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Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium.

Tran PA, Sarin L, Hurt RH, Webster TJ - Int J Nanomedicine (2010)

Bottom Line: Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities.For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment.This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, BrownUniversity, Providence, RI 02912, USA.

ABSTRACT
In the present study, selenium (Se) nanoclusters were grown through heterogeneous nucleation on titanium (Ti) surfaces, a common orthopedic implant material. Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities. For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment. This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

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Increased cancerous osteoblast density after 65 hours of coculturing cancerous and healthy osteoblasts on uncoated Ti. Healthy osteoblast densities showed no significant change on uncoated Ti.Notes: Data = mean ± SEM; N = 3.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
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f7-ijn-5-351: Increased cancerous osteoblast density after 65 hours of coculturing cancerous and healthy osteoblasts on uncoated Ti. Healthy osteoblast densities showed no significant change on uncoated Ti.Notes: Data = mean ± SEM; N = 3.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.

Mentions: Coculture experiments were conducted only for two types of substrates, uTi (control) and High-nSe-Ti (which is the Se-coated substrate that had the highest normal healthy osteoblast density and the lowest cancerous osteoblast density from individual cell experiments). On the uncoated Ti substrates, cancerous osteoblast densities increased with time while healthy osteoblast densities did not change significantly among the different time points (Figure 7).


Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium.

Tran PA, Sarin L, Hurt RH, Webster TJ - Int J Nanomedicine (2010)

Increased cancerous osteoblast density after 65 hours of coculturing cancerous and healthy osteoblasts on uncoated Ti. Healthy osteoblast densities showed no significant change on uncoated Ti.Notes: Data = mean ± SEM; N = 3.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2875729&req=5

f7-ijn-5-351: Increased cancerous osteoblast density after 65 hours of coculturing cancerous and healthy osteoblasts on uncoated Ti. Healthy osteoblast densities showed no significant change on uncoated Ti.Notes: Data = mean ± SEM; N = 3.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
Mentions: Coculture experiments were conducted only for two types of substrates, uTi (control) and High-nSe-Ti (which is the Se-coated substrate that had the highest normal healthy osteoblast density and the lowest cancerous osteoblast density from individual cell experiments). On the uncoated Ti substrates, cancerous osteoblast densities increased with time while healthy osteoblast densities did not change significantly among the different time points (Figure 7).

Bottom Line: Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities.For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment.This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, BrownUniversity, Providence, RI 02912, USA.

ABSTRACT
In the present study, selenium (Se) nanoclusters were grown through heterogeneous nucleation on titanium (Ti) surfaces, a common orthopedic implant material. Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities. For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment. This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

Show MeSH
Related in: MedlinePlus