - 226 001, India.

ABSTRACT

Objectives: The study was planned to determine cholinergic influence on different stages of memory - acquisition, consolidation and recall in scopolamine-induced amnesia (memory impairment) in mice.

Materials and methods: To study acquision, consolidation and recall stages of memory, we administered scopolamine (0.75, 1.5 and 3 mg/kg ip) 30 minutes and five minutes prior to first trial acquisition and consolidation and 30 minutes prior to second trial recall of passive avoidance (PA) test, respectively, in separate groups. Tacrine (5 mg/kg po) and rivastigmine (5 mg/kg po) were administered one hour prior to first trial in separate groups which received scopolamine (3 mg/kg ip) 30 minutes and five minutes prior to first trial where as the control group received vehicle only.

Results: In the control group, there was a significant (P < 0.01) increase in transfer latency time (TLT) in the second trial compared to first indicating successful learning. In scopolamine treated groups, administering scopolamine 30 minutes or five minutes prior to first trial did not show any significant (P > 0.05) change in TLT whereas mice treated with scopolamine 30 minutes prior to second trial showed significant (P < 0.01) increase in TLT in second trial as compared to the first. Both tacrine and rivastigmine administration in scopolamine treated mice showed significant (P < 0.05-0.01) increase in TLT in second trial as compared to first trial while the rivastigmine treated group showed greater percentage retention compared to tacrine treated group.

Conclusion: Results show that acquisition and consolidation are more susceptible to the scopolamine effects than recall. Thus, it may be concluded that cholinergic influence is more on acquisition and consolidation as compared to recall.

No MeSH data available.


Related in: MedlinePlus

Effect of tacrine (TAC; 5 mg/kg po) and rivastigmine (RIVA; 5 mg/kg po) on transfer latency time (TLT) in the PA test to observe the effect on scopolamine (SCO; 3 mg/kg ip) induced impaired (a) acquisition, administered 30 minutes prior to first trial and (b) consolidation, administered five minutes prior to first trial. The maximal time of latency was set at 270s (cutoff time). Values are expressed as mean ± SEM; n = five. *P < 0.05, **P < 0.01 significant increase in TLT on second trial as compared to their respective first trial; student's (paired) ‘t’ test
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2875740&req=5

Figure 0002: Effect of tacrine (TAC; 5 mg/kg po) and rivastigmine (RIVA; 5 mg/kg po) on transfer latency time (TLT) in the PA test to observe the effect on scopolamine (SCO; 3 mg/kg ip) induced impaired (a) acquisition, administered 30 minutes prior to first trial and (b) consolidation, administered five minutes prior to first trial. The maximal time of latency was set at 270s (cutoff time). Values are expressed as mean ± SEM; n = five. *P < 0.05, **P < 0.01 significant increase in TLT on second trial as compared to their respective first trial; student's (paired) ‘t’ test

Mentions: There was a significant increase [P < 0.01, df = four] in TLT on second trial as compared to first trial in saline treated (30 minutes prior to first trial) control group indicating that animals had acquired the task, whereas mice treated with scopolamine (3 mg/kg ip), 30 minutes prior to first trial, did not show any significant change [P > 0.05, df = four] in TLT time on second trial as compared to first trial indicating no acquisition. The tacrine (5 mg/kg po) and rivastigmine (5 mg/kg po) administration (one hour prior to first trial) in scopolamine treated group showed significant increase [P < 0.05, df = four; P < 0.01, df = four] in TLT on second trial as compared to first trial. There was no significant difference [F(3, 16) = 2.856, P > 0.05] in TLT in the first trial of different groups [Figure 2a].


Cholinergic influence on memory stages: A study on scopolamine amnesic mice.

Agrawal R, Tyagi E, Saxena G, Nath C - Indian J Pharmacol (2009)

Effect of tacrine (TAC; 5 mg/kg po) and rivastigmine (RIVA; 5 mg/kg po) on transfer latency time (TLT) in the PA test to observe the effect on scopolamine (SCO; 3 mg/kg ip) induced impaired (a) acquisition, administered 30 minutes prior to first trial and (b) consolidation, administered five minutes prior to first trial. The maximal time of latency was set at 270s (cutoff time). Values are expressed as mean ± SEM; n = five. *P < 0.05, **P < 0.01 significant increase in TLT on second trial as compared to their respective first trial; student's (paired) ‘t’ test
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2875740&req=5

Figure 0002: Effect of tacrine (TAC; 5 mg/kg po) and rivastigmine (RIVA; 5 mg/kg po) on transfer latency time (TLT) in the PA test to observe the effect on scopolamine (SCO; 3 mg/kg ip) induced impaired (a) acquisition, administered 30 minutes prior to first trial and (b) consolidation, administered five minutes prior to first trial. The maximal time of latency was set at 270s (cutoff time). Values are expressed as mean ± SEM; n = five. *P < 0.05, **P < 0.01 significant increase in TLT on second trial as compared to their respective first trial; student's (paired) ‘t’ test
Mentions: There was a significant increase [P < 0.01, df = four] in TLT on second trial as compared to first trial in saline treated (30 minutes prior to first trial) control group indicating that animals had acquired the task, whereas mice treated with scopolamine (3 mg/kg ip), 30 minutes prior to first trial, did not show any significant change [P > 0.05, df = four] in TLT time on second trial as compared to first trial indicating no acquisition. The tacrine (5 mg/kg po) and rivastigmine (5 mg/kg po) administration (one hour prior to first trial) in scopolamine treated group showed significant increase [P < 0.05, df = four; P < 0.01, df = four] in TLT on second trial as compared to first trial. There was no significant difference [F(3, 16) = 2.856, P > 0.05] in TLT in the first trial of different groups [Figure 2a].

Bottom Line: The study was planned to determine cholinergic influence on different stages of memory - acquisition, consolidation and recall in scopolamine-induced amnesia (memory impairment) in mice.Results show that acquisition and consolidation are more susceptible to the scopolamine effects than recall.Thus, it may be concluded that cholinergic influence is more on acquisition and consolidation as compared to recall.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Central Drug Research Institute, Lucknow - 226 001, India.

ABSTRACT

Objectives: The study was planned to determine cholinergic influence on different stages of memory - acquisition, consolidation and recall in scopolamine-induced amnesia (memory impairment) in mice.

Materials and methods: To study acquision, consolidation and recall stages of memory, we administered scopolamine (0.75, 1.5 and 3 mg/kg ip) 30 minutes and five minutes prior to first trial acquisition and consolidation and 30 minutes prior to second trial recall of passive avoidance (PA) test, respectively, in separate groups. Tacrine (5 mg/kg po) and rivastigmine (5 mg/kg po) were administered one hour prior to first trial in separate groups which received scopolamine (3 mg/kg ip) 30 minutes and five minutes prior to first trial where as the control group received vehicle only.

Results: In the control group, there was a significant (P < 0.01) increase in transfer latency time (TLT) in the second trial compared to first indicating successful learning. In scopolamine treated groups, administering scopolamine 30 minutes or five minutes prior to first trial did not show any significant (P > 0.05) change in TLT whereas mice treated with scopolamine 30 minutes prior to second trial showed significant (P < 0.01) increase in TLT in second trial as compared to the first. Both tacrine and rivastigmine administration in scopolamine treated mice showed significant (P < 0.05-0.01) increase in TLT in second trial as compared to first trial while the rivastigmine treated group showed greater percentage retention compared to tacrine treated group.

Conclusion: Results show that acquisition and consolidation are more susceptible to the scopolamine effects than recall. Thus, it may be concluded that cholinergic influence is more on acquisition and consolidation as compared to recall.

No MeSH data available.


Related in: MedlinePlus