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Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium.

Tran PA, Sarin L, Hurt RH, Webster TJ - Int J Nanomedicine (2010)

Bottom Line: Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities.For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment.This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, BrownUniversity, Providence, RI 02912, USA.

ABSTRACT
In the present study, selenium (Se) nanoclusters were grown through heterogeneous nucleation on titanium (Ti) surfaces, a common orthopedic implant material. Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities. For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment. This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

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Decreased cancerous osteoblast densities on the Se-coated substrates after three days of culture.Notes: Data = mean ± SEM; N = 3; *P < 0.01 compared to uTi; **P < 0.01 compared to Low-nSe-Ti; ***P < 0.05 compared to Medium-nSe-Ti.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
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f5-ijn-5-351: Decreased cancerous osteoblast densities on the Se-coated substrates after three days of culture.Notes: Data = mean ± SEM; N = 3; *P < 0.01 compared to uTi; **P < 0.01 compared to Low-nSe-Ti; ***P < 0.05 compared to Medium-nSe-Ti.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.

Mentions: In contrast, for cancerous osteoblasts, after three days, cell densities were significantly higher on uTi and Low-nSe-Ti than on High-nSe-Ti (Figures 5 and 6). Specifically, cancerous osteoblast densities on Medium-nSe-Ti and High-nSe-Ti were significantly lower than on Low-nSe-Ti. Cancerous osteoblast densities on High-nSe-Ti wer also significantly lower than on Medium-nSe-Ti (Figures 5 and 6).


Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium.

Tran PA, Sarin L, Hurt RH, Webster TJ - Int J Nanomedicine (2010)

Decreased cancerous osteoblast densities on the Se-coated substrates after three days of culture.Notes: Data = mean ± SEM; N = 3; *P < 0.01 compared to uTi; **P < 0.01 compared to Low-nSe-Ti; ***P < 0.05 compared to Medium-nSe-Ti.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2875729&req=5

f5-ijn-5-351: Decreased cancerous osteoblast densities on the Se-coated substrates after three days of culture.Notes: Data = mean ± SEM; N = 3; *P < 0.01 compared to uTi; **P < 0.01 compared to Low-nSe-Ti; ***P < 0.05 compared to Medium-nSe-Ti.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
Mentions: In contrast, for cancerous osteoblasts, after three days, cell densities were significantly higher on uTi and Low-nSe-Ti than on High-nSe-Ti (Figures 5 and 6). Specifically, cancerous osteoblast densities on Medium-nSe-Ti and High-nSe-Ti were significantly lower than on Low-nSe-Ti. Cancerous osteoblast densities on High-nSe-Ti wer also significantly lower than on Medium-nSe-Ti (Figures 5 and 6).

Bottom Line: Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities.For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment.This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, BrownUniversity, Providence, RI 02912, USA.

ABSTRACT
In the present study, selenium (Se) nanoclusters were grown through heterogeneous nucleation on titanium (Ti) surfaces, a common orthopedic implant material. Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities. For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment. This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

Show MeSH
Related in: MedlinePlus