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Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring.

Sun TP, Shieh HL, Ching CT, Yao YD, Huang SH, Liu CM, Liu WH, Chen CY - Int J Nanomedicine (2010)

Bottom Line: Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl).The response time of the biosensor was about 25 s.In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou,Taiwan, ROC.

ABSTRACT
This study aims to develop an amperometric glucose biosensor, based on carbon nanotubes material for reverse iontophoresis, fabricated by immobilizing a mixture of glucose oxidase (GOD) and multiwalled carbon nanotubes (MWCNT) epoxy-composite, on a planar screen-printed carbon electrode. MWCNT was employed to ensure proper incorporation into the epoxy mixture and faster electron transfer between the GOD and the transducer. Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl). The response time of the biosensor was about 25 s. In addition, the biosensor could be used in conjunction with reverse iontophoresis technique. In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response. Thus, a glucose biosensor based on carbon nanotube composites and incorporated with reverse iontophoresis function was developed.

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Calibration curves of the biosensors to glucose concentration. The lines are best fit found by linear regression. Sensitivity of the biosensor is indicated by the slope of the linear regression line.Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.
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f4-ijn-5-343: Calibration curves of the biosensors to glucose concentration. The lines are best fit found by linear regression. Sensitivity of the biosensor is indicated by the slope of the linear regression line.Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Mentions: From the data of current-time curves, the magnitude of current response was plotted against the glucose concentration (see Figure 4) and the sensitivity of the biosensor was determined by the slope of linear regression of this current response versus glucose concentration. The current response is directly proportional to the glucose concentration over a wide range of concentration (0–4 mM), where correlation coefficient is greater than 0.997. Since the amount of transdermal glucose extraction by reverse iontophoresis is in the order of μM,28 the range of measurement of glucose biosensor designed in this study can detect the normal concentrations of transdermally extracted glucose with good accuracy.


Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring.

Sun TP, Shieh HL, Ching CT, Yao YD, Huang SH, Liu CM, Liu WH, Chen CY - Int J Nanomedicine (2010)

Calibration curves of the biosensors to glucose concentration. The lines are best fit found by linear regression. Sensitivity of the biosensor is indicated by the slope of the linear regression line.Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875728&req=5

f4-ijn-5-343: Calibration curves of the biosensors to glucose concentration. The lines are best fit found by linear regression. Sensitivity of the biosensor is indicated by the slope of the linear regression line.Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.
Mentions: From the data of current-time curves, the magnitude of current response was plotted against the glucose concentration (see Figure 4) and the sensitivity of the biosensor was determined by the slope of linear regression of this current response versus glucose concentration. The current response is directly proportional to the glucose concentration over a wide range of concentration (0–4 mM), where correlation coefficient is greater than 0.997. Since the amount of transdermal glucose extraction by reverse iontophoresis is in the order of μM,28 the range of measurement of glucose biosensor designed in this study can detect the normal concentrations of transdermally extracted glucose with good accuracy.

Bottom Line: Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl).The response time of the biosensor was about 25 s.In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou,Taiwan, ROC.

ABSTRACT
This study aims to develop an amperometric glucose biosensor, based on carbon nanotubes material for reverse iontophoresis, fabricated by immobilizing a mixture of glucose oxidase (GOD) and multiwalled carbon nanotubes (MWCNT) epoxy-composite, on a planar screen-printed carbon electrode. MWCNT was employed to ensure proper incorporation into the epoxy mixture and faster electron transfer between the GOD and the transducer. Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl). The response time of the biosensor was about 25 s. In addition, the biosensor could be used in conjunction with reverse iontophoresis technique. In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response. Thus, a glucose biosensor based on carbon nanotube composites and incorporated with reverse iontophoresis function was developed.

Show MeSH
Related in: MedlinePlus