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Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium.

Tran PA, Sarin L, Hurt RH, Webster TJ - Int J Nanomedicine (2010)

Bottom Line: Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities.For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment.This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, BrownUniversity, Providence, RI 02912, USA.

ABSTRACT
In the present study, selenium (Se) nanoclusters were grown through heterogeneous nucleation on titanium (Ti) surfaces, a common orthopedic implant material. Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities. For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment. This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

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Representative SEM images of uncoated Ti A) and Se-coated Ti with low B) medium C) or high D) doses of Se nanoclusters.Note: Scale bars are 500 nm.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
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f1-ijn-5-351: Representative SEM images of uncoated Ti A) and Se-coated Ti with low B) medium C) or high D) doses of Se nanoclusters.Note: Scale bars are 500 nm.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.

Mentions: SEM images of the uncoated and Se-coated material surfaces demonstrated the presence of Se nanoclusters and an apparent cluster surface density that increased from the low-dose to the medium-dose and high-dose Se cases (Figure 1). The Se nanoclusters were approximately 80 nm in diameter. These Ti substrates will be referred to as uTi, Low-nSe-Ti, Medium-nSe-Ti and High-nSe-Ti for uncoated Ti, and low, medium, and high Se dose-coated Ti substrates, respectively.


Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium.

Tran PA, Sarin L, Hurt RH, Webster TJ - Int J Nanomedicine (2010)

Representative SEM images of uncoated Ti A) and Se-coated Ti with low B) medium C) or high D) doses of Se nanoclusters.Note: Scale bars are 500 nm.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875729&req=5

f1-ijn-5-351: Representative SEM images of uncoated Ti A) and Se-coated Ti with low B) medium C) or high D) doses of Se nanoclusters.Note: Scale bars are 500 nm.Abbreviations: Se, selenium; SEM, standard error of mean; Ti, titanium.
Mentions: SEM images of the uncoated and Se-coated material surfaces demonstrated the presence of Se nanoclusters and an apparent cluster surface density that increased from the low-dose to the medium-dose and high-dose Se cases (Figure 1). The Se nanoclusters were approximately 80 nm in diameter. These Ti substrates will be referred to as uTi, Low-nSe-Ti, Medium-nSe-Ti and High-nSe-Ti for uncoated Ti, and low, medium, and high Se dose-coated Ti substrates, respectively.

Bottom Line: Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities.For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment.This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, BrownUniversity, Providence, RI 02912, USA.

ABSTRACT
In the present study, selenium (Se) nanoclusters were grown through heterogeneous nucleation on titanium (Ti) surfaces, a common orthopedic implant material. Normal healthy osteoblasts (bone-forming cells) and cancerous osteoblasts (osteosarcoma) were cultured on the Se-doped surfaces having three different coating densities. For the first time, it is shown that substrates with Se nanoclusters promote normal osteoblast proliferation and inhibit cancerous osteoblast growth in both separate (mono-culture) and coculture experiment. This study suggests that Se surface nanoclusters can be properly engineered to inhibit bone cancer growth while simultaneously promoting the growth of normal bone tissue.

Show MeSH
Related in: MedlinePlus