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The relationship between CD4+CD25+CD127- regulatory T cells and inflammatory response and outcome during shock states.

Hein F, Massin F, Cravoisy-Popovic A, Barraud D, Levy B, Bollaert PE, Gibot S - Crit Care (2010)

Bottom Line: A similar pattern of Tregs kinetics was found in infected and non infected patients.No relation between Tregs and cytokine concentration was found.In septic mice, although there was a rapid increase in Treg cells subset among splenocytes, antibody-induced depletion of Tregs before the onset of sepsis did not alter survival.

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Affiliation: Service de réanimation médicale, Hôpital Central, CHU de Nancy, 29 Bld du Mal de Lattre de Tassigny, Nancy, 54000, France. f.hein@chu-nancy.fr

ABSTRACT

Introduction: Although regulatory T lymphocytes (Tregs) have a pivotal role in preventing autoimmune diseases and limiting chronic inflammatory conditions, they may also block beneficial immune responses by preventing sterilizing immunity to certain pathogens.

Methods: To determine whether naturally occurring Treg cells have a role in inflammatory response and outcome during shock state we conducted an observational study in two adult ICUs from a university hospital. Within 12 hours of admission, peripheral whole blood was collected for the measurement of cytokines and determination of lymphocyte count. Sampling was repeated at day three, five and seven. Furthermore, an experimental septic shock was induced in adult Balb/c mice through caecal ligation and puncture.

Results: Forty-three patients suffering from shock (26 septic, 17 non septic), and 7 healthy volunteers were included. The percentage of Tregs increased as early as 3 days after the onset of shock, while their absolute number remained lower than in healthy volunteers. A similar pattern of Tregs kinetics was found in infected and non infected patients. Though there was an inverse correlation between severity scores and Tregs percentage, the time course of Tregs was similar between survivors and non survivors. No relation between Tregs and cytokine concentration was found. In septic mice, although there was a rapid increase in Treg cells subset among splenocytes, antibody-induced depletion of Tregs before the onset of sepsis did not alter survival.

Conclusions: These data argue against a determinant role of Tregs in inflammatory response and outcome during shock states.

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Related in: MedlinePlus

TNF-α plasma concentration according to the regulatory T lymphocytes status in septic mice. Antibody-mediated depletion of CD25+ cells does not influence TNF-α plasma concentration during caecal ligation and puncture (n = 6 per group).
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Figure 7: TNF-α plasma concentration according to the regulatory T lymphocytes status in septic mice. Antibody-mediated depletion of CD25+ cells does not influence TNF-α plasma concentration during caecal ligation and puncture (n = 6 per group).

Mentions: Animal survival was not affected by the CD25+ cells status with 40% of CD25-depleted mice surviving as compared with 31% for the non-depleted group (P = 0.62; Figure 7).


The relationship between CD4+CD25+CD127- regulatory T cells and inflammatory response and outcome during shock states.

Hein F, Massin F, Cravoisy-Popovic A, Barraud D, Levy B, Bollaert PE, Gibot S - Crit Care (2010)

TNF-α plasma concentration according to the regulatory T lymphocytes status in septic mice. Antibody-mediated depletion of CD25+ cells does not influence TNF-α plasma concentration during caecal ligation and puncture (n = 6 per group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2875534&req=5

Figure 7: TNF-α plasma concentration according to the regulatory T lymphocytes status in septic mice. Antibody-mediated depletion of CD25+ cells does not influence TNF-α plasma concentration during caecal ligation and puncture (n = 6 per group).
Mentions: Animal survival was not affected by the CD25+ cells status with 40% of CD25-depleted mice surviving as compared with 31% for the non-depleted group (P = 0.62; Figure 7).

Bottom Line: A similar pattern of Tregs kinetics was found in infected and non infected patients.No relation between Tregs and cytokine concentration was found.In septic mice, although there was a rapid increase in Treg cells subset among splenocytes, antibody-induced depletion of Tregs before the onset of sepsis did not alter survival.

View Article: PubMed Central - HTML - PubMed

Affiliation: Service de réanimation médicale, Hôpital Central, CHU de Nancy, 29 Bld du Mal de Lattre de Tassigny, Nancy, 54000, France. f.hein@chu-nancy.fr

ABSTRACT

Introduction: Although regulatory T lymphocytes (Tregs) have a pivotal role in preventing autoimmune diseases and limiting chronic inflammatory conditions, they may also block beneficial immune responses by preventing sterilizing immunity to certain pathogens.

Methods: To determine whether naturally occurring Treg cells have a role in inflammatory response and outcome during shock state we conducted an observational study in two adult ICUs from a university hospital. Within 12 hours of admission, peripheral whole blood was collected for the measurement of cytokines and determination of lymphocyte count. Sampling was repeated at day three, five and seven. Furthermore, an experimental septic shock was induced in adult Balb/c mice through caecal ligation and puncture.

Results: Forty-three patients suffering from shock (26 septic, 17 non septic), and 7 healthy volunteers were included. The percentage of Tregs increased as early as 3 days after the onset of shock, while their absolute number remained lower than in healthy volunteers. A similar pattern of Tregs kinetics was found in infected and non infected patients. Though there was an inverse correlation between severity scores and Tregs percentage, the time course of Tregs was similar between survivors and non survivors. No relation between Tregs and cytokine concentration was found. In septic mice, although there was a rapid increase in Treg cells subset among splenocytes, antibody-induced depletion of Tregs before the onset of sepsis did not alter survival.

Conclusions: These data argue against a determinant role of Tregs in inflammatory response and outcome during shock states.

Show MeSH
Related in: MedlinePlus