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Tissue engineering scaffold material of porous nanohydroxyapatite/polyamide 66.

Xu Q, Lu H, Zhang J, Lu G, Deng Z, Mo A - Int J Nanomedicine (2010)

Bottom Line: The samples were harvested at 2, 4, 12 and 26 weeks respectively, and subjected to histological analysis.At 2 weeks, the experiment showed that osteogenesis was detected in porous n-HA/PA66 composite and the density of new bone formation was similar to the surrounding host bone at 12 weeks.In conclusion, porous n-HA/PA66 scaffold material could be a good candidate as a bone substitute material used in clinics due to its excellent histocompatibility, osteoconductivity and osteoinductivity.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, PR China.

ABSTRACT
The aim of the study was to investigate a porous nanohydroxyapatite/polyamide 66 (n-HA/PA66) scaffold material that was implanted into muscle and tibiae of 16 New Zealand white rabbits to evaluate the biocompatibility and osteogenesis and osteoinductivity of the materials in vivo. The samples were harvested at 2, 4, 12 and 26 weeks respectively, and subjected to histological analysis. At 2 weeks, the experiment showed that osteogenesis was detected in porous n-HA/PA66 composite and the density of new bone formation was similar to the surrounding host bone at 12 weeks. The study indicated that three-dimensional pore structures could facilitate cell adhesion, differentiation and proliferation, and help with fibrovascular and nerve colonization. In conclusion, porous n-HA/PA66 scaffold material could be a good candidate as a bone substitute material used in clinics due to its excellent histocompatibility, osteoconductivity and osteoinductivity.

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Related in: MedlinePlus

Schematic diagram of material shape and the site of implantation. A) material shape B) the site of intramuscular implantation on the both sides of the spine. C) the site of endosseous implantation in the tibia.
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f1-ijn-5-331: Schematic diagram of material shape and the site of implantation. A) material shape B) the site of intramuscular implantation on the both sides of the spine. C) the site of endosseous implantation in the tibia.

Mentions: Sixteen New Zealand white rabbits (average weight between 2.0 kg and 2.5 kg) were obtained from State Key Laboratory, Chengdu, China, and were divided by random treatment into four groups of four each. The rabbits were anesthetized by intravenous injection of 3% sodium pentobarbital (1 ml/kg) and operated on under sterile conditions. As seen in Figure 1, middle skin incision was performed on the middle of the back, and two discs of porous n-HA/PA66 samples (5 mm in diameter and 10 mm in length) were inserted into the muscular pocket and sutured wounds. At the same time, the samples (2 mm in diameter and 4 mm in length) were implanted into the bone defect which matched the sample size in the tibiae. The rabbits were sacrificed at 2, 4, 12, and 26 weeks respectively. The specimens were submitted to histological assessment.


Tissue engineering scaffold material of porous nanohydroxyapatite/polyamide 66.

Xu Q, Lu H, Zhang J, Lu G, Deng Z, Mo A - Int J Nanomedicine (2010)

Schematic diagram of material shape and the site of implantation. A) material shape B) the site of intramuscular implantation on the both sides of the spine. C) the site of endosseous implantation in the tibia.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875726&req=5

f1-ijn-5-331: Schematic diagram of material shape and the site of implantation. A) material shape B) the site of intramuscular implantation on the both sides of the spine. C) the site of endosseous implantation in the tibia.
Mentions: Sixteen New Zealand white rabbits (average weight between 2.0 kg and 2.5 kg) were obtained from State Key Laboratory, Chengdu, China, and were divided by random treatment into four groups of four each. The rabbits were anesthetized by intravenous injection of 3% sodium pentobarbital (1 ml/kg) and operated on under sterile conditions. As seen in Figure 1, middle skin incision was performed on the middle of the back, and two discs of porous n-HA/PA66 samples (5 mm in diameter and 10 mm in length) were inserted into the muscular pocket and sutured wounds. At the same time, the samples (2 mm in diameter and 4 mm in length) were implanted into the bone defect which matched the sample size in the tibiae. The rabbits were sacrificed at 2, 4, 12, and 26 weeks respectively. The specimens were submitted to histological assessment.

Bottom Line: The samples were harvested at 2, 4, 12 and 26 weeks respectively, and subjected to histological analysis.At 2 weeks, the experiment showed that osteogenesis was detected in porous n-HA/PA66 composite and the density of new bone formation was similar to the surrounding host bone at 12 weeks.In conclusion, porous n-HA/PA66 scaffold material could be a good candidate as a bone substitute material used in clinics due to its excellent histocompatibility, osteoconductivity and osteoinductivity.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, PR China.

ABSTRACT
The aim of the study was to investigate a porous nanohydroxyapatite/polyamide 66 (n-HA/PA66) scaffold material that was implanted into muscle and tibiae of 16 New Zealand white rabbits to evaluate the biocompatibility and osteogenesis and osteoinductivity of the materials in vivo. The samples were harvested at 2, 4, 12 and 26 weeks respectively, and subjected to histological analysis. At 2 weeks, the experiment showed that osteogenesis was detected in porous n-HA/PA66 composite and the density of new bone formation was similar to the surrounding host bone at 12 weeks. The study indicated that three-dimensional pore structures could facilitate cell adhesion, differentiation and proliferation, and help with fibrovascular and nerve colonization. In conclusion, porous n-HA/PA66 scaffold material could be a good candidate as a bone substitute material used in clinics due to its excellent histocompatibility, osteoconductivity and osteoinductivity.

Show MeSH
Related in: MedlinePlus