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Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring.

Sun TP, Shieh HL, Ching CT, Yao YD, Huang SH, Liu CM, Liu WH, Chen CY - Int J Nanomedicine (2010)

Bottom Line: Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl).The response time of the biosensor was about 25 s.In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou,Taiwan, ROC.

ABSTRACT
This study aims to develop an amperometric glucose biosensor, based on carbon nanotubes material for reverse iontophoresis, fabricated by immobilizing a mixture of glucose oxidase (GOD) and multiwalled carbon nanotubes (MWCNT) epoxy-composite, on a planar screen-printed carbon electrode. MWCNT was employed to ensure proper incorporation into the epoxy mixture and faster electron transfer between the GOD and the transducer. Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl). The response time of the biosensor was about 25 s. In addition, the biosensor could be used in conjunction with reverse iontophoresis technique. In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response. Thus, a glucose biosensor based on carbon nanotube composites and incorporated with reverse iontophoresis function was developed.

Show MeSH
Current-time curve obtained by successive addition of 4 mM glucose solution to the biosensor. Data is obtained from biosensor with the combination of MWCNT (18.0% w/w) and GOD (2.0% w/w).Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.
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f3-ijn-5-343: Current-time curve obtained by successive addition of 4 mM glucose solution to the biosensor. Data is obtained from biosensor with the combination of MWCNT (18.0% w/w) and GOD (2.0% w/w).Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.

Mentions: Figure 3 showed a current-time curve, where steps correspond to current response of the biosensor (MWCNT [18.0% w/w] and GOD [2.0% w/w] mixture) at the applied potential of +500 mV, for successive additions of 4 mM glucose in 0.1 M phosphate buffer solution (pH 7.0). Immediately after the addition of glucose solution, the reductive current increased and reached 95% of the steady state current at about 25 seconds. The response time of our biosensor is comparable to that reported by Antiochia and Gorton.44


Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring.

Sun TP, Shieh HL, Ching CT, Yao YD, Huang SH, Liu CM, Liu WH, Chen CY - Int J Nanomedicine (2010)

Current-time curve obtained by successive addition of 4 mM glucose solution to the biosensor. Data is obtained from biosensor with the combination of MWCNT (18.0% w/w) and GOD (2.0% w/w).Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875728&req=5

f3-ijn-5-343: Current-time curve obtained by successive addition of 4 mM glucose solution to the biosensor. Data is obtained from biosensor with the combination of MWCNT (18.0% w/w) and GOD (2.0% w/w).Abbreviations: GOD, glucose oxidase; MWCNT, multiwalled carbon nanotubes.
Mentions: Figure 3 showed a current-time curve, where steps correspond to current response of the biosensor (MWCNT [18.0% w/w] and GOD [2.0% w/w] mixture) at the applied potential of +500 mV, for successive additions of 4 mM glucose in 0.1 M phosphate buffer solution (pH 7.0). Immediately after the addition of glucose solution, the reductive current increased and reached 95% of the steady state current at about 25 seconds. The response time of our biosensor is comparable to that reported by Antiochia and Gorton.44

Bottom Line: Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl).The response time of the biosensor was about 25 s.In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou,Taiwan, ROC.

ABSTRACT
This study aims to develop an amperometric glucose biosensor, based on carbon nanotubes material for reverse iontophoresis, fabricated by immobilizing a mixture of glucose oxidase (GOD) and multiwalled carbon nanotubes (MWCNT) epoxy-composite, on a planar screen-printed carbon electrode. MWCNT was employed to ensure proper incorporation into the epoxy mixture and faster electron transfer between the GOD and the transducer. Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl). The response time of the biosensor was about 25 s. In addition, the biosensor could be used in conjunction with reverse iontophoresis technique. In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response. Thus, a glucose biosensor based on carbon nanotube composites and incorporated with reverse iontophoresis function was developed.

Show MeSH