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Association between regulatory T cell activity and sepsis and outcome of severely burned patients: a prospective, observational study.

Huang LF, Yao YM, Dong N, Yu Y, He LX, Sheng ZY - Crit Care (2010)

Bottom Line: They were also higher in septic patients than those without sepsis.Among septic patients, the expressions of Tregs phenotypes and the levels of cytokines were markedly lower in the survival group than those in patients with fatal outcome.Severe burn injury per se could lead to the changes in Tregs activities.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, Burns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, 51 Fu-cheng Road, Haidian District, Beijing 100048, PR China. burnshlf@sina.com

ABSTRACT

Introduction: To investigate the significance of changes in regulatory T cells (Tregs) activity and its relationship with sepsis, as well as outcome of patients with major burns.

Methods: The periphery blood samples of 106 patients were collected on post-burn days 1, 3, 7, 14, and 21. Tregs were isolated and their phenotypes (cytotoxic T-lymphocyte-associated antigen 4 and forkhead/winged helix transcription factor p3) were analyzed by flow cytometry, and the contents of cytokines (interleukin-10 and transforming growth factor-beta1) released into supernatants by Tregs were also determined by enzyme-linked immunosorbent assay kits. Gene expressions of cytokines were assessed by real-time quantitative polymerase chain reaction.

Results: Expressions of Tregs phenotypes and gene/protein expression of cytokines were all elevated after burn, and there were obvious differences among patients with various burn sizes. They were also higher in septic patients than those without sepsis. Among septic patients, the expressions of Tregs phenotypes and the levels of cytokines were markedly lower in the survival group than those in patients with fatal outcome.

Conclusions: Severe burn injury per se could lead to the changes in Tregs activities. Elevated levels of cytokines produced by Tregs and activation markers on Tregs surface might play an important role in the pathogenesis of sepsis and mortality in burned patients.

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ELISA analysis of IL-10 and TGF-β1 levels in Tregs supernatants. Elevated protein expressions of IL-10 and TGF-β1 in regulatory T cells (Tregs) from burned patients were detected on postburn days (PBD) 1 to 21 in comparison to normal controls, and there were obvious differences among patients with different extent of burn injury ((a and b) a mean of all days). Protein levels of (c) IL-10 and (d) TGF-β1 in Tregs were significantly higher in septic patients than those without sepsis on PBD 3 to 21. Among septic patients, (e) IL-10 and (f) TGF-β1 levels in the survivors were obviously lower than those with non-survivors on PBD 3 to 21. **P < 0.01, Group I vs. normal group, or sepsis group vs. non-sepsis group or non-survivors group vs. survivors group; ##P < 0.01, Group II vs. Group I; &P < 0.05, &&P < 0.01, Group III vs. Group II.
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Figure 3: ELISA analysis of IL-10 and TGF-β1 levels in Tregs supernatants. Elevated protein expressions of IL-10 and TGF-β1 in regulatory T cells (Tregs) from burned patients were detected on postburn days (PBD) 1 to 21 in comparison to normal controls, and there were obvious differences among patients with different extent of burn injury ((a and b) a mean of all days). Protein levels of (c) IL-10 and (d) TGF-β1 in Tregs were significantly higher in septic patients than those without sepsis on PBD 3 to 21. Among septic patients, (e) IL-10 and (f) TGF-β1 levels in the survivors were obviously lower than those with non-survivors on PBD 3 to 21. **P < 0.01, Group I vs. normal group, or sepsis group vs. non-sepsis group or non-survivors group vs. survivors group; ##P < 0.01, Group II vs. Group I; &P < 0.05, &&P < 0.01, Group III vs. Group II.

Mentions: The capacity of Tregs to produce IL-10 and TGF-β1, which are two of the markers of function of Tregs, was analyzed in the present experiment. As shown in Figures 3 and 4, elevated protein and gene expressions of IL-10 and TGF-β1 in Tregs from burned patients were detected on PBD 1 to 21 in comparison with normal controls, and there were marked differences among patients with different extents of burn injury (P < 0.05 or P < 0.01). The protein and gene expressions of IL-10 and TGF-β1 in Tregs were significantly higher in septic patients than those without sepsis on PBD 3 to 21 (P < 0.05 or P < 0.01). Among septic patients, the expression levels of IL-10 and TGF-β1 in the survival group were obviously lower than those with non-survival group on PBD 3 to 21 (P < 0.05 or P < 0.01).


Association between regulatory T cell activity and sepsis and outcome of severely burned patients: a prospective, observational study.

Huang LF, Yao YM, Dong N, Yu Y, He LX, Sheng ZY - Crit Care (2010)

ELISA analysis of IL-10 and TGF-β1 levels in Tregs supernatants. Elevated protein expressions of IL-10 and TGF-β1 in regulatory T cells (Tregs) from burned patients were detected on postburn days (PBD) 1 to 21 in comparison to normal controls, and there were obvious differences among patients with different extent of burn injury ((a and b) a mean of all days). Protein levels of (c) IL-10 and (d) TGF-β1 in Tregs were significantly higher in septic patients than those without sepsis on PBD 3 to 21. Among septic patients, (e) IL-10 and (f) TGF-β1 levels in the survivors were obviously lower than those with non-survivors on PBD 3 to 21. **P < 0.01, Group I vs. normal group, or sepsis group vs. non-sepsis group or non-survivors group vs. survivors group; ##P < 0.01, Group II vs. Group I; &P < 0.05, &&P < 0.01, Group III vs. Group II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2875505&req=5

Figure 3: ELISA analysis of IL-10 and TGF-β1 levels in Tregs supernatants. Elevated protein expressions of IL-10 and TGF-β1 in regulatory T cells (Tregs) from burned patients were detected on postburn days (PBD) 1 to 21 in comparison to normal controls, and there were obvious differences among patients with different extent of burn injury ((a and b) a mean of all days). Protein levels of (c) IL-10 and (d) TGF-β1 in Tregs were significantly higher in septic patients than those without sepsis on PBD 3 to 21. Among septic patients, (e) IL-10 and (f) TGF-β1 levels in the survivors were obviously lower than those with non-survivors on PBD 3 to 21. **P < 0.01, Group I vs. normal group, or sepsis group vs. non-sepsis group or non-survivors group vs. survivors group; ##P < 0.01, Group II vs. Group I; &P < 0.05, &&P < 0.01, Group III vs. Group II.
Mentions: The capacity of Tregs to produce IL-10 and TGF-β1, which are two of the markers of function of Tregs, was analyzed in the present experiment. As shown in Figures 3 and 4, elevated protein and gene expressions of IL-10 and TGF-β1 in Tregs from burned patients were detected on PBD 1 to 21 in comparison with normal controls, and there were marked differences among patients with different extents of burn injury (P < 0.05 or P < 0.01). The protein and gene expressions of IL-10 and TGF-β1 in Tregs were significantly higher in septic patients than those without sepsis on PBD 3 to 21 (P < 0.05 or P < 0.01). Among septic patients, the expression levels of IL-10 and TGF-β1 in the survival group were obviously lower than those with non-survival group on PBD 3 to 21 (P < 0.05 or P < 0.01).

Bottom Line: They were also higher in septic patients than those without sepsis.Among septic patients, the expressions of Tregs phenotypes and the levels of cytokines were markedly lower in the survival group than those in patients with fatal outcome.Severe burn injury per se could lead to the changes in Tregs activities.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, Burns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, 51 Fu-cheng Road, Haidian District, Beijing 100048, PR China. burnshlf@sina.com

ABSTRACT

Introduction: To investigate the significance of changes in regulatory T cells (Tregs) activity and its relationship with sepsis, as well as outcome of patients with major burns.

Methods: The periphery blood samples of 106 patients were collected on post-burn days 1, 3, 7, 14, and 21. Tregs were isolated and their phenotypes (cytotoxic T-lymphocyte-associated antigen 4 and forkhead/winged helix transcription factor p3) were analyzed by flow cytometry, and the contents of cytokines (interleukin-10 and transforming growth factor-beta1) released into supernatants by Tregs were also determined by enzyme-linked immunosorbent assay kits. Gene expressions of cytokines were assessed by real-time quantitative polymerase chain reaction.

Results: Expressions of Tregs phenotypes and gene/protein expression of cytokines were all elevated after burn, and there were obvious differences among patients with various burn sizes. They were also higher in septic patients than those without sepsis. Among septic patients, the expressions of Tregs phenotypes and the levels of cytokines were markedly lower in the survival group than those in patients with fatal outcome.

Conclusions: Severe burn injury per se could lead to the changes in Tregs activities. Elevated levels of cytokines produced by Tregs and activation markers on Tregs surface might play an important role in the pathogenesis of sepsis and mortality in burned patients.

Show MeSH
Related in: MedlinePlus