Limits...
Physicochemical and biological properties of self-assembled antisense/poly(amidoamine) dendrimer nanoparticles: the effect of dendrimer generation and charge ratio.

Nomani A, Haririan I, Rahimnia R, Fouladdel S, Gazori T, Dinarvand R, Omidi Y, Azizi E - Int J Nanomedicine (2010)

Bottom Line: PCS and AFM results revealed that, in contrast to larger molecules of DNA, smaller molecules produce more heterodisperse and large nanoparticles when they are condensed with a cationic dendrimer.For cell uptake, self-assembled nanoparticles were prepared with PAMAM G5 and 5'-FITC labeled antisense and the uptake experiment was carried out in T47D cell culture.This investigation also shows that the cytotoxicity of the nanoparticles was dependent upon the generation and charge ratio of the PAMAM dendrimer, and the antisense concentration had no significant effect on the cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
To gain a deeper understanding of the physicochemical phenomenon of self-assembled nanoparticles of different generations and ratios of poly (amidoamine) dendrimer (PAMAM) dendrimer and a short-stranded DNA (antisense oligonucleotide), multiple methods were used to characterize these nanoparticles including photon correlation spectroscopy (PCS); zeta potential measurement; and atomic force microscopy (AFM). PCS and AFM results revealed that, in contrast to larger molecules of DNA, smaller molecules produce more heterodisperse and large nanoparticles when they are condensed with a cationic dendrimer. AFM images also showed that such nanoparticles were spherical. The stability of the antisense content of the nanoparticles was investigated over different charge ratios using polyacrylamide gel electrophoresis. It was clear from such analyses that much more than charge neutrality point was required to obtain stable nanoparticles. For cell uptake, self-assembled nanoparticles were prepared with PAMAM G5 and 5'-FITC labeled antisense and the uptake experiment was carried out in T47D cell culture. This investigation also shows that the cytotoxicity of the nanoparticles was dependent upon the generation and charge ratio of the PAMAM dendrimer, and the antisense concentration had no significant effect on the cytotoxicity.

Show MeSH
PAGE analysis of PAMAM G5/ANS nanoparticles carried out at various N/P ratios shown in the image.Abbreviations: PAMAM, poly(amidoamine) dendrimer; ANS, antisense oligonucleotide; PAGE, polyacrylamide gel electrophoresis
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2875730&req=5

f3-ijn-5-359: PAGE analysis of PAMAM G5/ANS nanoparticles carried out at various N/P ratios shown in the image.Abbreviations: PAMAM, poly(amidoamine) dendrimer; ANS, antisense oligonucleotide; PAGE, polyacrylamide gel electrophoresis

Mentions: PAGE experiments showed that N/P ratios higher than 2.5 were necessary in order to stabilize and retard the ANS content of PAMAM G5/ANS self-assembled nanoparticles. Figure 3 shows that the lower N/P ratio of 0.5 could also move through the gel at almost the same speed as the naked ANS. But at N/P ratios of 2.5 or higher, almost all of the ANS cargo remained in the wells.


Physicochemical and biological properties of self-assembled antisense/poly(amidoamine) dendrimer nanoparticles: the effect of dendrimer generation and charge ratio.

Nomani A, Haririan I, Rahimnia R, Fouladdel S, Gazori T, Dinarvand R, Omidi Y, Azizi E - Int J Nanomedicine (2010)

PAGE analysis of PAMAM G5/ANS nanoparticles carried out at various N/P ratios shown in the image.Abbreviations: PAMAM, poly(amidoamine) dendrimer; ANS, antisense oligonucleotide; PAGE, polyacrylamide gel electrophoresis
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875730&req=5

f3-ijn-5-359: PAGE analysis of PAMAM G5/ANS nanoparticles carried out at various N/P ratios shown in the image.Abbreviations: PAMAM, poly(amidoamine) dendrimer; ANS, antisense oligonucleotide; PAGE, polyacrylamide gel electrophoresis
Mentions: PAGE experiments showed that N/P ratios higher than 2.5 were necessary in order to stabilize and retard the ANS content of PAMAM G5/ANS self-assembled nanoparticles. Figure 3 shows that the lower N/P ratio of 0.5 could also move through the gel at almost the same speed as the naked ANS. But at N/P ratios of 2.5 or higher, almost all of the ANS cargo remained in the wells.

Bottom Line: PCS and AFM results revealed that, in contrast to larger molecules of DNA, smaller molecules produce more heterodisperse and large nanoparticles when they are condensed with a cationic dendrimer.For cell uptake, self-assembled nanoparticles were prepared with PAMAM G5 and 5'-FITC labeled antisense and the uptake experiment was carried out in T47D cell culture.This investigation also shows that the cytotoxicity of the nanoparticles was dependent upon the generation and charge ratio of the PAMAM dendrimer, and the antisense concentration had no significant effect on the cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
To gain a deeper understanding of the physicochemical phenomenon of self-assembled nanoparticles of different generations and ratios of poly (amidoamine) dendrimer (PAMAM) dendrimer and a short-stranded DNA (antisense oligonucleotide), multiple methods were used to characterize these nanoparticles including photon correlation spectroscopy (PCS); zeta potential measurement; and atomic force microscopy (AFM). PCS and AFM results revealed that, in contrast to larger molecules of DNA, smaller molecules produce more heterodisperse and large nanoparticles when they are condensed with a cationic dendrimer. AFM images also showed that such nanoparticles were spherical. The stability of the antisense content of the nanoparticles was investigated over different charge ratios using polyacrylamide gel electrophoresis. It was clear from such analyses that much more than charge neutrality point was required to obtain stable nanoparticles. For cell uptake, self-assembled nanoparticles were prepared with PAMAM G5 and 5'-FITC labeled antisense and the uptake experiment was carried out in T47D cell culture. This investigation also shows that the cytotoxicity of the nanoparticles was dependent upon the generation and charge ratio of the PAMAM dendrimer, and the antisense concentration had no significant effect on the cytotoxicity.

Show MeSH