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Antibacterial titanium plate deposited by silver nanoparticles exhibits cell compatibility.

Liao J, Anchun M, Zhu Z, Quan Y - Int J Nanomedicine (2010)

Bottom Line: While antibacterial activity is important, biomaterial surfaces should be modified to achieve excellent cell compatibility as well.The results demonstrate that the Ti-nAg do not show any cytotoxicity to the human gingival fibroblasts.Our data indicate that Ti-nAg is a novel material with both good antibacterial properties and uncompromised cytocompatibility, which can be used as an implanted biomaterial.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, PR China.

ABSTRACT
Microbial colonization and biofilm formation on the surface of implant devices may cause peri-implantitis and lead to bone loss. The aim of this study was to develop a novel antibacterial titanium implant surface and to test its biological performance. In a previous study, we demonstrated that titanium plates deposited by nanosilver acquired antibacterial activity to Staphylococcus aureus and Escherichia coli. While antibacterial activity is important, biomaterial surfaces should be modified to achieve excellent cell compatibility as well. In the present study, using the MTT assay, fluorescence microscopy, and scanning electron microscopy, we assessed cell viability, cytoskeletal architecture and cell attachment, respectively, on our silver nanoparticle-modified titanium (Ti-nAg) plate. The results demonstrate that the Ti-nAg do not show any cytotoxicity to the human gingival fibroblasts. Our data indicate that Ti-nAg is a novel material with both good antibacterial properties and uncompromised cytocompatibility, which can be used as an implanted biomaterial.

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Scanning electron photomicrographs of the Ti-nAg (A) and Ti-polished (B) surfaces after incubation of Aa (magnification: 5000×; working distance: 8000 μm). The Aa bacteria exhibited a rod shape and reduced bacterial attachment to the Ti-nAg surface, relative to the Ti-polished surface.
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f2-ijn-5-337: Scanning electron photomicrographs of the Ti-nAg (A) and Ti-polished (B) surfaces after incubation of Aa (magnification: 5000×; working distance: 8000 μm). The Aa bacteria exhibited a rod shape and reduced bacterial attachment to the Ti-nAg surface, relative to the Ti-polished surface.

Mentions: Following 24 hours incubation, 94.79% Aa and >93% Pg of bacteria in suspension were inhibited on the Ti-nAg surface (figure 1). The SEM of Ti-nAg and Ti-polished specimens incubated with Pg or Aa are shown in figures 2 and 3, respectively. The adhesion of both Pg and Aa was markedly decreased on the Ti-nAg surface, compared to the Ti-polished surface. The morphology of the Aa adhered to the surface showed an aggregated appearance, while the Pg was dispersed.


Antibacterial titanium plate deposited by silver nanoparticles exhibits cell compatibility.

Liao J, Anchun M, Zhu Z, Quan Y - Int J Nanomedicine (2010)

Scanning electron photomicrographs of the Ti-nAg (A) and Ti-polished (B) surfaces after incubation of Aa (magnification: 5000×; working distance: 8000 μm). The Aa bacteria exhibited a rod shape and reduced bacterial attachment to the Ti-nAg surface, relative to the Ti-polished surface.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875727&req=5

f2-ijn-5-337: Scanning electron photomicrographs of the Ti-nAg (A) and Ti-polished (B) surfaces after incubation of Aa (magnification: 5000×; working distance: 8000 μm). The Aa bacteria exhibited a rod shape and reduced bacterial attachment to the Ti-nAg surface, relative to the Ti-polished surface.
Mentions: Following 24 hours incubation, 94.79% Aa and >93% Pg of bacteria in suspension were inhibited on the Ti-nAg surface (figure 1). The SEM of Ti-nAg and Ti-polished specimens incubated with Pg or Aa are shown in figures 2 and 3, respectively. The adhesion of both Pg and Aa was markedly decreased on the Ti-nAg surface, compared to the Ti-polished surface. The morphology of the Aa adhered to the surface showed an aggregated appearance, while the Pg was dispersed.

Bottom Line: While antibacterial activity is important, biomaterial surfaces should be modified to achieve excellent cell compatibility as well.The results demonstrate that the Ti-nAg do not show any cytotoxicity to the human gingival fibroblasts.Our data indicate that Ti-nAg is a novel material with both good antibacterial properties and uncompromised cytocompatibility, which can be used as an implanted biomaterial.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, PR China.

ABSTRACT
Microbial colonization and biofilm formation on the surface of implant devices may cause peri-implantitis and lead to bone loss. The aim of this study was to develop a novel antibacterial titanium implant surface and to test its biological performance. In a previous study, we demonstrated that titanium plates deposited by nanosilver acquired antibacterial activity to Staphylococcus aureus and Escherichia coli. While antibacterial activity is important, biomaterial surfaces should be modified to achieve excellent cell compatibility as well. In the present study, using the MTT assay, fluorescence microscopy, and scanning electron microscopy, we assessed cell viability, cytoskeletal architecture and cell attachment, respectively, on our silver nanoparticle-modified titanium (Ti-nAg) plate. The results demonstrate that the Ti-nAg do not show any cytotoxicity to the human gingival fibroblasts. Our data indicate that Ti-nAg is a novel material with both good antibacterial properties and uncompromised cytocompatibility, which can be used as an implanted biomaterial.

Show MeSH
Related in: MedlinePlus