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Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring.

Sun TP, Shieh HL, Ching CT, Yao YD, Huang SH, Liu CM, Liu WH, Chen CY - Int J Nanomedicine (2010)

Bottom Line: Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl).The response time of the biosensor was about 25 s.In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou,Taiwan, ROC.

ABSTRACT
This study aims to develop an amperometric glucose biosensor, based on carbon nanotubes material for reverse iontophoresis, fabricated by immobilizing a mixture of glucose oxidase (GOD) and multiwalled carbon nanotubes (MWCNT) epoxy-composite, on a planar screen-printed carbon electrode. MWCNT was employed to ensure proper incorporation into the epoxy mixture and faster electron transfer between the GOD and the transducer. Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl). The response time of the biosensor was about 25 s. In addition, the biosensor could be used in conjunction with reverse iontophoresis technique. In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response. Thus, a glucose biosensor based on carbon nanotube composites and incorporated with reverse iontophoresis function was developed.

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Construction steps for the planar configuration of the screen-printed transducer. a) (PETE) support material; b) conducting silver basal track; c) insulation layer; d) Ag/AgCl pads, the iontophoresis electrode (the central circular one) for reverse iontophoresis and the reference electrode; e) graphite pads, the working and counter electrodes.
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f1-ijn-5-343: Construction steps for the planar configuration of the screen-printed transducer. a) (PETE) support material; b) conducting silver basal track; c) insulation layer; d) Ag/AgCl pads, the iontophoresis electrode (the central circular one) for reverse iontophoresis and the reference electrode; e) graphite pads, the working and counter electrodes.

Mentions: The construction steps of a planar three-electrode transducer were shown schematically in Figure 1, according to the procedure described earlier.32 The transducer was then used for glucose biosensor construction.


Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring.

Sun TP, Shieh HL, Ching CT, Yao YD, Huang SH, Liu CM, Liu WH, Chen CY - Int J Nanomedicine (2010)

Construction steps for the planar configuration of the screen-printed transducer. a) (PETE) support material; b) conducting silver basal track; c) insulation layer; d) Ag/AgCl pads, the iontophoresis electrode (the central circular one) for reverse iontophoresis and the reference electrode; e) graphite pads, the working and counter electrodes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875728&req=5

f1-ijn-5-343: Construction steps for the planar configuration of the screen-printed transducer. a) (PETE) support material; b) conducting silver basal track; c) insulation layer; d) Ag/AgCl pads, the iontophoresis electrode (the central circular one) for reverse iontophoresis and the reference electrode; e) graphite pads, the working and counter electrodes.
Mentions: The construction steps of a planar three-electrode transducer were shown schematically in Figure 1, according to the procedure described earlier.32 The transducer was then used for glucose biosensor construction.

Bottom Line: Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl).The response time of the biosensor was about 25 s.In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou,Taiwan, ROC.

ABSTRACT
This study aims to develop an amperometric glucose biosensor, based on carbon nanotubes material for reverse iontophoresis, fabricated by immobilizing a mixture of glucose oxidase (GOD) and multiwalled carbon nanotubes (MWCNT) epoxy-composite, on a planar screen-printed carbon electrode. MWCNT was employed to ensure proper incorporation into the epoxy mixture and faster electron transfer between the GOD and the transducer. Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl). The response time of the biosensor was about 25 s. In addition, the biosensor could be used in conjunction with reverse iontophoresis technique. In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response. Thus, a glucose biosensor based on carbon nanotube composites and incorporated with reverse iontophoresis function was developed.

Show MeSH
Related in: MedlinePlus