Limits...
Gold nanoparticle-choline complexes can block nicotinic acetylcholine receptors.

Chin C, Kim IK, Lim DY, Kim KS, Lee HA, Kim EJ - Int J Nanomedicine (2010)

Bottom Line: Negatively charged gold nanoparticles (1.4 nm) block ion pores by binding to the sulfur group of the cysteine loop of nicotinic acetylcholine receptors (nAChRs), and currents evoked by acetylcholine (Ach) can break these bonds.An in vivo study showed muscle relaxation in rats after injection of the complex.These results will foster the application of gold nanoparticles as a direct ion-channel blocker.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Fatima Hospital, Daegu, Korea.

ABSTRACT
We identified a novel class of direct ion-channel blockers of ligand-gated ion channels called the gold nanoparticle-choline complex. Negatively charged gold nanoparticles (1.4 nm) block ion pores by binding to the sulfur group of the cysteine loop of nicotinic acetylcholine receptors (nAChRs), and currents evoked by acetylcholine (Ach) can break these bonds. The current evoked by ACh in nAChRs was blocked directly in ion pores by the gold nanoparticle- choline complex. In adrenal-gland perfusion studies, the complex also blocked nAChRs by diminishing catecholamine release by about 75%. An in vivo study showed muscle relaxation in rats after injection of the complex. These results will foster the application of gold nanoparticles as a direct ion-channel blocker.

Show MeSH

Related in: MedlinePlus

Reversal achieved in catecholamine secretion.Abberviation: DMMPP, dimethyl methyl phosphonate.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2875724&req=5

f3-ijn-5-315: Reversal achieved in catecholamine secretion.Abberviation: DMMPP, dimethyl methyl phosphonate.

Mentions: The secretagogues were administered at 15-min intervals. Gold nanoparticle–choline complexes were detected for 60 min after the establishment of control release. When the cells were treated with the 1 mM choline and 15 nM nanoparticle complex, we confirmed that the CA secretion evoked by ACh and stimulation of the selective neuronal-type AChR agonist, DMPP, were reduced by about 75% after 60 min.8 When ACh was readministered after about 1.5 hr, we confirmed that reversal was achieved (Figure 3).


Gold nanoparticle-choline complexes can block nicotinic acetylcholine receptors.

Chin C, Kim IK, Lim DY, Kim KS, Lee HA, Kim EJ - Int J Nanomedicine (2010)

Reversal achieved in catecholamine secretion.Abberviation: DMMPP, dimethyl methyl phosphonate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2875724&req=5

f3-ijn-5-315: Reversal achieved in catecholamine secretion.Abberviation: DMMPP, dimethyl methyl phosphonate.
Mentions: The secretagogues were administered at 15-min intervals. Gold nanoparticle–choline complexes were detected for 60 min after the establishment of control release. When the cells were treated with the 1 mM choline and 15 nM nanoparticle complex, we confirmed that the CA secretion evoked by ACh and stimulation of the selective neuronal-type AChR agonist, DMPP, were reduced by about 75% after 60 min.8 When ACh was readministered after about 1.5 hr, we confirmed that reversal was achieved (Figure 3).

Bottom Line: Negatively charged gold nanoparticles (1.4 nm) block ion pores by binding to the sulfur group of the cysteine loop of nicotinic acetylcholine receptors (nAChRs), and currents evoked by acetylcholine (Ach) can break these bonds.An in vivo study showed muscle relaxation in rats after injection of the complex.These results will foster the application of gold nanoparticles as a direct ion-channel blocker.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Fatima Hospital, Daegu, Korea.

ABSTRACT
We identified a novel class of direct ion-channel blockers of ligand-gated ion channels called the gold nanoparticle-choline complex. Negatively charged gold nanoparticles (1.4 nm) block ion pores by binding to the sulfur group of the cysteine loop of nicotinic acetylcholine receptors (nAChRs), and currents evoked by acetylcholine (Ach) can break these bonds. The current evoked by ACh in nAChRs was blocked directly in ion pores by the gold nanoparticle- choline complex. In adrenal-gland perfusion studies, the complex also blocked nAChRs by diminishing catecholamine release by about 75%. An in vivo study showed muscle relaxation in rats after injection of the complex. These results will foster the application of gold nanoparticles as a direct ion-channel blocker.

Show MeSH
Related in: MedlinePlus